Synergistic Influence of Polypeptide Growth Factors on Cultured Human Muscle

Epidermal Growth Factor Induces Differentiation and Secretion of Human Chorionic Gonadotropin and Placental Lactogen in Normal Human Placenta*

“…Studies of EGF-induced differentiation of human epidermal keratinocytes, nasal mucosa cells, and smooth muscle cells have all used undefined growth-promoting substances, such as serum, feeder-layers, and pituitary or hypothalamic extracts (35)(36)(37)(38)(39)(40)(41)(42).…”

Epidermal Growth Factor Induces Differentiation and Secretion of Human Chorionic Gonadotropin and Placental Lactogen in Normal Human Placenta*

“…Studies of EGF-induced differentiation of human epidermal keratinocytes, nasal mucosa cells, and smooth muscle cells have all used undefined growth-promoting substances, such as serum, feeder-layers, and pituitary or hypothalamic extracts (35)(36)(37)(38)(39)(40)(41)(42).…”

Missense mutations have unexpected consequences: The McArdle disease paradigm

“…Polysomal profiling and RNA analysis was performed as detailed elsewhere (Martinez‐Azorin, Remacha, & Ballesta, ). Myoblasts were isolated from biopsies of P40 and a control subject by explant culture (Askanas & Gallez‐Hawkins, ). Retrotranscription was done using the SuperScript III First‐Strand Synthesis System (Invitrogen, Carlsbad, CA), and amplification of the cDNA corresponding to the PYGM gene (NM_005609.2) was performed in two fragments as previously reported (Garcia‐Consuegra et al., ).…”

“…Polysomal profiling and RNA analysis was performed as detailed elsewhere (Martinez-Azorin, Remacha, & Ballesta, 2008). Myoblasts were isolated from biopsies of P40 and a control subject by explant culture (Askanas & Gallez-Hawkins, 1985). Retrotranscription was done…”

Comparative gene expression profiling between human cultured myotubes and skeletal muscle tissue

“…Biopsies were embedded into RNAlater for RNA extraction or into cell culture medium. Cultures were prepared through an explant technique [11,17]. Briefly, SM biopsy pieces were dissected under a stereomicroscope; fat, connective tissue and blood were removed; and the pieces were frozen in DMEM medium with 25% fetal bovine serum (FBS) and 6% DMSO.…”

Epidermal Growth Factor Induces Differentiation and Secretion of Human Chorionic Gonadotropin and Placental Lactogen in Normal Human Placenta*

“…Studies of EGF-induced differentiation of human epidermal keratinocytes, nasal mucosa cells, and smooth muscle cells have all used undefined growth-promoting substances, such as serum, feeder-layers, and pituitary or hypothalamic extracts (35)(36)(37)(38)(39)(40)(41)(42).…”

Missense mutations have unexpected consequences: The McArdle disease paradigm

“…Polysomal profiling and RNA analysis was performed as detailed elsewhere (Martinez‐Azorin, Remacha, & Ballesta, ). Myoblasts were isolated from biopsies of P40 and a control subject by explant culture (Askanas & Gallez‐Hawkins, ). Retrotranscription was done using the SuperScript III First‐Strand Synthesis System (Invitrogen, Carlsbad, CA), and amplification of the cDNA corresponding to the PYGM gene (NM_005609.2) was performed in two fragments as previously reported (Garcia‐Consuegra et al., ).…”

“…Polysomal profiling and RNA analysis was performed as detailed elsewhere (Martinez-Azorin, Remacha, & Ballesta, 2008). Myoblasts were isolated from biopsies of P40 and a control subject by explant culture (Askanas & Gallez-Hawkins, 1985). Retrotranscription was done…”

Comparative gene expression profiling between human cultured myotubes and skeletal muscle tissue

“…Biopsies were embedded into RNAlater for RNA extraction or into cell culture medium. Cultures were prepared through an explant technique [11,17]. Briefly, SM biopsy pieces were dissected under a stereomicroscope; fat, connective tissue and blood were removed; and the pieces were frozen in DMEM medium with 25% fetal bovine serum (FBS) and 6% DMSO.…”

Epidermal Growth Factor Induces Differentiation and Secretion of Human Chorionic Gonadotropin and Placental Lactogen in Normal Human Placenta*

“…Studies of EGF-induced differentiation of human epidermal keratinocytes, nasal mucosa cells, and smooth muscle cells have all used undefined growth-promoting substances, such as serum, feeder-layers, and pituitary or hypothalamic extracts (35)(36)(37)(38)(39)(40)(41)(42).…”

Missense mutations have unexpected consequences: The McArdle disease paradigm

“…Polysomal profiling and RNA analysis was performed as detailed elsewhere (Martinez‐Azorin, Remacha, & Ballesta, ). Myoblasts were isolated from biopsies of P40 and a control subject by explant culture (Askanas & Gallez‐Hawkins, ). Retrotranscription was done using the SuperScript III First‐Strand Synthesis System (Invitrogen, Carlsbad, CA), and amplification of the cDNA corresponding to the PYGM gene (NM_005609.2) was performed in two fragments as previously reported (Garcia‐Consuegra et al., ).…”

“…Polysomal profiling and RNA analysis was performed as detailed elsewhere (Martinez-Azorin, Remacha, & Ballesta, 2008). Myoblasts were isolated from biopsies of P40 and a control subject by explant culture (Askanas & Gallez-Hawkins, 1985). Retrotranscription was done…”

Comparative gene expression profiling between human cultured myotubes and skeletal muscle tissue

“…Biopsies were embedded into RNAlater for RNA extraction or into cell culture medium. Cultures were prepared through an explant technique [11,17]. Briefly, SM biopsy pieces were dissected under a stereomicroscope; fat, connective tissue and blood were removed; and the pieces were frozen in DMEM medium with 25% fetal bovine serum (FBS) and 6% DMSO.…”