Synthesis and characterization of electrospun PVA/Zn2+ metal composite nanofibers for lipase immobilization with effective thermal, pH stabilities and reusability

Işık, Ceyhun; Arabacı, Gökmen; Doğaç, Yasemin İspirli; Deveci, İlyas; Teke, Mustafa

doi:10.1016/j.msec.2019.02.031

Cited by 57 publications

(15 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, Zn 2 + -doped PVA nanofibers were produced by the electrospinning method. [33] Then, AChE was immobilized on Zn 2 + -doped PVA nanofibers by adsorption and cross-linking (Scheme 1). Optimization parameters such as adsorption time, amount of AChE, nanofiber and glutaraldehyde (GA) were determined for AChE immobilization.…”

Section: Optimization Studiesmentioning

confidence: 99%

“…In addition, Zn 2 + in the nanofiber structure may have positively affected the interactions between the enzyme and the nanofiber and provided a microenvironment around the enzyme's active site that would reduce the effect of heat on the enzyme. [33] There are many investigations in the literature on thermal stability increases after immobilization. Stoilova et al reported that AChE immobilized NaOH/ ethylenediamine activated polyacrylonitrile nanofiber kept approximately 50 % activity at 65°C after 60 min, while free AChE protected only 23 % activitiy.…”

Section: Temperature Propertiesmentioning

confidence: 99%

“…In a previous study, we performed lipase immobilization on PVA/Zn 2 + nanofibers. [33] To our knowledge, there is no other immobilization study on these nanofibers and the AChE enzyme was immobilized on Zn 2 + -doped PVA nanofibers for the first time. AChE immobilized on Zn 2 + -doped PVA nanofibers was prepared for investigating to improve stability properties of AChE such as optimum temperature, optimum pH, reusability, kinetic parameters, thermal and pH stability.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, AChE was immobilized on electrospun Zn 2+ ‐doped PVA nanofibers via adsorption and cross‐linking methods. In a previous study, we performed lipase immobilization on PVA/Zn 2+ nanofibers [33] . To our knowledge, there is no other immobilization study on these nanofibers and the AChE enzyme was immobilized on Zn 2+ ‐doped PVA nanofibers for the first time.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Zn²⁺‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**

et al. 2020

Self Cite

View full text Add to dashboard Cite

Enzyme immobilization provides improve mechanical characteristic of enzymes and stability to environmental alters. It also helps to enzymes maintain their activities for a long time and offers the possibility to use them again. With these unique features, they can reduce production costs in industrial applications. In this study, acetylcholinesterase (AChE) immobilized on Zn2+‐doped PVA nanofibers via adsorption and cross‐linking methods. Morphological structures of Zn2+‐doped PVA nanofibers were characterized by Scanning Electron Microscopy (SEM) before and after immobilization. It was observed that the enzyme gained resistance against temperature and pH changes occurring in the reaction medium after immobilization process. In addition, immobilized Zn2+‐doped PVA nanofibers retained of its activity approximately 75 % after 8 reuse and its activity decreased below 50 % after 12 reuse. As a result, AChE immobilized Zn2+‐doped PVA nanofibers offers numerous advantages for various biotechnological applications, such as hydrolysis and determination of pesticides, use in the structure of reactors and electrodes, thanks to the unique stability and reusability features that AChE gains after immobilization.

show abstract

Section: Optimization Studiesmentioning

confidence: 99%

Section: Temperature Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Zn²⁺‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Eco-friendly fibrous materials for environmental air filtration can be designed to feature high filtration efficiency, high tensile strength, biocompatibility, and antibacterial properties [2] . Many of these materials contain poly(vinyl alcohol) (PVA), which is commonly processed by electrospinning, features the advantages of non-toxicity, mechanical stability, biocompatibility, and excellent film-forming ability, and is extensively used as a component of air filtration devices, medical supplies, and food packaging [3] . Bioprotective materials typically contain metal nanoparticles, nanocellulose, and chitosan as antibacterial constituents [4] .…”

Section: Introductionmentioning

confidence: 99%

Electrospun antibacterial poly(vinyl alcohol)/Ag nanoparticles membrane grafted with 3,3′,4,4′-benzophenone tetracarboxylic acid for efficient air filtration

Zhang

Xie

et al. 2020

Applied Surface Science

View full text Add to dashboard Cite

show abstract

Overview on immobilization of enzymes on synthetic polymeric nanofibers fabricated by electrospinning

Rather

Khan

Wani

et al. 2021

Biotech & Bioengineering

View full text Add to dashboard Cite

The arrangement and type of support has a significant impact on the efficiency of immobilized enzymes. 1-dimensional fibrous materials can be one of the most desirable supports for enzyme immobilization. This is due to their high surface area to volume ratio, internal porosity, ease of handling, and high mechanical stability, all of which allow a higher enzyme loading, release and finally lead to better catalytic efficiency. Fortunately, the enzymes can reside inside individual nanofibers to remain encapsulated and retain their three-dimensional structure. These properties can protect the enzyme's tolerance against harsh conditions such as pH variations and high temperature, and this can probably enhance the enzyme's stability. This review article will discuss the immobilization of enzymes on synthetic polymers, which are fabricated into nanofibers by electrospinning. This technique is rapidly gaining popularity as one of the most practical ways to fibricate polymer, metal oxide, and composite micro or nanofibers. As a result, there is interest in using nanofibers to immobilize enzymes. Furthermore, present research on electrospun nanofibers for enzyme immobilization is primarily limited to the lab scale and industrial scale is still challanging. The primary future research objectives of this paper is to investigate the use of electrospun nanofibers for enzyme immobilization, which includes increasing yield to transfer biological products into commercial applications.

show abstract

Synthesis and characterization of electrospun PVA/Zn2+ metal composite nanofibers for lipase immobilization with effective thermal, pH stabilities and reusability

Cited by 57 publications

References 79 publications

Zn²⁺‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**

Zn²⁺‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**

Electrospun antibacterial poly(vinyl alcohol)/Ag nanoparticles membrane grafted with 3,3′,4,4′-benzophenone tetracarboxylic acid for efficient air filtration

Overview on immobilization of enzymes on synthetic polymeric nanofibers fabricated by electrospinning

Contact Info

Product

Resources

About

Synthesis and characterization of electrospun PVA/Zn2+ metal composite nanofibers for lipase immobilization with effective thermal, pH stabilities and reusability

Cited by 57 publications

References 79 publications

Zn2+‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**

Zn2+‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**

Electrospun antibacterial poly(vinyl alcohol)/Ag nanoparticles membrane grafted with 3,3′,4,4′-benzophenone tetracarboxylic acid for efficient air filtration

Overview on immobilization of enzymes on synthetic polymeric nanofibers fabricated by electrospinning

Contact Info

Product

Resources

About

Zn²⁺‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**

Zn²⁺‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase**