Synergistic effects of electrospun PLLA fiber dimension and pattern on neonatal mouse cerebellum C17.2 stem cells

He, Liumin; Liao, Susan; Quan, Daping; Ma, Kun; Chan, Casey K.; Ramakrishna, Seeram; Lu, Jiang

doi:10.1016/j.actbio.2010.02.039

Cited by 148 publications

(112 citation statements)

References 51 publications

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“…He et al fabricated aligned PLLA fibers by electrospinning and showed variable differentiations of NSCs depending on their fiber diameter and alignment. They reported longer neuritis on aligned fibers than on random fibers within the same diameter range [191]. Christopherson et al cultured rat NSCs on electrospun polyethersulfone (PES) fibers with varying fiber diameters and demonstrated that the fiber diameter of PES fibers significantly influenced NSC differentiation.…”

Section: Alternative Stem Cells On Nanostructures For Nerve Regenerationmentioning

confidence: 98%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

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Stem cells are a promising alternative for cell therapy applications because of their self-renewing capability and potential to differentiate into a wide range of specialized cell lineages, including osteoblasts, chondrocytes, cardiomyocytes, and neuronal cells. Different kinds of stem cells are considered for cell-based tissue engineering approaches, of which bone-marrow-derived mesenchymal stem cells (BM-MSCs), adipose-derived stem cells (ADSCs) and embryonic stem cells (ESCs) are frequently utilized for advancement of new tissue engineering strategies. Nanostructures created by natural, synthetic, or composite polymeric biomaterials provide artificial templates of the extracellular matrix (ECM). They possess a high surface area to volume ratio, are porous with good mechanical properties, are sufficient to serve as a biomimetic platform to attract stem cells, cause differentiation, and provide functioning of the tissues. Nanoscale features such as fibers, pits, and grooves modulate cell behavior and might even stimulate the differentiation of stem cells to specific lineages; for example, the nanotopographical cues combined with chemical cues influence the neuronal induction of MSCs, resulting in high microtubule-associated protein expressions. Scaffolds can also be impregnated with several ligands for adhesion of receptors to the cells, or with other regulatory molecules and growth M.P. Prabhakaran (*) and J. Venugopal E3-05-12, Health Care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore e-mail: nnimpp@nus.edu.sg L. Ghasemi-Mobarakeh Islamic Azad University, Najafabad Branch, Isfahan, Iran D. Kai NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore G. Jin and S. Ramakrishna Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore factors for designed cell behavior or controlled cell differentiations. Different polymeric blends or fibers incorporated with nanoparticles (such as hydroxyapatite) in aligned or custom-made patterns can induce specific differentiation of stem cells for bone, cartilage, cardiac, nerve, and skin tissue regeneration. In this review, we will discuss the current state of the art and future perspectives on stem cells and their differentiation on nanoengineered substrates for advanced tissue regeneration.

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Section: Alternative Stem Cells On Nanostructures For Nerve Regenerationmentioning

confidence: 98%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

View full text Add to dashboard Cite

show abstract

“…Electrospun poly(llactide) (PLLA) fibers with different parameters were tested in culture with NSPC clone C17.2. The cells displayed significantly different growth and differentiation depending on fiber pattern and dimension they adhered (He et al, 2010). PLLA can also be modified by tethering laminin-deirved peptides through a cross-linking reagent, and the modified PLLA showed significant improvement in supporting cell survival and neurite outgrowth of C17.2 cells (He et al, 2009).…”

Section: Testing Biomaterialsmentioning

confidence: 99%

Neural Stem/Progenitor Cell Clones as Models for Neural Development and Transplantation

Li¹,

Zhao²,

Shu³

et al. 2012

Neural Stem Cells and Therapy

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“…Our studies was focused mainly on the characterization of the samples manufacturing and investigation of mechanical properties of obtained structures. In a literature we can find the confirmation of the influence of fiber's diameter on improvement of cells proliferation and differentiation [13,[25][26][27]. Therefore, for the implementation of this work, we focused on mechanical behaviour of the scaffolds with two diameters of the fibers.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of 3D hybrid microfiber/nanofiber scaffolds for bone tissue engineering

Ostrowska¹,

Jaroszewicz²,

Zaczyńska³

et al. 2014

Bulletin of the Polish Academy of Sciences Technical Sciences

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Abstract. Fabrication of scaffolds for tissue engineering (TE) applications becomes a very important research topic in present days. The aim of the study was to create and evaluate a hybrid polymeric 3D scaffold consisted of nano and microfibers, which could be used for bone tissue engineering. Hybrid structures were fabricated using rapid prototyping (RP) and electrospinning (ES) methods. Electrospun nanofibrous mats were incorporated between the microfibrous layers produced by RP technology. The nanofibers were made of poly(L-lactid) and polycaprolactone was used to fabricate microfibers. The micro-and nanostructures of the hybrid scaffolds were examined using scanning electron microscopy (SEM). X-ray microtomographical (µCT) analysis and the mechanical testing of the porous hybrid structures were performed using SkyScan 1172 machine, equipped with a material testing stage. The scanning electron microscopy and micro-tomography analyses showed that obtained scaffolds are hybrid nanofibers/microfibers structures with high porosity and interconnected pores ranging from 10 to 500um. Although, connection between microfibrous layers and electrospun mats remained consistent under compression tests, addition of the nanofibrous mats affected the mechanical properties of the scaffold, particularly its elastic modulus. The results of the biocompatibility tests didn't show any cytotoxic effects and no fibroblast after contact with the scaffold showed any damage of the cell body, the cells had proper morphologies and showed good proliferation. Summarizing, using RP technology and electrospinning method it is possible to fabricate biocompatible scaffolds with controllable geometrical parameters and good mechanical properties.

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Synergistic effects of electrospun PLLA fiber dimension and pattern on neonatal mouse cerebellum C17.2 stem cells

Cited by 148 publications

References 51 publications

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Neural Stem/Progenitor Cell Clones as Models for Neural Development and Transplantation

Evaluation of 3D hybrid microfiber/nanofiber scaffolds for bone tissue engineering

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