Survivorship and Egg Production of Phytophagous Pentatomids in Laboratory Rearing

Abstract

Survivorship and reproductive performance of the pentatomids Euschistus heros (F.) (EH), Nezara viridula (L.) (NV), and Dichelops melacanthus (Dallas) (DM) were tested in the laboratory. A mixture of natural foods (pods of green beans, Phaseolus vulgaris, raw shelled peanuts, Arachis hypogaea, and fruits of privet, Ligustrum lucidum, and 50 pairs/box (25 x 20 x 20 cm) were used, observed for 30 days, and replicated three times. Thirty days after emergence, mean female survivorship was 91% (EH), 60% (NV), and 30% (DM). More egg masses were deposited during 11-20 days after emergence, with mean number of 45.1 (EH), 5.3 (NV), and 11.8 (DM). These values were smaller during the irst 10 days (25.5, 2.1, and 4.7) and last 10 days (21-30 days) (39.4, 3.9, and 4.9), respectively. Mean maximum number of eggs/day was 489 (EH) on day 29, 474 (NV) on day 11, and 153 (DM) on day 14. Mean monthly fecundity (egg masses/box) was 985 (EH), 92 (NV), and 193 (DM), and mean number of eggs/ box was 8,480; 5,147, and 2,042.7, respectively.

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

“…Newly molted stinkbug females have undeveloped ovaries, and the ovary development seems to proceed at the same rate regardless of whether the female has mated or not (Fortes et al, 2011). The pre-mating and pre-oviposition periods seem to vary based on the species, with the pre-mating period lasting up to ten days with pre-oviposition periods of up to two weeks (Fortes et al, 2011;Silva et al, 2011). Similarly in WCR, the pre-oviposition period lasts between 12 and 15 days (Branson and Johnson, 1973;Hill, 1975).…”

“…Higher mortality was observed in females injected with brahma and mi-2 dsRNA compared to the other dsRNA applications ( Figure 6A). Stink bug females molt into adults with undeveloped ovaries, and under laboratory conditions the pre-oviposition period in E. heros lasts approximately 8 days (Silva et al, 2011). To allow for a preoviposition period, oviposition in E. heros was monitored after dsRNA injection and the eggs were collected on a daily basis after the negative control insects initiated oviposition.…”

Comparative biology of two congeneric stinkbugs, Chinavia impicticornis and C. ubica (Hemiptera: Pentatomidae)

“…However, even in this case, rearing conditions cannot be disregarded. In general, diet composition and environmental conditions influence insect performance (Parra, 2001;Cohen, 2004;Parra et al, 2012), particularly for the Pentatomidae family (Panizzi, 1997;Silva et al, 2008;Silva et al, 2011), in laboratory colonies. Since the optimal rearing conditions are not know for C. ubica and C. impicticornis, a negative influence of the procedures and environmental conditions adopted in the present work could still have had a significant influence on their development.…”

REPRODUÇÃO DE Edessa meditabunda (HEMIPTERA: PENTATOMIDAE) EM ALGODOEIRO

“…Além disso, a dieta padrão utilizada neste experimento é usualmente considerada testemunha em experimentos e para criação de pentatomídeos fitófagos em laboratório e é conhecida por permitir o completo desenvolvimento ninfal e a sobrevivência de percevejos (Fortes et al, 2006;Silva et al, 2011), resultando normalmente em um menor tempo de desenvolvimento das ninfas. Mesmo com o prolongamento no período de desenvolvimento das ninfas de E. meditabunda, o algodoeiro permitiu a sobrevivência destas, mostrando que 71,1% das ninfas alimentadas com algodoeiro conseguem atingir a fase adulta.…”