Synthesis and Cytotoxicity of Dendritic Platinum Nanoparticles with HEK‐293 Cells

Shim, Kyubin; Kim, Jeonghun; Heo, Yoon-Uk; Jiang, Bo; Li, Cuiling; Shahabuddin, Mohammed; Wu, Kevin Chien-Chang; Hossain, Md. Shahriar A.; Yamauchi, Yusuke; Kim, Jung Ho

doi:10.1002/asia.201601239

Cited by 29 publications

(26 citation statements)

References 52 publications

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“…Due to this, the reduction of Au ions preferentially forms Au cores as seeds in a short time, and then, Pt nanoparticles grow onto the Au core seeds. Brij plays an important role as a structure-directing agent for the formation of the nanomaterials with AA [33,34]. In the second step, additional Au NPs were incorporated into the porous Pt shells of the Au@Pt NPs using the potential step method.…”

Section: Resultsmentioning

confidence: 99%

Au decorated core-shell structured Au@Pt for the glucose oxidation reaction

Shim

Lee

Park

et al. 2019

Sensors and Actuators B: Chemical

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Highlights  • Bimetallic Au@Pt nanoparticles as core-shell structures demonstrate the enhanced catalytic performance for glucose oxidation.  • Nafion layer and dendritic Pt shells prevent passivation of the Au core and additional Au plating further enhances catalytic performance.  • Rationally designed Au-decorated Au@Pt NPs show enhanced sensitivity, stability, and selectivity towards glucose detection.

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Section: Resultsmentioning

confidence: 99%

Au decorated core-shell structured Au@Pt for the glucose oxidation reaction

Shim

Lee

Park

et al. 2019

Sensors and Actuators B: Chemical

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“…The methodology for synthesizing the nanoparticles was adapted from Shim et al 25 As described below, the morphology, structure, and size of the nanoparticles was characterized using Transmission electron microscopy (TEM) (JEM 1010, JEOL, United States) with an acceleration voltage at 80Kev. The composition of the nanoparticles was measured by Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDX) (Hitachi S-4800, Hitachi, Japan).…”

Section: Characterization Synthesis and Characterization Of The Nanopmentioning

confidence: 99%

“…Cells were cultured in a 96-well plate at a concentration of 50,000 cells/mL and then were incubated for 24 hrs at 37°C. After the incubation, the media was replaced with different concentrations of nanoparticles (10,25,50,75,100,150,200 and 250 µg/mL) in cell media and incubated for 1, 3 or 5 days. After incubating for the time required (the media was not changed between these days), the viability of the cells was determined by removing media and adding the MTS solution, which was then incubated for 3 hrs at 37°C.…”

Section: Cytotoxicitymentioning

confidence: 99%

<p>Novel Silver-Platinum Nanoparticles for Anticancer and Antimicrobial Applications</p>

Ruiz¹,

Garcia²,

Gallón³

et al. 2020

IJN

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Background: Cancer is a disease with an enormous worldwide impact. One of the fatal complications in cancer patients are bacterial opportunistic infections. The use of chemotherapeutic drugs made cancer remission more frequent and prolonged patient survival, but, increased the risk of infections. Purpose: Address the current problem with growing pandemic cancer and considering high risks of complications with bacterial infections, the present study synthesized novel dendritic assembly of silver (Ag)-platinum (Pt) nanoparticles. Methods: Nanoparticles were characterized by TEM analysis, and the composition was confirmed by EDX. Bacterial studies were performed for Gram-positive Staphylococcus aureus, Gram-negative Pseudomonas aeruginosa and Gram-negative multi-drug resistant Escherichia coli. Cell experiments were performed with two different cancer cell lines, glioblastoma and melanoma to determine anticancer activity. Finally, cytotoxicity with fibroblast was tested. Results: The TEM analysis of silver-platinum (AgPt) nanoparticles showed dendrimer shape nanoparticles with a mean size of 42 ± 11nm. Elemental composition was analyzed by EDX, confirming the presence of both Ag and Pt metals. The synthesized nanoparticles significantly inhibited the growth of medically important pathogenic, Gram-positive Staphylococcus aureus, Gram-negative Pseudomonas aeruginosa and Gram-negative multi-drug resistant Escherichia coli. Bactericidal effect of AgPt nanoparticles had greater effectiveness than silver nanoparticles. MTS assay revealed a selective and dose-dependent anticancer activity of AgPt nanoparticles over cancer cell lines glioblastoma and melanoma in the 10-250 µg/mL concentration range. Cytotoxicity experiments with fibroblast cells showed no side effects of nanoparticles against healthy cells at a range of concentrations from 10-50 µg/mL. Conclusion: The newly synthesized AgPt nanoparticles have a promising future as a potent anticancer agent with antibacterial properties.

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“…Surface area and crystallinity also affect their properties 1 . Among these NPs, noble metals, including Pt, Au, Ag, and Pd, are particularly important for catalysts 2,3 , sensors 4,5 , photonics 6,7 , and medicine 8,9 . As explained in one of the most extensive studies, Pt NPs are highly attractive due to their superior catalytic activity, despite their high cost 2 .…”

Section: Introductionmentioning

confidence: 99%

Rationally designed bimetallic Au@Pt nanoparticles for glucose oxidation

Shim

Lee

Heo

et al. 2019

Sci Rep

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Bimetallic nanoparticles (NPs) have aroused interest in various fields because of their synergetic and unique properties. Among those nanoparticles, we strategically approached and synthesized Au@Pt NPs via the sonochemical method with different molar ratios (e.g. 3:7, 5:5, and 7:3) of Au to Pt precursors. The particle structure was confirmed to be core-shell, and the size was estimated to be 60, 52, and 47 nm, respectively, for 3:7, 5:5, and 7:3 ratios of Au to Pt. The detailed structure and crystallinity of as-prepared Au@Pt NPs were further studied by scanning electron microscopy, transmission electron microscopy with element mapping, and X-ray diffraction. It should be noted that thickness of the dendritic Pt shell in the core-shell structure can be easily tuned by controlling the molar ratio of Au to Pt. To explore the possibility of this material as glucose sensor, we confirmed the detection of glucose using amperometry. Two dynamic ranges in a calibration plot were displayed at 0.5–50.0 µM and 0.05–10.0 mM, and their detection limit as glucose sensor was determined to be 319.8 (±5.4) nM.

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Synthesis and Cytotoxicity of Dendritic Platinum Nanoparticles with HEK‐293 Cells

Cited by 29 publications

References 52 publications

Au decorated core-shell structured Au@Pt for the glucose oxidation reaction

Au decorated core-shell structured Au@Pt for the glucose oxidation reaction

<p>Novel Silver-Platinum Nanoparticles for Anticancer and Antimicrobial Applications</p>

Rationally designed bimetallic Au@Pt nanoparticles for glucose oxidation

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