Surfactant-Enhanced Desorption/Ionization on Silicon Mass Spectrometry

Nordström, Anders; Apon, Junefredo V.; Uritboonthai, Wilasinee; Go, Eden P.; Siuzdak, Gary

doi:10.1021/ac051398q

Cited by 54 publications

(38 citation statements)

References 54 publications

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“…The effect was most pronounced for carbamazepine, which is a polycyclic alkaline drug. The detection limit achieved for propranolol desorbed from the surface of oxidized GCB is comparable to that for DIOS chip [27].…”

Section: Enhancement Of Saldi-ms Signal-to-noise Ratiosmentioning

confidence: 84%

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

Amini

Shariatgorji

Thorsén

2009

J. Am. Soc. Mass Spectrom.

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The signal intensity of low-molecular-weight compounds analyzed using surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS) was significantly enhanced when oxidized graphitized carbon black (GCB) particles were used as the desorption/ionization surface. The surface of oxidized GCB contains more carboxylic acid groups than non-oxidized GCB. Carboxylic acid groups enhance the efficiency of the ionization process and the desorption of more hydrophobic compounds. A common pharmaceutical compound, propranolol, was successfully extracted from Baltic Sea blue mussels and quantified using oxidized GCB as the SALDI surface, whereas deuterated propranolol was used as the internal standard. The calibration curve showed a wide linear dynamic range of response (0.1-20 g/mL) and good reproducibility (RSD Ͻ 10%). It was not possible to detect propranolol in Baltic Sea blue mussels when non-oxidized GCB was used as the SALDI surface. (J Am Soc Mass

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Section: Enhancement Of Saldi-ms Signal-to-noise Ratiosmentioning

confidence: 84%

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

Amini

Shariatgorji

Thorsén

2009

J. Am. Soc. Mass Spectrom.

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“…It was found that the addition of ammonium citrate to the biological materials helps to reduce contaminant interferences present in biological solutions. Another study used perflorinated surfactants to clean up tryptic digests prior to DIOS analysis and identification of the peptide fragments (Nordstrom et al, 2006). The use of DIOS to determine enzymatic activity was also further expanded to obtain high throughput screening information (Shen et al, 2004a), including DIOS plates with 96 and 384 spots to accomplish high throughput screening of enzyme activity and inhibition.…”

Section: Protein Analysismentioning

confidence: 99%

Matrix‐free methods for laser desorption/ionization mass spectrometry

Peterson

2006

Mass Spectrometry Reviews

284

249

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Matrix assisted laser desorption/ionization (MALDI) is a soft ionization mass spectrometric method that has become a preeminent technique in the analysis of a wide variety of compounds including polymers and proteins. The main drawback of MALDI is that it is difficult to analyze low molecular weight compounds (<1,000 m/z) because the matrix that allows MALDI to work interferes in this mass range. In recent years there has been considerable interest in developing laser desorption/ionization (LDI) techniques for the analysis of small molecules. This review examines the approaches to matrix-free LDI mass spectrometry including desorption/ionization on silicon (DIOS), sol-gels, and carbon-based microstructures. For the purposes of this review matrix-free methods are defined as those that do not require matrix to be mixed with the analyte and therefore does not require co-crystallization. The review will also examine mechanisms of ionization and applications of matrix-free LDI-MS.

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“…Although DIOS [17] and silicon-NP-assisted LDI [13] also have higher salt tolerance, the preparation of AuNPs is facile and does not require the use of UV light, electrochemical etching, HF etching, or fluorinated derivatives. Additionally, the detection of carbohydrates by DIOS is successful only in the presence of perfluorinated surfactants, which were spotted on the DIOS chip [30].…”

Section: Carbohydrates Gm1 Indolamines and Angiotensin Imentioning

confidence: 99%

Gold nanoparticles as assisted matrices for the detection of biomolecules in a high-salt solution through laser desorption/ionization mass spectrometry

Lin

et al. 2009

J. Am. Soc. Mass Spectrom.

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Citrate-capped gold nanoparticles (AuNPs) serve as matrices for the determination of biomolecules in a high-salt solution through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In the case of using 2,5-dihydroxybenzoic acid (2,5-DHB) as a matrix, the signal intensities of neutral steroids were severely suppressed in a high-salt solution. A high concentration of NaCl caused the formation of the sodium adduct ions during the desorption/ionization process, resulting in a decrease of the signal intensities of the protonated ions. In comparison, by applying AuNP-assisted LDI-TOF-MS, the signal intensities of neutral steroids remained almost constant when the concentration of NaCl was increased to 500 mM. Because the use of citrate-capped AuNPs as matrices primarily offers alkali metal ion adducts, AuNP matrices have a higher tolerance to high NaCl concentrations relative to that of 2,5-DHB matrices. The relevant phenomena are also discovered in the case of analysis of neutral carbohydrate, monosialoganglioside, indolamine, and angiotensin I. The quantification of small molecules in a high-salt solution has been accomplished by AuNP-assisted LDI-TOF-MS coupled to a unique sample preparation, in which samples are deposited onto the sample plate before AuNPs. The present method has been further applied to the determination of urea, creatinine, uric acid, and glucose in a urine sample. (J Am Soc Mass Spectrom 2009, 20, 875-882)

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Surfactant-Enhanced Desorption/Ionization on Silicon Mass Spectrometry

Cited by 54 publications

References 54 publications

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

Matrix‐free methods for laser desorption/ionization mass spectrometry

Gold nanoparticles as assisted matrices for the detection of biomolecules in a high-salt solution through laser desorption/ionization mass spectrometry

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