Susceptibility tests of oropharyngeal Candida albicans from Egyptian patients to fluconazole determined by three methods

Abstract

Candida albicans frequently cause oropharyngeal candidiasis in immunocompromised patients. As some of these isolates show resistance against azoles, the clinician is wary of initiating therapy with fluconazole (FZ) until a final susceptibility report is generated. We aimed to evaluate the efficacy of rapid flow cytometry (FCM) and disc diffusion (DD) methods in comparison to reference microdilution (MD) of Clinical and Laboratory Standards Institute (CLSI) method for FZ. Thirty seven Candida albicans isolates were tested by the three methods. By both MD and FCM, 26/37 (70.3%) were sensitive with minimal inhibitory concentration (MIC) ≤ 8μg/ml, 5/37 (13.5%) were susceptible dose dependant (S-DD) with MIC 16-32 μg/ml and 6/37 (16.2%) were resistant with MIC ≥64μg/ml. More than 92% of isolates susceptible to FZ by the MD were susceptible by the DD methods with good agreement (81.08%, P = 0.000). However, 4/5 isolates diagnosed as S-DD by MD were resistant by DD. Interestingly, the MIC by FCM at 4 h showed excellent agreement (95.59%, P = 0.000) to that obtained by MD method at 24 h. Overall, FCM antifungal susceptibility testing provided rapid, reproducible results that are valuable alternative to MD. The DD test is recommended as a simple and reliable screening test for the detection of susceptible Candida albicans isolates to FZ

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

“…Culturally and morphologically characterized yeast isolates were resistant to majority of the antibacterial drugs, because of their cell wall composition which differs with prokaryotes (Czerucka et al 2007) and most of the isolates were sensitive towards maximum antifungal drugs (Mashad et al 2012). …”

“…Ltd., India) were placed on yeast inoculated YPD medium plates and incubated for 48 h at 30 °C. After incubation drug sensitivity of the yeast isolates was evaluated by measuring the zone of inhibition (Mashad et al 2012). …”

Chelating Behaviour of Dihydrothieno[3,4, d] Pyridazine Derivatives with Some Lanthanide and Actinide Metals

“…Isolated colonies of each organism that might he playing a pathogenic role should be selected from primary agar plates and tested for susceptibility by disc diffusion method 39 , results in good batch-to-batch reproducibility. Disc diffusion method for filamentous fungi tested by using approved standard method (M38-A) developed by, evaluating the susceptibilities of filamentous fungi to antifungal agents 40 . Disc diffusion method for yeasts developed by using approved standard method (M44-P) 41 .…”

Novel Simple Cyanine, Carbocyanine, and Dicarbocyanine Dyes: Synthesis, Characterization and Application on Polyester Fabric

“…The data obtained are expressed as size (mm) of inhibition zone. Diameter of the inhibition zone were high (22-18 mm), moderate (17-12 mm), low (11-1 mm), no response(-) (Ballatore et al, 2012;King et al,2010;El-Mashad et al,2012;Mohamed et al, 2014). Studying the antimicrobial(antibacterial, antifungal)activity evaluation against some bacterial and fungi strains have a great practical value in cyanine dyes because of the uses and applications of these dyes as bactericidal and fungicidal in pharmaceutical industry.…”