Sustained perfusion of revascularized bioengineered livers heterotopically transplanted into immunosuppressed pigs

Shaheen, Mohammed F; Joo, Dong Jin; Ross, Jeffrey; Anderson, Brett D.; Chen, Harvey S.; Huebert, Robert C.; Li, Yang; Amiot, Bruce; Young, Alison; Zlochiver, Sharon; Nelson, Erek; Mounajjed, Taofic; Dietz, Allan B.; Michalak, Gregory J.; Steiner, Benjamin G.; Davidow, Dominique S.; Paradise, Christopher R.; Wijnen, André J. van; Shah, Vijay H.; Liu, Mengfei; Nyberg, Scott L.

doi:10.1038/s41551-019-0460-x

Cited by 51 publications

(81 citation statements)

References 43 publications

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“…In that way, the oxygen and nutrition would flow into an open capillary in liver organoids and connect with outside vessels. Recently, sustained perfusion of revascularized liver scaffolds has been achieved by incorporating HUVECs into decellularized porcine livers ( Shaheen et al, 2020 ). As looking forward, we would expect transplantable livers at the centimeter scale with direct surgical anastomosis to patient vessels and establishing immediate blood perfusion ( Figure 3 ).…”

Section: Perspectivesmentioning

confidence: 99%

Progress in human liver organoids

Hui

2020

Journal of Molecular Cell Biology

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Understanding the development, regeneration, and disorders of the liver is the major goal in liver biology. Current mechanistic knowledge of human livers has been largely derived from mouse models and cell lines, which fall short in recapitulating the features of human liver cells or the structures and functions of human livers. Organoids as an in vitro system hold the promise to generate organ-like tissues in a dish. Recent advances in human liver organoids also facilitate the understanding of the biology and diseases in this complex organ. Here we review the progress in human liver organoids, mainly focusing on the methods to generate liver organoids, their applications, and possible future directions.

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Section: Perspectivesmentioning

confidence: 99%

Progress in human liver organoids

Hui

2020

Journal of Molecular Cell Biology

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“…Recent progress in stem cell research and organ bioengineering have generated a viable pathway toward these goals. ( 2 ) Induced pluripotent stem cells (iPSC) have the capacity to differentiate into the most mature liver cell types, making them ideal candidates for cell‐based regenerative medicine for liver disease. ( 3 ) Furthermore, the ability to use a patient’s own cells to generate the iPSC provides for a personalized approach.…”

Section: Figurementioning

confidence: 99%

“…It is unknown how feasible it will be to scale the approach to larger organs, larger cell masses, and other species. Similar decellularization/recellularization technology has been used recently to demonstrate sustained perfusion of bioengineered livers in a large animal model, ( 2 ) but these efforts initially focused on revascularization with endothelial cells. Challenges remain with respect to cell types and cell seeding protocols, ex vivo maintenance of recellularized organs, preventing vascular thrombotic events, and recapitulating the intricate liver microstructures, such as a fenestrated sinusoidal endothelium and bile canaliculi.…”

Section: Figmentioning

confidence: 99%

Bioengineered Mini Human Livers for Transplantation

2020

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“… 11 During the past decade different human and animal organs and tissues have been utilized as dECM scaffolds, proving their potential application in tissue engineering ( Table 1 ). The progression of decellularization techniques has been advancing for different tissue and organs like heart, 12 – 15 liver, 16 , 17 lung, 18 – 21 kidney, 22 , 23 cornea, 24 , 25 skin, 26 , 27 brain, 28 adipose tissue. 29 …”

Section: Introductionmentioning

confidence: 99%

Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications

et al. 2021

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Due to the current lack of innovative and effective therapeutic approaches, tissue engineering (TE) has attracted much attention during the last decades providing new hopes for the treatment of several degenerative disorders. Tissue engineering is a complex procedure, which includes processes of decellularization and recellularization of biological tissues or functionalization of artificial scaffolds by active cells. In this review, we have first discussed those conventional steps, which have led to great advancements during the last several years. Moreover, we have paid special attention to the new methods of post-decellularization that can significantly ameliorate the efficiency of decellularized cartilage extracellular matrix (ECM) for the treatment of osteoarthritis (OA). We propose a series of post-decellularization procedures to overcome the current shortcomings such as low mechanical strength and poor bioactivity to improve decellularized ECM scaffold towards much more efficient and higher integration.

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Sustained perfusion of revascularized bioengineered livers heterotopically transplanted into immunosuppressed pigs

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Cited by 51 publications

References 43 publications

Progress in human liver organoids

Progress in human liver organoids

Bioengineered Mini Human Livers for Transplantation

Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications

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