Sympatric occurrence and population dynamics of Scylla spp. in equatorial climate: Effects of rainfall, temperature and lunar phase

Fazhan, Hanafiah; Waiho, Khor; Azri, Mohammad Farhan Darin; Al-Hafiz, Ismail; Norfaizza, Wan Ibrahim Wan; Megat, Fadhlul Hazmi; Jasmani, Safiah; Ma, Hongyu

doi:10.1016/j.ecss.2017.09.022

Cited by 28 publications

(32 citation statements)

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“…In our previous study, the number of female S. paramamosain at two-month old (older than C2) was also lower than males, but the sex ratio significantly skewed towards females from three-month old to four-month old (unpublished data). The variation of the sex ratio during the process of growth and development could be due to the known aggressive and cannibalistic behavior of Scylla spp., and the growing competition for food and spouses, especially for male crabs [33], which leads to injury and death of males.…”

Section: Discussionmentioning

confidence: 99%

“…Mud crabs ( Scylla spp.) are highly valuable commercial aquaculture species and fishery resources in the Indo-West Pacific region, such as China [29], Thailand [30], Vietnam [31], Philippines [32] and Malaysia [33]. The aquaculture output of mud crab in China reached approximately 148,977 tons, the top among all marine commercial crabs [34].…”

Section: Introductionmentioning

confidence: 99%

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Female-specific SNP markers provide insights into a WZ/ZZ sex determination system for mud crabs Scylla paramamosain, S. tranquebarica and S. serrata with a rapid method for genetic sex identification

Shi

Waiho

et al. 2018

BMC Genomics

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BackgroundMud crabs, Scylla spp., are commercially important large-size marine crustaceans in the Indo-West Pacific region. As females have the higher growth rate and economic value, the production of all female stocks is extremely essential in aquaculture. However, the sex determination mechanism is still unclear. Development of sex-specific genetic markers based on next-generation sequencing proved to be an effective tool for discovering sex determination system in various animals.ResultsRestriction-site associated DNA sequencing (RAD-seq) was employed to isolate sex-specific SNP markers for S. paramamosain. A total of 335.6 million raw reads were obtained from 20 individuals, of which 204.7 million were from 10 females and 130.9 million from 10 males. After sequence assembly and female-male comparison, 20 SNP markers were identified to be sex-specific. Furthermore, ten SNPs in a short sequence (285 bp) were confirmed heterozygous in females and homozygous in males in a large population by PCR amplification and sequencing. Subsequently, a female-specific primer was successfully designed according to the female-specific nucleotide which could amplify an expected band from females but not from males. Thus, a rapid and effective method for molecular sexing in S. paramamosain was developed, meanwhile, this method could successfully identify the sex of S. tranquebarica and S. serrata. Finally, nine and four female-specific SNP markers were detected in S. tranquebarica and S. serrata, respectively.ConclusionsSex-specific SNP markers were firstly identified in crab species and showed female heterogamety and male homogamety, which provided strong genetic evidence for a WZ/ZZ sex determination system in mud crabs S. paramamosain, S. tranquebarica and S. serrata. These findings will lay a solid foundation for the study of sex determination mechanism, sex chromosome evolution, and the development of mono-sex population in crustaceans.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5380-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Female-specific SNP markers provide insights into a WZ/ZZ sex determination system for mud crabs Scylla paramamosain, S. tranquebarica and S. serrata with a rapid method for genetic sex identification

Shi

Waiho

et al. 2018

BMC Genomics

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“…Currently, crab culture mainly relies on wild-caught seeds. However, this unregulated crab fishing practice, coupled with various anthropogenic factors, such as overfishing and environmental deterioration, results in a rapid decline in numbers of many economically important crab species 15 , 16 . Portunus sanguinolentus is generally known as the three-spot swimming crab due to the presence of three red to maroon prominent spots on the posterior part of its carapace 17 .…”

