Syndecan-4 Binding to the High Affinity Heparin-Binding Domain of Fibronectin Drives Focal Adhesion Formation in Fibroblasts

Woods, Anne; Longley, Robert L.; Tumova, Sarka; Couchman, John R.

doi:10.1006/abbi.1999.1607

Cited by 207 publications

(204 citation statements)

References 32 publications

Supporting

Mentioning

191

Contrasting

Unclassified

Order By: Relevance

“…In line with previous work [21,[27][28][29], vinculin confocal immunofluorescence revealed fewer and smaller focal adhesions in fibroblasts from syndecan-4 -/-mice when compared to those from WT mice (Supplemental Figure S4) which may imply impaired focal adhesion signaling in cardiac fibroblasts from syndecan-4 -/-mice.…”

Section: Syndecan-4 and Calcineurin Co-localize In Cardiac Fibroblastssupporting

confidence: 90%

Syndecan-4 signaling via NFAT regulates extracellular matrix production and cardiac myofibroblast differentiation in response to mechanical stress

Herum

Lunde

Skrbic

et al. 2013

Journal of Molecular and Cellular Cardiology

113

101

View full text Add to dashboard Cite

Section: Syndecan-4 and Calcineurin Co-localize In Cardiac Fibroblastssupporting

confidence: 90%

Syndecan-4 signaling via NFAT regulates extracellular matrix production and cardiac myofibroblast differentiation in response to mechanical stress

Herum

Lunde

Skrbic

et al. 2013

Journal of Molecular and Cellular Cardiology

113

101

View full text Add to dashboard Cite

“…Syndecan-4 is involved in cytoskeletal reorganization, assembly of stress fibers, and FA formation. In fact, it has been shown to be required for FA formation in addition to integrins (Saoncella et al, 1999;Woods et al, 2000). Recruitment of syndecan-4 to FAs is dependent on PKC activation, and multimerization of the cytoplasmic domain of syndecan-4 is required for kinase binding and activation (Baciu and Goetinck, 1995).…”

Section: Cellular Mechanotransductionmentioning

confidence: 99%

Mechanoregulation of gene expression in fibroblasts

Wang

Thampatty

Lin

et al. 2007

Gene

225

187

View full text Add to dashboard Cite

Mechanical loads placed on connective tissues alter gene expression in fibroblasts through mechanotransduction mechanisms by which cells convert mechanical signals into cellular biological events, such as gene expression of extracellular matrix components (e.g., collagen). This mechanical regulation of ECM gene expression affords maintenance of connective tissue homeostasis. However, mechanical loads can also interfere with homeostatic cellular gene expression and consequently cause the pathogenesis of connective tissue diseases such as tendinopathy and osteoarthritis. Therefore, the regulation of gene expression by mechanical loads is closely related to connective tissue physiology and pathology. This article reviews the effects of various mechanical loading conditions on gene regulation in fibroblasts and discusses several mechanotransduction mechanisms. Future research directions in mechanoregulation of gene expression are also suggested.

show abstract

“…Activation of PKCα by syndecan-4 is a required step in the signaling pathway of FGF (31). The binding of syndecan-4 to fibronectin regulates PKC (54,55) and rho activity (68), which is important for the α5β1 dependent assembly of focal adhesion structures and actin stress fibers (90).…”

Section: Discussionmentioning

confidence: 99%

“…The interaction of syndecans with matrix molecules is thought to activate signaling pathways which result in changes in cell adhesion, morphology, and fibronectin matrix assembly (10,65,89). Syndecan-4 binds to fibronectin and activates signaling pathways important in the assembly of focal adhesions and actin stress fibers (46,68,90). Syndecan-1 binds to thrombospondin and triggers the formation of fascin containing microspikes (2).…”

Section: Introductionmentioning

confidence: 99%

Vitronectin's Basic Domain is a Syndecan Ligand which Functionsin transto Regulate Vitronectin Turnover

Wilkins-Port

Sanderson²,

Tominna-Sebald³

et al. 2003

Cell Communication & Adhesion

View full text Add to dashboard Cite

During the process of tissue remodeling, vitronectin (Vn) is deposited in the extracellular matrix where it plays a key role in the regulation of pericellular proteolysis and cell motility. In previous studies we have shown that extracellular levels of vitronectin are controlled by receptormediated endocytosis and that this process is dependent upon vitronectin binding to sulfated proteoglycans. We have now identified vitronectin's 12 amino acid "basic domain" which is contained within the larger 40 amino acid heparin binding domain, as a syndecan binding site. Recombinant vitronectins representing wild type vitronectin (rVn) and vitronectin with the basic domain deleted (rVn 347-358) were prepared in a baculoviral expression system. The rVn as well as a glutathione S-transferase (GST) fusion protein, consisting of vitronectin's 40 amino acid heparin binding domain (GST-VnHBD), exhibited dose dependent binding to HT-1080 cell surfaces, which was attenuated following deletion of the basic domain. In addition, GST-VnHBD supported both HT-1080 and dermal fibroblast cell adhesion, which was also dependent upon the basic domain. Similarly, ARH-77 cells transfected with syndecans -1, -2, or -4, but not Glypican-1, adhered to GST-VnHBD coated wells, while adhesion of these same cells was lost following deletion of the basic domain. HT-1080 cells were unable to degrade rVn 347-358. Degradation of rVn 347-358 was completely recovered in the presence of GST-VnHBD but not in the presence of GST-VnHBD 347-358. These results indicate that turnover of soluble vitronectin requires ligation of vitronectin's basic domain and that this binding event can work in trans to regulate vitronectin degradation.

show abstract

Syndecan-4 Binding to the High Affinity Heparin-Binding Domain of Fibronectin Drives Focal Adhesion Formation in Fibroblasts

Cited by 207 publications

References 32 publications

Syndecan-4 signaling via NFAT regulates extracellular matrix production and cardiac myofibroblast differentiation in response to mechanical stress

Syndecan-4 signaling via NFAT regulates extracellular matrix production and cardiac myofibroblast differentiation in response to mechanical stress

Mechanoregulation of gene expression in fibroblasts

Vitronectin's Basic Domain is a Syndecan Ligand which Functionsin transto Regulate Vitronectin Turnover

Contact Info

Product

Resources

About