Survey of commercial Rhodiola products revealed species diversity and potential safety issues

Xin, Tianyi; Li, Xiaojin; Yao, Hui; Lin, Yulin; Ma, Xiaochong; Cheng, Ruiyang; Song, Jingyuan; Ni, Lulu; Fan, Congzhao; Chen, Shilin

doi:10.1038/srep08337

Cited by 86 publications

(79 citation statements)

References 24 publications

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“…Compared with other candidate DNA barcodes, namely, psbA-trnH, matK, rbcL, rpoC1, ycf5, and ITS, the ITS2 sequence represents the most suitable region for DNA barcoding applications to classify medicinal plant species [22]. Most medicinal plants such as Cornus officinalis, Menispermum dauricum, Rhodiola crenulata, and Selaginella tamariscina could be identified effectively based on ITS2 barcode [19,20,35,39]. In the present study, the ITS2 region was used to distinguish Shi-Liang tea species from closely related species.…”

Section: Application Of Its2 Sequences In Identification Of She Medicinementioning

confidence: 99%

“…DNA markers have become convenient tools for species identification and molecular systematics as a result of the advances in molecular technology [34][35][36]. However, ITS/ITS2 regions as universal barcodes present several problems and limitations, such as ITS/ITS2 showed poor PCR success in non-angiosperms [36].…”

Section: Application Of Its2 Sequences In Identification Of She Medicinementioning

confidence: 99%

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Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Zhou

et al. 2017

Applied Sciences

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Traditional She medicine is part of China's cultural heritage and has become remarkably popular worldwide. The Shi-Liang tea is made from the processed leaves of Chimonanthus salicifolius S. Y. Hu and Chimonanthus zhejiangensis M. C. Liu. To ensure the safety and efficacy of Shi-Liang tea, we used DNA barcoding based on the internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA to distinguish the original plant sources of Shi-Liang tea from closely related species. All 71 ITS2 sequences were aligned by Clustal-W, and genetic distances were computed using MEGA 6.0 according to the Kimura 2-parameter model. The results indicated that the sequence lengths of ITS2 regions of the original plants of Shi-Liang tea and closely related species ranged from 256 bp to 260 bp. Interspecific genetic distances ranged from 0 to 0.078. The neighbor-joining (NJ) tree showed that the original plants of Shi-Liang tea species can be easily differentiated from closely related species. Distinct molecular differences were found between the secondary structures of ITS2 sequences from Shi-Liang tea and closely related species. The results in the present investigation suggested that the ITS2 could be an effective DNA marker to identify the original plants of Shi-Liang tea and their closely related species. These DNA barcodes can greatly benefit the supervision of the commercial circulation of She medicine.

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Section: Application Of Its2 Sequences In Identification Of She Medicinementioning

confidence: 99%

Section: Application Of Its2 Sequences In Identification Of She Medicinementioning

confidence: 99%

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Zhou

et al. 2017

Applied Sciences

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“…The recently published results, along with the growing number of reports on the authenticity and quality control of R. rosea products (Xin, 2015;Booker et al 2015) are increasingly justifying the importance of in vitro systems optimization for reliable and homogenous plant material production. Iceland; this has been reviewed previously ).…”

Section: Phytochemical Composition Of Rhodiola Roseamentioning

confidence: 99%

Rhodiola rosea L.: from golden root to green cell factories

et al. 2016

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“…The use of authentic medicinal plant species is a fundamental requirement in herbal medicine [12]. Adulteration, substitution, and mislabelling may cause potential harm to patients [13][14][15][16][17]. Along with safety and toxicity, there is a need to develop methods of profiling medicinal plants for the purpose of authentication.…”

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Detection of Adulteration by Wedelia calendulacea in Eclipta alba through ISSR and RAPD Markers

Pendkar

Hegde

Nayak

et al. 2016

PMIO

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!In the crude herbal drug market, the highly medicinal Eclipta alba, popularly known as "Bhringaraja", is often adulterated with Wedelia calendulacea. To evaluate the ability of simple genetic fingerprinting methods to differentiate between the two species, Inter-Simple Sequence Repeat and Random Amplified Polymorphic DNA assays were performed on 30 individuals of E. alba and 4 individuals of W. calendulacea. Samples of W. calendulacea were found to cluster in clades that distinctly separated from E. alba and at least one band was identified for each of the two species that can serve as a marker for identification. Despite wide variations and a limited number of samples, phylogenetic analysis helped in differentiating the original from the adulterant plant. The study underscores the importance of simple DNAbased fingerprinting as a cost-effective method for the detection of adulteration in plants, particularly with respect to E. alba. Eclipta alba (L.) Hassk, commonly known as "Bhringaraja", is a medicinal herb belonging to the Asteraceae family that is traditionally used as a liver tonic and hair growth promoter in various traditional systems of medicine including Ayurveda. It is used in the treatment of spleen enlargements, uterine hemorrhages, skin diseases, and scorpion bites [1][2][3][4]. Other than the use of the whole plant juice as a powerful hepatoprotective and rejuvenative tonic, it is also used for the treatment of respiratory disorders, including coughs and asthma [5]. Wedelia calendulacea (L.) Less ("Pithabringi"), which belongs to the same family as E. alba, has a similar common name, and has been known for similar therapeutic uses [1,4]. It is often used as a substitute for the latter. It is interesting to note that wedelectone, a coumestan, is one of the major polyphenolic active constituents found in both species and it has been reported for activities against breast and prostate carcinomas [6,7]. Correct botanical identities of several crude drugs used in Ayurveda and in other traditional systems have not been properly established. These drugs have been categorized as "Sandigdha Dravya" or doubtful entities [8]. These are herbs with names that do not precisely indicate the exact botanical identity, but describe the therapeutic utility of the plant. The same is true with "Bhringaraja". Both E. alba and W. calendulacea are used under the name "Bhringaraja", often for treatment of the same ailment. However, it is reported that "Shweta Bhringaraja" -white-flowered herb (E. alba) -is superior in quality [9]. Reports of "E. alba" being adulterated with W. calendulacea are frequent [2,10,11]. Quality issues, including the genuineness of crude drugs are a major concern plaguing the herbal drug industry, particularly in India. The use of authentic medicinal plant species is a fundamental requirement in herbal medicine [12]. Adulteration, substitution, and mislabelling may cause potential harm to patients [13][14][15][16][17]. Along with safety and toxicity, there is a need to develop methods o...

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Survey of commercial Rhodiola products revealed species diversity and potential safety issues

Cited by 86 publications

References 24 publications

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Rhodiola rosea L.: from golden root to green cell factories

Detection of Adulteration by Wedelia calendulacea in Eclipta alba through ISSR and RAPD Markers

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