Synergistic effect of carbodiimide and dehydrothermal crosslinking on acellular dermal matrix

Hu, Yang; Liu, Lan; Dan, Weihua; Dan, Nianhua; Gu, Zhipeng; Yu, Xixun

doi:10.1016/j.ijbiomac.2013.01.009

Cited by 56 publications

(55 citation statements)

References 30 publications

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“…Within the next 12 h, DHT/EDC membranes exhibited slower degradation than the DHT membrane, and the BG membrane was completely degraded. The results of the present study are consistent with the previous study 18) . Increasing the cross-linking density of collagen membranes may promote biostability.…”

Section: Discussionsupporting

confidence: 94%

“…Positive correlations between cross-linking density and tensile strength have been reported 18,21) . In the present study, the tensile strength of two experimental membranes was evaluated.…”

Section: Discussionmentioning

confidence: 90%

“…Although the DHT/EDC membrane has a higher cross-linking density than the DHT membrane, the tensile strength of the DHT/EDC membrane was lower in wet conditions. One possible reason for this phenomenon is that EDC treatment partially denatures the 3D architecture of membrane itself, causing the misalignment of side chains and leading to membrane brittleness 18,28) . Another possible reason could be that the re-hydration and lyophilization procedure leads to loose connection between collagen fibers 25) .…”

Section: Discussionmentioning

confidence: 99%

“…In vitro studies have demonstrated that DHT membranes showed favorable mechanical properties due to the formation of ester and amide bonds 18) . In a clinical study, Lee et al 19) reported that DHT membranes possessed more stable maintenance characteristics than non-crosslinked collagen membranes.…”

Section: Introductionmentioning

confidence: 99%

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Physiochemical properties and resorption progress of porcine skin-derived collagen membranes: In vitro and in vivo analysis

Kim

Lim³

et al. 2018

Dental Materials Journal

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The aim of the present study was to evaluate the physiochemical properties and resorption progress of two cross-linked, porcine skin-derived collagen membranes and compare their features with those of a membrane without cross-linking (Bio-Gide ® [BG], Geistlich Biomaterials, Wolhusen, Switzerland). Three porcine skin-derived collagen membranes, dehydrothermally (DHT) crosslinked (experimental), DHT and 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide (DHT/EDC) cross-linked (experimental) and BG were investigated for their morphology, enzyme resistance, and tensile strength in vitro and biodegradation in vivo. DHT and DHT/ EDC membranes exhibited irregular, interconnected macro-and micropores that formed a 3D mesh, whereas BG exhibited individual collagen fibrils interlaced to form coarse collagen strands. In enzyme resistance and tensile strength tests, DHT and DHT/EDC membranes demonstrated good resistance and mechanical properties compared with BG. In vivo, all three membranes were well integrated into the surrounding connective tissue. Thus, the DHT membrane exhibited its potential as a barrier membrane for guided bone and tissue regeneration.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physiochemical properties and resorption progress of porcine skin-derived collagen membranes: In vitro and in vivo analysis

Kim

Lim³

et al. 2018

Dental Materials Journal

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“…The porcine acellular dermal matrix (pADM) scaffolds and pure collagen used in this work were self-prepared according to our previous report 28,29 or invention patent (CN1569260). As shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Preparation and evaluation of a novel pADM-derived micro- and nano electrospun collagen membrane

Liu

Dan

et al. 2015

RSC Adv.

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As the three-dimensional (3D) architecture of porcine acellular dermal matrix (pADM) mimics the biological characteristics of a native extracellular matrix (ECM), it has been extensively utilized as a tissue scaffold. In this study, a novel pADM-derived micro-and nano electrospun collagen membrane (PDEC) was successfully prepared by the electrospinning technique, using porcine acellular dermal matrix (pADM) as the raw material and 1,1,1,3,3,3-hexafluoro-2-propanol (HIFP) as the solvent, and was characterized by scanning electron microscopy (SEM) analysis. In contrast, another collagen-derived electrospun collagen (CDEC) was also fabricated using pure porcine collagen as the raw material. The structure and composition of the PDEC and CDEC were measured by Fourier transform infrared (FTIR) spectroscopy, SDS-PAGE gel electrophoresis, specific intrinsic viscosity and atomic force microscopy (AFM). The results indicate that the structural integrity of the PDEC is almost maintained and only a small amount of the PDEC was destroyed into gelatin, while almost all of the CDEC was degraded. Moreover, the results of circular dichroism (CD) analysis also demonstrate that the PDEC possesses a higher content of a-helix structure but less b-turn structure. Additionally, the results from ultrasensitive differential scanning calorimetry (US-DSC) and XRD analysis also suggest that the thermal stability and crystallization of the PDEC have little differences compared to collagen. Furthermore, the mechanical properties of the PDEC are significantly enhanced compared to those of the CDEC. Above all, the results obtained in the MTT study indicate that the PDEC has almost the same good biological activity, in terms of cytotoxicity, as natural porcine collagen, and this activity is obviously superior to that of the CDEC. In conclusion, our study provides a new pADM-derived electrospun collagen in which the triple helical structure has seldom been damaged and thus it can be applied as a novel electrospun collagen scaffold for tissue engineering.

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Biomedical Applications of Collagen/Gelatin

Sarwan,

Narang,

Kumari

et al. 2024

Biopolymers for Biomedical Applications

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Synergistic effect of carbodiimide and dehydrothermal crosslinking on acellular dermal matrix

Cited by 56 publications

References 30 publications

Physiochemical properties and resorption progress of porcine skin-derived collagen membranes: <i>In vitro</i> and <i>in vivo</i> analysis

Physiochemical properties and resorption progress of porcine skin-derived collagen membranes: <i>In vitro</i> and <i>in vivo</i> analysis

Preparation and evaluation of a novel pADM-derived micro- and nano electrospun collagen membrane

Biomedical Applications of Collagen/Gelatin

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