Susceptibility of oral Candida species to calcium hydroxide in vitro

Waltimo, Tuomas; Sirén, E. K.; Ørstavik, Dag; Haapasalo, Markus

doi:10.1046/j.1365-2591.1999.00195.x

Cited by 131 publications

(109 citation statements)

References 19 publications

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“…After direct contact of the yeasts with the solution, the yeast viability was investigated by means of culture and vital staining methods. The mycological culture showed complete inhibition of yeasts only after 48 h of incubation, revealing high resistance of C. albicans strains to calcium hydroxide, as reported previously (12,13). On the other hand, the vital staining method revealed total viability at 6 and 12 h after incubation.…”

Section: Discussionsupporting

confidence: 84%

“…In addition to the capacity of invasion, C. albicans has been found to be resistant to some intracanal medications after direct contact (12). Considering the increasing interest for the role of fungi in endodontic infections, secondary or persistent ones, and endodontic treatment failure as well as the recent reports on fungal resistance against commonly used intracanal medications, this study evaluated the in vitro susceptibility of oral C. albicans samples to different pH values and to saturated calcium hydroxide aqueous solution.…”

Section: In Vitro Susceptibility Of Oral Candida Albicansmentioning

confidence: 99%

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In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution

et al. 2012

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Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.

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Section: Discussionsupporting

confidence: 84%

Section: In Vitro Susceptibility Of Oral Candida Albicansmentioning

confidence: 99%

In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution

et al. 2012

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“…that were found to be resistant to CaOH 2 [22]. Therefore, we decided to use alternative intracanal medicaments in our study (Ledermix and TAP).…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of postoperative pain intensity after single-visit and multiple-visit retreatment cases: a prospective randomized clinical trial

Uyan

Olcay

Özcan

2018

BDS

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26 ResumoObjetivo: Este estudo foi projetado para avaliar a dor pós-operatória após o retratamento endodôntico. Material e Métodos: Foram incluídos dentes molares e pré-molares multirradiculados e assintomáticos que necessitavam de retratamento com lesões periapicais de 2-5 mm. Setenta e oito dentes foram distribuídos aleatoriamente em quatro grupos (n = 20): sessão única (controle, grupo I), Ledermix (grupo II), metronidazol, ciprofloxacina, mistura de minociclina (grupo III), hidróxido de cálcio (grupo IV). A dor pós-operatória foi registrada usando um VAS às 6, 12, 24 e 48 h após o retratamento. A análise estatística foi realizada utilizando ANOVA de dois fatores para medidas repetidas e teste de Tukey. Resultados: Ocorreu dor leve em 67,5%, moderada em 30% e agudizações 2,5%, e houve diferença significativa entre os grupos (p <0,01). Foi observada dor pós-operatória significativamente menor nos grupos TAP e hidróxido de cálcio (p <0,05). Nos intervalos de 6, 12 e 24 h, houve diferença significativa nos níveis de dor (p <0,05). Conclusão: TAP e hidróxido de cálcio são eficazes para reduzir a dor pós-operatória após o retratamento. ABsTRACTObjectives: This study was designed to evaluate postoperative pain after endodontic retreatment. Material and Methods: Asymptomatic, multirooted molar & premolar teeth requiring retreatment with 2-5 mm periapical lesions were included. Seventy-eight teeth were randomly placed in four groups (n=20): single-visit (control, group 1), Ledermix (group 2), metronidazole, ciprofloxacin, minocycline mixture (group 3), calcium hydroxide (group 4). The postoperative pain was recorded using a VAS at 6, 12, 24, and 48 h after retreatment. Statistical evaluation was performed using TwoWay Repeated Measures ANOVA and Tukey test. Results: Mild pain occurred in 67.5%, moderate in 30%, and flare-ups in 2.5%, and there was a significant difference between the groups (p<0.01). Significantly lower postoperative pain was observed in TAP and CaOH2 groups (p<0.05). In the 6, 12, and 24 h intervals, there was a significant difference in the pain levels (p<0.05). Conclusion: TAP and CaOH 2 are effective for reducing postoperative pain after retreatment. O R I G I N A L A R T I C L EComparative evaluation of postoperative pain intensity after single-visit and multiple-visit retreatment cases: a prospective randomized clinical trial Avaliação comparativa da intensidade da dor pos-operatoria após casos de retratamento em sessao unica ou multipla: estudo clínico randomizado prospectivo PAlAvRAs-ChAveMedicamentos intracanais; Múltiplas sessões; Dor pós-operatória; Retratamento; Sessão única.

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“…Both of them can be easily grown under aerobic conditions and have shown resistance to substances used in endodontic treatment, including CH (12). Indeed, such a resistance to CH has been one factor that has prompted researchers to propose association of CH with other substances, such as CPMC and Cx, so as to increase the spectrum of antimicrobial activity to include these resistant species (13)(14)(15).…”

Section: Discussionmentioning

confidence: 99%

Influence of serum and necrotic soft tissue on the antimicrobial effects of intracanal medicaments

et al. 2010

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The purpose of this study was to investigate the influence of serum and necrotic soft tissue on the antimicrobial activity of intracanal medicaments. The medicaments tested were: calcium hydroxyde/glycerin paste, calcium hydroxide/chlorhexidine paste, calcium hydroxide/camphorated paramonochlorophenol/glycerin paste, and chlorhexidine/zinc oxide paste. Survival of Enterococcus faecalis and Candida albicans exposed to the medicaments tested in the presence or absence of serum or necrotic tissue was monitored in three in vitro experiments where samples for culturing were taken at different time periods. The overall results demonstrated that the antimicrobial activity of all intracanal medicaments tested was slowed down in the presence of necrotic tissue. Calcium hydroxide pastes in glycerin or chlorhexidine were significantly affected by serum. Of the medicaments tested in this study, the least affected was the calcium hydroxide/camphorated paramonochlorophenol/glycerin paste.

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Susceptibility of oral Candida species to calcium hydroxide in vitro

Abstract: This study indicates that Candida spp. are resistant to calcium hydroxide in vitro, which may explain the isolation of yeasts from cases of persistent apical periodontitis.

Cited by 131 publications

References 19 publications

In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution

In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution

Comparative evaluation of postoperative pain intensity after single-visit and multiple-visit retreatment cases: a prospective randomized clinical trial

Influence of serum and necrotic soft tissue on the antimicrobial effects of intracanal medicaments

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