Survey of antibiotics residues in DDGS from 14 different states by LCMS

Olendorff, Samantha A; Chmielewska, Karolina; Tucker, Kevin R.

doi:10.1002/cche.10370

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Therefore, both the dry weight and activity of cells in the initial bioprocess inoculum should be considered [73]. Viral, bacteriophage, and bacterial contamination can severely impact on both mammalian and microbial bioproduction (e.g., leading to premature shutdown of bioprocesses); this can be avoided through rigorous PCR-based testing of raw materials [74], the use of antibiotics [75], or by engineering strains which efficiently assimilate xenobiotic media compounds and thus outcompete contaminants [76]. It is possible to use specific testing regimes or bacteriophage-resistant mutant strains to avoid phage contamination in microbial bioreactors containing Lactobacillus ssp.…”

Section: Stochastic Perturbations In Bioprocessesmentioning

confidence: 99%

Robustness: linking strain design to viable bioprocesses

Olsson

Rugbjerg

Pianale

et al. 2022

Trends in Biotechnology

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Section: Stochastic Perturbations In Bioprocessesmentioning

confidence: 99%

Robustness: linking strain design to viable bioprocesses

Olsson

Rugbjerg

Pianale

et al. 2022

Trends in Biotechnology

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“…To minimize contamination at the facilities, hop acids, chlorine gas and antibiotics are often used prophylactically to treat active contamination [ 9 – 11 ]. Antibiotic residues in distillery products, such as distillers dried grains have been reported, raising concerns on potential antibiotic resistance and contamination in animal feed [ 12 , 13 ]…”

Section: Introductionmentioning

confidence: 99%

Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Patel,

Lu,

Liu

et al. 2023

Biotechnol Biofuels

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Background Traditional bioethanol fermentation industries are not operated under strict sterile conditions and are prone to microbial contamination. Lactic acid bacteria (LAB) are often pervasive in fermentation tanks, competing for nutrients and producing inhibitory acids that have a negative impact on ethanol-producing yeast, resulting in decreased yields and stuck fermentations. Antibiotics are frequently used to combat contamination, but antibiotic stewardship has resulted in a shift to alternative antimicrobials. Results We demonstrate that endolysin LysMP, a bacteriophage-encoded peptidoglycan hydrolase, is an effective method for controlling growth of LAB. The LysMP gene was synthesized based on the prophage sequence in the genome of Limosilactobacillus fermentum KGL7. Analysis of the recombinant enzyme expressed in E. coli and purified by immobilized metal chelate affinity chromatography (IMAC) showed an optimal lysis activity against various LAB species at pH 6, with stability from pH 4 to 8 and from 20 to 40 °C up to 48 h. Moreover, it retains more than 80% of its activity at 10% ethanol (v/v) for up to 48 h. When LysMP was added at 250 µg/mL to yeast corn mash fermentations containing L. fermentum, it reduced bacterial load by at least 4-log fold compared to the untreated controls and prevented stuck fermentation. In comparison, untreated controls with contamination increased from an initial bacterial load of 1.50 × 107 CFU/mL to 2.25 × 109 CFU/mL and 1.89 × 109 CFU/mL after 24 h and 48 h, respectively. Glucose in the treated samples was fully utilized, while untreated controls with contamination had more than 4% (w/v) remaining at 48 h. Furthermore, there was at least a fivefold reduction in lactic acid (0.085 M untreated contamination controls compared to 0.016 M treated), and a fourfold reduction in acetic acid (0.027 M untreated contamination controls vs. 0.007 M treated), when LysMP was used to treat contaminated corn mash fermentations. Most importantly, final ethanol yields increased from 6.3% (w/v) in untreated contamination samples to 9.3% (w/v) in treated contamination samples, an approximate 50% increase to levels comparable to uncontaminated controls 9.3% (w/v). Conclusion LysMP could be a good alternative to replace antibiotics for mitigation of LAB contamination in biofuel refineries.

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“…According to established evidence, DDGS are more prone to contamination with multiple mycotoxins than the originating grains 5 . Moreover, they can carry residues of drugs with phenols as these are used in the biofermentation process 6 . DDGS also contain phenolic acids 7 …”

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confidence: 99%

Phenol compound toxicity to explain feline pancytopenia?

Brambilla

2021

Veterinary Record

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LETTERS & NOTICESwhen dispensing any progesteronereleasing device to ensure dogs cannot access the product. Second, that colleagues who work with companion animals consider these devices as potential foreign bodies during the sheep breeding season if access seems possible. Finally, that colleagues who encounter cases of ingested devices report these as adverse events to the appropriate manufacturer or the Veterinary Medicines Directorate.

show abstract

Survey of antibiotics residues in DDGS from 14 different states by LCMS

Cited by 4 publications

References 25 publications

Robustness: linking strain design to viable bioprocesses

Robustness: linking strain design to viable bioprocesses

Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Phenol compound toxicity to explain feline pancytopenia?

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