Surflan™ and oryzalin impair reproduction in the teleost medaka (Oryzias latipes)

Abstract

We conducted studies to determine if the xenoestrogens Surflan and its active ingredient oryzalin, affect indices of reproductive fitness in medaka (Oryzias latipes). Oryzalin (0.5, 0.25mg/l) or Surflan (2.0 microl/l) and oryzalin (0.5mg/l) significantly increased the mean number of non-fertilized eggs produced by treated females paired with untreated males, or by untreated females paired with treated males. Oryzalin (1.0, 0.5, 0.25mg/l) and Surflan (3.8, 2.0, 1.0 microl/l) significantly affected the time to hatch of eggs from treated females paired with untreated males, and from untreated females paired with treated males. Surflan (3.8, 2.0, 1.0 microl/l) induced intersex lesions in 80-100% of males. Oryzalin-exposed males exhibited a significant increase in the incidence of necrotic spermatids and necrotic spermatogonia, while oryzalin-exposed females had significantly fewer immature oocytes and an increase in the occurrence of hyperplastic ovaries. Our results indicate that Surflan and oryzalin affect both reproduction and gonadal histology in male and female medaka.

Histopathological changes in the reproductive system (ovaries and testes) of Oreochromis mossambicus following exposure to DDT

“…Atretic oocytes were characterised by loss of oocyte shape, a distorted chorion and a disorganized and fragmented yolk (Hall et al, 2007) (Fig. 9).…”

Effects of Herbicides on Fish

“…Full life-cycle tests are resource-intensive, and the alternative shortterm reproduction assay using fathead minnows (P. promelas) (Ankley et al, 2001) has only recently been developed and validated. The effects of oryzalin (a dinitroaniline herbicide, Table 7.8) on reproduction and development of medaka (O. latipes) were reported by Hall et al (2007). Treatment of either males and females, or both, resulted in an increase in the number of nonfertilized eggs produced.…”

Unraveling the Pollution and Discharge of Aminophenyl Sulfone Compounds, Sulfonamide Antibiotics, and Their Acetylation Products in Municipal Wastewater Treatment Plants

“…In comparison with sulfonamides, other ASCs (e.g., oryzalin) have similarly detrimental effects on nontarget organisms or even cause other harmful effects, such as endocrine-disrupting effects 15 and reproduction toxicity. 16 Similarly, some ASCs (e.g., sotalol) are not readily biodegradeable 17 (which do not contain the core structure) are used in a wide range of fields. 17−19 However, most studies have focused on a few well-known sulfonamides, with minimal consideration for other ASCs.…”

“…Numerous ASCs that contain a core structure (e.g., C1­(CCC­(C C1)­N­([H])­[H])­[S]­(O)O) have been used in the pharmaceutical (e.g., antiarrhythmic drugs, antihypertensives, antipsychotics, and sulfonamide antibiotics), − agricultural (e.g., herbicides), textile (e.g., colorants), and chemical industries (e.g., indicators and intermediates). , Generally, the toxicity of chemicals was dependent on not only the core structure but also the functional group substitution. In comparison with sulfonamides, other ASCs (e.g., oryzalin) have similarly detrimental effects on nontarget organisms or even cause other harmful effects, such as endocrine-disrupting effects and reproduction toxicity . Similarly, some ASCs (e.g., sotalol) are not readily biodegradeable and persist in the environment.…”