Symbiotic essential amino acids provisioning in the American cockroach,<i>Periplaneta americana</i>(Linnaeus) under various dietary conditions

Ayayee, Paul A.; Larsen, Thomas; Sabree, Zakee L.

doi:10.7717/peerj.2046

Cited by 26 publications

(24 citation statements)

References 35 publications

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“…The ability of some bacteria to provide essentials amino acids is well documented in insects (Douglas, ), however no cases of nutritional symbiosis have been recorded so far among lepidopterans (McCutcheon & Moran, ; Ayayee et al ., ). The results achieved in this study may pave the way for future studies to investigate the role of Burkholderia and other bacteria of the P. interpunctella microbiota in supporting the host development.…”

Section: Discussionmentioning

confidence: 97%

Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

et al. 2017

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Diversity and composition of lepidopteran microbiotas are poorly investigated, especially across the different developmental stages. To improve this knowledge, we characterize the microbiota among different developmental stages of the Indian meal moth, Plodia interpunctella, which is considered one of the major pest of commodities worldwide. Using culture-independent approach based on Illumina 16S rRNA gene sequencing we characterized the microbiota of four developmental stages: eggs, first-, and last-instar larvae, and adult. A total of 1022 bacterial OTUs were obtained, showing a quite diversified microbiota associated to all the analyzed stages. The microbiotas associated with P. interpunctella resulted almost constant throughout the developmental stages, with approximately 77% of bacterial OTUs belonging to the phylum of Proteobacteria. The dominant bacterial genus is represented by Burkholderia (∼64%), followed by Propionibacterium, Delftia, Pseudomonas, and Stenotrophomonas. A core bacterial community, composed of 139 OTUs, was detected in all the developmental stages, among which 112 OTUs were assigned to the genus Burkholderia. A phylogenetic reconstruction, based on the 16S rRNA, revealed that our Burkholderia OTUs clustered with Burkholderia cepacia complex, in the same group of those isolated from the hemipterans Gossyparia spuria and Acanthococcus aceris. The functional profiling, predicted on the base of the bacterial 16S rRNA, indicates differences in the metabolic pathways related to metabolism of amino acids between preimaginal and adult stages. We can hypothesize that bacteria may support the insect host during preimaginal stages.

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Section: Discussionmentioning

confidence: 97%

Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

et al. 2017

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“…An important general fitness contribution of microbiomes to their hosts is carrying out metabolic processes that the animal or plant cannot carry out by themselves [ 59 ]. There are many examples: nitrogen fixation in legumes [ 60 ], cellulose degradation in ruminants [ 61 ], termites [ 62 ] and cockroaches [ 63 ], essential amino acid synthesis in insects [ 64 ], photosynthesis by microalgae in corals, mollusks and sponges [ 65 ], and oxidation of inorganic compounds [ 66 ] and hydrocarbons in deep-sea invertebrates [ 67 ].…”

Section: Interactive Fitness In Holobiontsmentioning

confidence: 99%

The hologenome concept of evolution after 10 years

2018

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The holobiont (host with its endocellular and extracellular microbiome) can function as a distinct biological entity, an additional organismal level to the ones previously considered, on which natural selection operates. The holobiont can function as a whole: anatomically, metabolically, immunologically, developmentally, and during evolution. Consideration of the holobiont with its hologenome as an independent level of selection in evolution has led to a better understanding of underappreciated modes of genetic variation and evolution. The hologenome is comprised of two complimentary parts: host and microbiome genomes. Changes in either genome can result in variations that can be selected for or against. The host genome is highly conserved, and genetic changes within it occur slowly, whereas the microbiome genome is dynamic and can change rapidly in response to the environment by increasing or reducing particular microbes, by acquisition of novel microbes, by horizontal gene transfer, and by mutation. Recent experiments showing that microbiota can play an initial role in speciation have been suggested as an additional mode of enhancing evolution. Some of the genetic variations can be transferred to offspring by a variety of mechanisms. Strain-specific DNA analysis has shown that at least some of the microbiota can be maintained across hundreds of thousands of host generations, implying the existence of a microbial core. We argue that rapid changes in the microbiome genome could allow holobionts to adapt and survive under changing environmental conditions thus providing the time necessary for the host genome to adapt and evolve. As Darwin wrote, “It is not the strongest of the species that survives but the most adaptable”.

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“…Regarding AA ESS , recent studies on invertebrates [66][67][68] and vertebrates [35,69] show that dietary protein is not the sole source of AA ESS used to build and maintain animal tissues. Because eukaryotic consumers cannot synthesize AA ESS de novo, they should directly route dietary AA ESS into their tissues, resulting in ∆ 13 C C-D values of~0‰ ; however, this fundamental expectation has not always been met in controlled experiments.…”

Section: Csia Applications To Datementioning

confidence: 99%

A Guide to Using Compound-Specific Stable Isotope Analysis to Study the Fates of Molecules in Organisms and Ecosystems

et al. 2019

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The measurement of stable isotopes in ‘bulk’ animal and plant tissues (e.g., muscle or leaf) has become an important tool for studies of functional diversity from organismal to continental scales. In consumers, isotope values reflect their diet, trophic position, physiological state, and geographic location. However, interpretation of bulk tissue isotope values can be confounded by variation in primary producer baseline values and by overlapping values among potential food items. To resolve these issues, biologists increasingly use compound-specific isotope analysis (CSIA), in which the isotope values of monomers that constitute a macromolecule (e.g., amino acids in protein) are measured. In this review, we provide the theoretical underpinnings for CSIA, summarize its methodology and recent applications, and identify future research directions. The key principle is that some monomers are reliably routed directly from the diet into animal tissue, whereas others are biochemically transformed during assimilation. As a result, CSIA of consumer tissue simultaneously provides information about an animal’s nutrient sources (e.g., food items or contributions from gut microbes) and its physiology (e.g., nitrogen excretion mode). In combination, these data clarify many of the confounding issues in bulk analysis and enable novel precision for tracing nutrient and energy flow within and among organisms and ecosystems.

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Symbiotic essential amino acids provisioning in the American cockroach,Periplaneta americana(Linnaeus) under various dietary conditions

Cited by 26 publications

References 35 publications

Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

The hologenome concept of evolution after 10 years

A Guide to Using Compound-Specific Stable Isotope Analysis to Study the Fates of Molecules in Organisms and Ecosystems

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