Synthesis andin vitrosafety assessment of magnetic bacterial cellulose with porcine aortic smooth muscle cells

Abstract

Bacterial cellulose (BC) has been used as a scaffold for tissue regeneration (TR). Improving functional TR requires highly selective strategies for specific cell attraction. Embedding iron oxide nanoparticles into a BC matrix can drive magnetically labeled cells to specific tissues where they may begin to heal injured tissue. This article focuses on characterization and in vitro toxicity assessment of magnetic BC (MBC). We proposed to detect the production of radical oxygen species (ROS), esterase activity, and apoptosis to study cytotoxic interactions of MBC within its bioenvironment. Morphological characterization was performed using scanning electron microscopy where evidence shows that the diameter of MBC fibers compared to BC fibers was 33% smaller, and the pore areas were 25% bigger. Cytotoxicity assays in porcine aortic smooth muscle cells exposed for 24 hours to BC, MBC, and poly(ethylene glycol)-coated MBC (MBC-PEG) reveals 96% viability and 9% ROS production for MBC-PEG. In contrast, 25% of cells exposed to MBC were apoptotic, suggesting that even when the cells were metabolically active, MBC can induce damage. These outcomes support the need for more integral assessment in the hopes of assessing the potential biosafety and uses of nanocomposites for TR. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2801-2809, 2016.

Physical and tensile properties of epoxy laminated magnetic bacterial cellulose nanocomposite films

“…A variety of methods have been developed for the synthesis and mixing of magnetic nanoparticles in polymer matrices; each has advantages and limitations. “ In situ synthesis” is one of the efficient methods to achieve magnetic nanoparticles with uniform particle size distribution and proper dispersion in hydrophilic polymer substrates such as poly(vinyl alcohol) and cellulose . Various types of plant cellulose fibers, nanofibrillated cellulose (NFC), bacterial cellulose, regenerated cellulose, cellulose derivatives, and even cellulose nanocrystals have been used as substrates for magnetic nanoparticles.…”

“…"In situ synthesis" is one of the efficient methods to achieve magnetic nanoparticles with uniform particle size distribution and proper dispersion in hydrophilic polymer substrates such as poly(vinyl alcohol) 1,14-16 and cellulose. 2,[6][7][8][9][10][11]13,[17][18][19][20][21] Various types of plant cellulose fibers, 9,10,13,21 nanofibrillated cellulose (NFC), 22,23 bacterial cellulose, 2,11 regenerated cellulose, 19,24 cellulose derivatives, 25,26 and even cellulose nanocrystals 18 have been used as substrates for magnetic nanoparticles.…”

“…Magnetic nanoparticles have high potential applications in various fields including biotechnology and medicine, electronic, environment, and so on. Therefore, materials scientists have paid particular attention to this category of nanomaterials in recent years.…”

Biocompatible HA@Fe3O4@N‐CDs hybrids for detecting and absorbing lead ion

“…Feng's group synthesized magnetic HA nanoparticles as an adsorbent for the removal of Cd 2+ and Zn 2+ from aqueous solution . Furthermore, the incorporation of the magnetic Fe 3 O 4 particles made the separation and recycling more efficient and more eco‐friendly …”

“…26 Furthermore, the incorporation of the magnetic Fe 3 O 4 particles made the separation and recycling more efficient and more eco-friendly. 27,28 Over the past years, carbon dots (CDs) have excellent applications in biomedicine, ion detection, and bioimaging due to their novel electronic and physicochemical characteristics, such as strong fluorescence, low toxicity, and easy functionalization. [29][30][31] In particular, CDs can be applied to remove of anionic organic dye owing to CDs contain numerous oxygen-containing functional groups.…”