Synthesis and characterization of bioactive hydroxyapatite–calcite nanocomposite for biomedical applications

Kumar, G. Suresh; Girija, E. K.; Thamizhavel, A.; Yokogawa, Yoshiyuki; Kalkura, S. Narayana

doi:10.1016/j.jcis.2010.05.038

Cited by 94 publications

(49 citation statements)

References 40 publications

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“…narrow OH stretch band is associated with free OH ions [Elliott, 1994;Coates, 2000;Wilson et al, 2003] and the broad band with the existence of lattice or adsorbed water in the samples [Slósarczyk et al, 1997;Xu et al, 1998;Koumoulidis et al, 2003;Wilson et al, 2003;Kumar et al, 2010]. As the fluoride group, the intensity of the narrow OH (3,570 cm -1 ) also decreased in the groups treated with iron ( fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Delbem¹,

Alves²,

Sassaki

et al. 2012

Caries Res

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The aim of this study was to evaluate the effect of iron II on the dissolution and precipitation of synthetic hydroxyapatite (HA). HA powder was suspended in solutions of iron (0.84 µg/ml, Fe0.84; 18.0 µg/ml, Fe18; 70.0 µg/ml, Fe70), fluoride (1,100 µg/ml, F1,100), and deionized water and submitted to pH cycling. After pH cycling, the samples were analyzed by infrared spectroscopy and X-ray diffraction. The concentrations of fluoride, calcium, phosphorus, and iron were also analyzed. The data were submitted to ANOVA, and analyzed by Tukey’s test (p < 0.05). The infrared spectrum showed a reduction in all bands corresponding to phosphates and hydroxyls and an increase in the carbonate band in the groups with iron. The intensity of the phosphate bands increased and that of the hydroxyl bands decreased in the group F1,100. It was observed that there was a higher concentration of Ca in the group F1,100, with no significant difference between the groups Fe18 and Fe70 (p > 0.05). There was an increase in Fe concentration in the HA directly related to the Fe concentration of the treatment solutions. Results show that the presence of Fe causes the precipitation of apatite with high solubility.

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Section: Discussionmentioning

confidence: 99%

Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Delbem¹,

Alves²,

Sassaki

et al. 2012

Caries Res

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“…57 The weight loss of PG/HA and polymer coated PG/HA composite scaffolds with incubation in SBF until 28 th day is shown in Fig. 6b.…”

Section: Degradationmentioning

confidence: 99%

Polymer coated phosphate glass/hydroxyapatite composite scaffolds for bone tissue engineering applications

2015

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The development of bioactive ceramic composite scaffold materials with enhanced mechanical strength has been a topic of great interest in bone tissue engineering. In the present study, phosphate glass/hydroxyapatite (PG/HA) composite scaffold with an open-porous structure (∼89% porosity and pore size in the range 200−500 µm) has been fabricated by foam replica method. In order to enhance the mechanical strength of this scaffold a polymer coating of alginate, chitosan and gelatin was made on the composite scaffold by immersion method. The polymer coating did not affect the interconnectivity but reduced the porosity of the PG/HA composite scaffold. Biodegradation studies revealed that all the composite scaffolds have undergone significant degradation. But the compressive strength of the gelatin coated scaffold (G-PG/HA) exhibited sevenfold enhancement than the pristine scaffold. The PG/HA and G-PG/HA composite scaffolds revealed excellent biocompatibility with human osteoblast-like MG-63 cells. Hence, G-PG/HA composite scaffold may be a smart promising scaffold for bone tissue engineering.

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“…Hydroxyapatite (HAP) is the principal inorganic constituent of human bones and teeth, and its molecular formula is Ca 10 (PO 4 ) 6 (OH) 2 . 4 Synthetic HAP crystals are now widely used in medical implants and as coatings on prostheses. 5 Compared to viral vectors, which possess risks of pathogenicity and immunogenicity, synthetic HAP nanoparticles have favourable biocompatibility and high osteoconductivity and/or osteoinductivity without immunogenicity or proinflammatory features.…”

Section: Introductionmentioning

confidence: 99%

Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo

Zhu¹,

Guo²,

Li³

et al. 2011

Asian J Androl

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DNA vector-based Stat3-specific RNA interference (si-Stat3) blocks Stat3 signalling and inhibits prostate tumour growth. However, the antitumour activity depends on the efficient delivery of si-Stat3. The effects on the growth of mouse prostate cancer cells of si-Stat3 delivered by hydroxyapatite were determined in this study. RM-1 tumour blocks were transplanted into C57BL/6 mice. CaCl 2 -modified hydroxyapatite carrying si-Stat3 plasmids were injected into tumours, and tumour growth and histology were determined. The expression levels of Stat3, pTyr-Stat3, Bcl-2, Bax, Caspase3, VEGF and cyclin D1 were measured by western blot analysis. Amounts of apoptosis in cancer cells were analysed with immunohistochemistry and the terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) assay. The results showed that hydroxyapatite-delivered si-Stat3 significantly suppressed tumour growth up to 74% (P,0.01). Stat3 expression was dramatically downregulated in the tumours. The immunohistochemistry and TUNEL results showed that si-Stat3-induced apoptosis (up to 42%, P,0.01). The Stat3 downstream genes Bcl-2, VEGF and cyclin D1 were also strongly downregulated in the tumour tissues that also displayed significant increases in Bax expression and Caspase3 activity. These results suggest that hydroxyapatite can be used for the in vivo delivery of plasmid-based siRNAs into tumours.

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Synthesis and characterization of bioactive hydroxyapatite–calcite nanocomposite for biomedical applications

Cited by 94 publications

References 40 publications

Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Polymer coated phosphate glass/hydroxyapatite composite scaffolds for bone tissue engineering applications

Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo

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