Surgical and postoperative events during permanent fistulation of sheep rumen by the Schalk and Amadon method

Durmic, Zoey; P, McGrath; Wilmot, M. G.; Adams, N. R.; Tan, Tabita; Callahan, Lauren; Mayberry, Chris

doi:10.1111/avj.12343

Cited by 5 publications

(9 citation statements)

References 12 publications

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“…The surgical details of the procedure were based on the method reported by R. J. Komarek and Santra on sheep fistula ( 2 , 20 ), the method of One-step installation was adopted by using T-shaped fistula ( 21 ). In addition, the post-operative care procedure referred to sheep fistula surgery concepts and techniques from Durmic's ( 22 ) study, of this second stage are described elsewhere. Some simple procedures are shown as follows.…”

Section: Methodsmentioning

confidence: 99%

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

Cao

Zhu

et al. 2021

Front. Vet. Sci.

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This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 ± 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry mater intake and the output of dry mater faces 1, 2, 4, 6, 8, 10, 12, 14 days after fistulated surgery. The collection of rumen fluid was arranged at 1d during rumen surgery (DRS1), 3d after rumen surgery (ARS3), and 14d after rumen surgery (ARS14) for volatile fatty acid (VFA) and DNA extraction for sequencing and bioinformatics analysis. There was no difference in DMI, the pH apparent digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber both before and 14 days after surgery. Increases were observed in the acetate and total VFA at ARS3. There was no difference in digestion of dry material, organic material, neutral detergent fiber, and acid detergent fiber before and after surgery. The relative abundance of Bacteroides decreased from 61.96% at DRS1 to 28.85% at ARS3. In comparison with the DRS1 and ARS3, the relative abundance of Firmicutes in the ARS14 increased to 44.58% (P < 0.01). Proteobacteria increased from 11.33% at DRS1 to 51.66% at ARS3 and then decreased to 11.39% at ARS14. Prevotella decreased form 61.06% at DRS1 to 28.04% in the ARS3. Succinivibrio increased from 8.32% at DRS1 to 48.58% at ARS3, but decreased to 10.43% in the ARS14. Compared with DRS1 and ARS3, the ARS14 was higher in the Simpson and Shannon index. As for the BugBase function prediction, rumen fistula surgery increased the microorganism abundance of aerobic and facultative anaerobic phenotype, and anaerobic phenotype was decreased in the ARS3. There was higher microorganism abundance of aerobic phenotype in the ARS14 than before fistula installation. In conclusion, the rumen fistula surgery destroys the anaerobic environment of rumen, leading to differences in rumen microbial diversity and function, but the apparent digestibility and total VFA were not affected.

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Section: Methodsmentioning

confidence: 99%

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

Cao

Zhu

et al. 2021

Front. Vet. Sci.

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“…The surgical operation of rumen fistulas adopts one-step installation method by using T-shaped fistula. The post-operative care procedure referred to sheep fistula surgery concepts and techniques from Durmic et al ( 16 ). The details of the rumen operation and sheep care were described in recently publication by our research team ( 17 ).…”

Section: Methodsmentioning

confidence: 99%

Supplementation of Aspergillus oryzae Culture Improved the Feed Dry Matter Digestibility and the Energy Supply of Total Volatile Fatty Acid Concentrations in the Rumen of Hu Sheep

Guo

Zhang

et al. 2022

Front. Nutr.

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The objective of the present study was to investigate the effects of feeding different amounts of Aspergillus oryzae culture (AOC) on the degradation rate of various feeds for 24 h, rumen fermentation parameters, microbial community, and blood cell composition of Hu sheep. Sixteen castrated and fattening adult Hu sheep with permanent rumen fistula were randomly divided into four groups (four sheep per group) based on body weight (64.62 ± 5.83 kg). The experiment was repeated for two periods to ensure eight replicates for each treatment, and each period consisted of 28 days, including a 7-d of transition, a 14-d of pre-feeding, and a 7-d of sample collection. The control group (CON) received a basal diet without AOC, and the other groups were fed basal diet supplemented with 10 g/d, 20 g/d, and 40 g/d AOC, respectively, every day before the morning feeding. Supplementation with 20 g/d and 40 g/d AOC significantly increased (P < 0.05) the total volatile fatty acids (TVFAs) content, the molar ratio of butyric acid, and the 24 h dry matter (DM) degradation rate of alfalfa hay and corn straw. When fed 40 g/d AOC, the DM degradation rate of corn germ meal and the relative abundance of Kiritimatiellaeota were significantly increased (P < 0.05), but the ratio of acetic acid to propionic acid (A/P) was significantly reduced (P = 0.04). In conclusion, supplementation with AOC for Hu sheep could improve feed DM digestibility and increase the energy supply of TVFAs concentration in the rumen. Based on the feed conditions of the present study, supplementation 40 g/d of AOC could increase the production efficiency of sheep while higher level have to further investigate.

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“…The second production animal paper reports on an experimental technique of placing a permanent fistula into the rumen . As the rumen is a vat of a complex mix of plant material, fungi, protozoa and bacteria, it cannot be replicated in vitro, and different methods have been suggested for making fistulae into the4 flank to allow access to ruminal contents.…”

Section: Production Animalsmentioning

confidence: 99%

In this issue – July 2015

2015

Aust Veterinary J

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Surgical and postoperative events during permanent fistulation of sheep rumen by the Schalk and Amadon method

Abstract: Rumen fistulation of sheep by the Schalk and Amadon method, as described here, is a comparatively simple, safe and ethical procedure, with minimal effect on or complications for the animal.

Cited by 5 publications

References 12 publications

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

Supplementation of Aspergillus oryzae Culture Improved the Feed Dry Matter Digestibility and the Energy Supply of Total Volatile Fatty Acid Concentrations in the Rumen of Hu Sheep

In this issue – July 2015

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