Section: Background and Summarymentioning

confidence: 99%

Transcriptome sequencing and molecular markers discovery in the gonads of Portunus sanguinolentus

Zhang

Miao

et al. 2018

Sci Data

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Crab culture has gained prominence in the last decade due to the large global market demand for live crabs and crab products. Portunus sanguinolentus is one of the economically important crab species in the Indo-Pacific region, with distinct differences in growth and size between male and female crabs, thus, leading to huge difference in their market values. The culture of P. sanguinolentus is still in its infancy, with crab supplies heavily dependent on wild catch. In order to unravel the molecular differences between male and female crabs, we generated a comprehensive transcriptomic dataset for P. sanguinolentus by sequencing the gonads of both sexes using the Illumina Hiseq 2500 system. Transcriptomes were assembled using Trinity de novo assembly followed by annotation. This transcriptomic data set for P. sanguinolentus would serve as an important reference data for genomic and genetic studies in this crab and related species.

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“…Apart from morphological variations, variation in body size is also obvious among Scylla species. S. serrata is the largest, followed by S. tranquebarica and S. paramamosain (Ogawa et al, 2012;Fazhan et al, 2017a). S. olivacea is considered as the smallest among the four species (Waiho, Fazhan & Ikhwanuddin, 2016b;Fazhan et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%

“…S. serrata is the largest, followed by S. tranquebarica and S. paramamosain (Ogawa et al, 2012;Fazhan et al, 2017a). S. olivacea is considered as the smallest among the four species (Waiho, Fazhan & Ikhwanuddin, 2016b;Fazhan et al, 2017a). However, due to the acclimatization to their natural habitat (upper river mouths with lower salinity and constant exposure to intertidal currents; , S. olivacea are the sturdiest.…”

Section: Introductionmentioning

confidence: 99%

Morphological descriptions and morphometric discriminant function analysis reveal an additional four groups of Scylla spp

Fazhan

Waiho

Quinitio

et al. 2020

PeerJ

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There are four species of mud crabs within the genus Scylla, and most of them live sympatrically in the equatorial region. Apart from a report in Japan about the finding of a natural Scylla hybrid more than a decade ago after the division of genus Scylla into four species by Keenan, Davie & Mann (1998), no subsequent sighting was found. Thus, this study investigates the possible natural occurrence of potential hybridization among Scylla species in the wild. A total of 76,211 individuals from mud crab landing sites around the Malacca Straits, South China Sea and Sulu Sea were screened. In addition to the four-purebred species, four groups (SH 1, n = 2, 627; SH 2, n = 136; SH 3, n = 1; SH 4, n = 2) with intermediate characteristics were found, mostly at Sulu Sea. Discriminant Function Analysis revealed that all Scylla species, including SH 1 - 4, are distinguishable via their morphometric ratios. The most powerful discriminant ratios for each character and the top five discriminant ratios of males and females were suggested. The carapace width of SH 1 males and females were significantly smaller than pure species. Based on the discriminant ratios and the description of morphological characters, we hypothesize that the additional four groups of Scylla with intermediate characteristics could be presumed hybrids. Future work at the molecular level is urgently needed to validate this postulate.

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Sympatric occurrence and population dynamics of Scylla spp. in equatorial climate: Effects of rainfall, temperature and lunar phase

Cited by 28 publications

References 43 publications

Female-specific SNP markers provide insights into a WZ/ZZ sex determination system for mud crabs Scylla paramamosain, S. tranquebarica and S. serrata with a rapid method for genetic sex identification

Female-specific SNP markers provide insights into a WZ/ZZ sex determination system for mud crabs Scylla paramamosain, S. tranquebarica and S. serrata with a rapid method for genetic sex identification

Transcriptome sequencing and molecular markers discovery in the gonads of Portunus sanguinolentus

Morphological descriptions and morphometric discriminant function analysis reveal an additional four groups of Scylla spp

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