Synthesis and characterization of cell-laden double-network hydrogels based on silk fibroin and methacrylated hyaluronic acid

Xiao, Wenqian; Qu, Xiaohang; Li, Jiale; Chen, Lin; Tan, Yunfei; Li, Kejiang; Li, Bo; Liao, Xiaoling

doi:10.1016/j.eurpolymj.2019.05.040

Cited by 24 publications

(22 citation statements)

References 49 publications

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“…However, they did not perform the rheological study for the aforementioned hydrogels [23]. Recently, using sonication and UV photo-polymerization to prepare SF/methacrylated HA or to produce SF-based IPN hydrogels has been reported by Xiao et al [33], respectively. However, the rheological properties of the hydrogels were not determined.…”

Section: Methodsmentioning

confidence: 99%

Roles of Silk Fibroin on Characteristics of Hyaluronic Acid/Silk Fibroin Hydrogels for Tissue Engineering of Nucleus Pulposus

Chung¹,

Chen

Tai³

et al. 2020

Materials

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Silk fibroin (SF) and hyaluronic acid (HA) were crosslinked by horseradish peroxidase (HRP)/H2O2, and 1,4-Butanediol di-glycidyl ether (BDDE), respectively, to produce HA/SF-IPN (interpenetration network) (HS-IPN) hydrogels. HS-IPN hydrogels consisted of a SF strain with a high content of tyrosine (e.g., strain A) increased viscoelastic modules compared with those with low contents (e.g., strain B and C). Increasing the quantities of SF in HS-IPN hydrogels (e.g., HS7-IPN hydrogels with weight ratio of HA/SF, 5:7) increased viscoelastic modules of the hydrogels. In addition, the mean pores size of scaffolds of the model hydrogels were around 38.96 ± 5.05 μm which was between those of scaffolds H and S hydrogels. Since the viscoelastic modulus of the HS7-IPN hydrogel were similar to those of human nucleus pulposus (NP), it was chosen as the model hydrogel for examining the differentiation of human bone marrow-derived mesenchymal stem cell (hBMSC) to NP. The differentiation of hBMSC induced by transforming growth factor β3 (TGF-β3) in the model hydrogels to NP cells for 7 d significantly enhanced the expressions of glycosaminoglycan (GAG) and collagen type II, and gene expressions of aggrecan and collagen type II while decreased collagen type I compared with those in cultural wells. In summary, the model hydrogels consisted of SF of strain A, and high concentrations of SF showed the highest viscoelastic modulus than those of others produced in this study, and the model hydrogels promoted the differentiation of hBMSC to NP cells.

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Section: Methodsmentioning

confidence: 99%

Roles of Silk Fibroin on Characteristics of Hyaluronic Acid/Silk Fibroin Hydrogels for Tissue Engineering of Nucleus Pulposus

Chung¹,

Chen

Tai³

et al. 2020

Materials

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“…In another study, Zelong Chen et al 100 prepared a new system of superparamagnetic iron oxide (USPIO) labelled cellulose nanocrystal/silk fibroin functional hydrogel, which allowed non-invasive monitoring of the hydrogel degradation during cartilage regeneration by using MRI (figure 10b). Wenqian Xiao 101 developed a silk fibroin/methacrylate hyaluronic acid (SF-HAMA) doublenetwork hydrogel by combining sonication and UV irradiation. The hydrogel demonstrated high mechanical strength and excellent biocompatibility, thus, confirming its suitability for the Please do not adjust margins Please do not adjust margins load-bearing soft tissue.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Functional silk fibroin hydrogels: preparation, properties and applications

2021

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“…The double bond renders the compound highly sensitive to UV radiation and consequently to chain polymerization. One of the most researched photosynthesized hydrogels is methacrylated hyaluronic acid [ 43 , 44 , 45 ]. It serves well in biomedical applications and is synthesized through the UV radiation of methacrylated hyaluronic acid prepolymer solution in the presence of a photoinitiator.…”

Section: Types Of Hydrogel Networkmentioning

confidence: 99%

Relationship between Structure and Rheology of Hydrogels for Various Applications

Stojkov

Niyazov

Picchioni

et al. 2021

Gels

242

117

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Hydrogels have gained a lot of attention with their widespread use in different industrial applications. The versatility in the synthesis and the nature of the precursor reactants allow for a varying range of hydrogels with different mechanical and rheological properties. Understanding of the rheological behavior and the relationship between the chemical structure and the resulting properties is crucial, and is the focus of this review. Specifically, we include detailed discussion on the correlation between the rheological characteristics of hydrogels and their possible applications. Different rheological tests such as time, temperature and frequency sweep, among others, are described and the results of those tests are reported. The most prevalent applications of hydrogels are also discussed.

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Synthesis and characterization of cell-laden double-network hydrogels based on silk fibroin and methacrylated hyaluronic acid

Cited by 24 publications

References 49 publications

Roles of Silk Fibroin on Characteristics of Hyaluronic Acid/Silk Fibroin Hydrogels for Tissue Engineering of Nucleus Pulposus

Roles of Silk Fibroin on Characteristics of Hyaluronic Acid/Silk Fibroin Hydrogels for Tissue Engineering of Nucleus Pulposus

Functional silk fibroin hydrogels: preparation, properties and applications

Relationship between Structure and Rheology of Hydrogels for Various Applications

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