Sustained-release of protein from biodegradable sericin film, gel and sponge

Nishida, Ayumu; Yamada, Masaki; Kanazawa, Takanori; Takashima, Yuuki; Ouchi, Kiyohisa; Okada, Hiroaki

doi:10.1016/j.ijpharm.2011.01.006

Cited by 68 publications

(55 citation statements)

References 44 publications

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“…We previous studied the molecular weight of SC after heating under above conditions using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and there are three kinds of molecular weight of intact SC proteins (180, 250, 400 kDa), which is able to form hydrogel with water in the soluble components. 3,4) Next, insoluble substances were separated by centrifugation, and the soluble components were collected and used as 2% SC solution. Then, this solution was combined with either AD or siRNA solution, and stored overnight at 4°C to produce a 1% SC hydrogel containing either AD or siRNA.…”

Section: Materials and Animalsmentioning

confidence: 99%

“…We have previously succeeded in improving sustained release by integrating albumin and fibroblast growth factor-2 (FGF-2) using sericin (SC), a biodegradable and biocompatible silk protein, thereby forming a hydrogel without the use of a crosslinking reagent. [3][4][5] Additionally, the sericin has biodegradability, biocompatibility, low-immunogenicity and nontoxicity. 3,6) We also developed a hydrogel formulation including RelA-targeting small interfering RNA (siRNA) (siRelA).…”

mentioning

confidence: 99%

“…[3][4][5] Additionally, the sericin has biodegradability, biocompatibility, low-immunogenicity and nontoxicity. 3,6) We also developed a hydrogel formulation including RelA-targeting small interfering RNA (siRNA) (siRelA). The siRelA retention was improved at the administration site after topical application in a mouse model of atopic dermatitis, and significant improvement of symptoms was observed upon application of the SC hydrogel in comparison with application of siRelA alone.…”

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confidence: 99%

See 2 more Smart Citations

Intra-articular Retention and Anti-arthritic Effects in Collagen-Induced Arthritis Model Mice by Injectable Small Interfering RNA Containing Hydrogel

Kanazawa

Tamano

Sogabe

et al. 2017

Biological & Pharmaceutical Bulletin

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Small interfering RNAs (siRNAs) are expected to offer a means of treating rheumatoid arthritis (RA) because they allow the specific silencing of genes related to RA pathogenesis. In our previous study, we reported that the siRNA targeted against RelA (anti-RelA siRNA), an important nuclear factor-kappaB (NF-κB) subdomain, was an effective therapeutic in atopic dermatitis and RA model animals. In this study, to develop an intra-articular injectable gel formulation against RA, we prepared a hydrogel that contains anti-RelA siRNA, and determined the in vitro release profile (%) and in vivo intra-articular retention of fluorescence-labeled model siRNA, and the anti-arthritic effects of the anti-RelA siRelA containing hydrogel in RA model mice. We selected the silk protein, sericin (SC), as an aqueous gel base, as it is a biocompatible and useful for forming hydrogels without a cross-linker. We showed that fluorescence-labeled model siRNA was continuously released from SC hydrogel in vitro, and retained in the knee joint of rats after injection of siRNA hydrogel. In addition, the knee joint thickness, clinical severity and incidence (%) in collagen-induced arthritis (CIA) mice as RA model treated with anti-RelA siRNA containing hydrogel were more improved than untreated, anti-RelA siRNA solution and negative control siRNA containing hydrogel group. Therefore, the intra-articular injectable sericin hydrogel formulation containing of anti-RelA siRNA could be a great potential therapeutic in rheumatoid arthritis.

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Section: Materials and Animalsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Intra-articular Retention and Anti-arthritic Effects in Collagen-Induced Arthritis Model Mice by Injectable Small Interfering RNA Containing Hydrogel

Kanazawa

Tamano

Sogabe

et al. 2017

Biological & Pharmaceutical Bulletin

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“…Biodegradable sericin films with sustained-release of protein are reported [44]. Membranes of A. mylitta cocoons and gland sericin are evaluated for cell growth [22,24,45].…”

Section: (B) Filmsmentioning

confidence: 99%

Silk Protein Sericin: Promising Biopolymer for Biological and Biomedical Applications

Nayak

Kundu

2016

Biomaterials From Nature for Advanced Devices and Therapies

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“…Film has been noted as a dosage form in the fields of medicine and cosmetics [1,2]. The film dosage form (FD) adheres to a surface when a little liquid is present on it, and then the drug or another active compound incorporated in the film is released.…”

Section: Introductionmentioning

confidence: 99%