Surface wettability and platelet adhesion studies on Langmuir–Blodgett films

Lee, Yuh Lang; Chen, Chi‐Yun

doi:10.1016/s0169-4332(02)01235-7

Cited by 28 publications

(16 citation statements)

References 40 publications

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“…[46]. The studies reported in the literature, involving DMPC, CHOL and DMPC:CHOL (7:3) mixture show slight differences that can be assigned to different experimental conditions such as buffer solution and temperature [47][48][49][50] The results obtained in this work remain within the dispersion found for the reported studies.…”

Section: Dsc Analysissupporting

confidence: 83%

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Serro

Galante

Kozica

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Different types of lipid bilayers/monolayers have been used to simulate the cellular membranes in the investigation of the interactions between drugs and cells. However, to our knowledge, very few studies focused on the influence of the chosen membrane model upon the obtained results. The main objective of this work is to understand how do the nature and immobilization state of the biomembrane models influence the effect of the local anaesthetic tetracaine (TTC) upon the lipid membranes. The interaction of TTC with different biomembrane models of dimyristoylphosphatidylcholine (DMPC) with and without cholesterol (CHOL) was investigated through several techniques. A quartz crystal microbalance with dissipation (QCM-D) was used to study the effect on immobilized liposomes, while phosphorus nuclear magnetic resonance ( 31 P-NMR) and differential scanning calorimetry (DSC) were applied to liposomes in suspension. The effect of TTC on Langmuir monolayers of lipids was also investigated through surface pressure-area measurements at the air-water interface. The general conclusion was that TTC has a fluidizing effect on the lipid membranes and, above certain concentrations, induced membrane swelling or even solubilization. However, different models led to variable responses to the TTC action. The intensity of the disordering effect caused by TTC increased in the following order: supported liposomes < liposomes in solution < Langmuir monolayers. This means that extrapolation of the results obtain in in vitro studies of the lipid/anaesthetic interactions to in vivo conditions should be done carefully.

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Section: Dsc Analysissupporting

confidence: 83%

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Serro

Galante

Kozica

et al. 2014

Colloids and Surfaces B: Biointerfaces

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“…27 No matter what kind of acidic ethanolic washing solutions were utilized, the positively charged pure À ÀNH 2 terminated-SAM exhibited a less amount of adherent platelets than the anionic pure À ÀCOOH terminated counterpart. Lee and Chen 11 have demonstrated similar results in LB monolayer, in which fewer platelets adhesion on a À ÀNH 2 terminated LB film was noted as compared with the À ÀCOOH terminated one. It is surprising to notice that the corresponding surface mole fraction of the À ÀNH 2 terminal functionality (X NH 2 ,surface) on the least platelet adherent mixed SAMs in both acidic ethanolic treatment series were all close to 50%.…”

Section: Contact Angle Measurementmentioning

confidence: 58%

“…Similar findings were also reported on the monolayer formed by the LB film of zwitterionic lipids of DPPC and DMPC. 11 Surface preabsorbed protein has been recognized as an important factor in mediating the subsequent interactions between platelets and biomaterials. 27 Lestelius et al has found that the SAM formed by the alkanethiol with charge-neutral À ÀOH terminal group showed a low deposition of plasma protein in general.…”

Section: Contact Angle Measurementmentioning

confidence: 99%

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Surface characterization and platelet adhesion studies for the mixed self‐assembled monolayers with amine and carboxylic acid terminated functionalities

Chuang

Lin

2007

J Biomedical Materials Res

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The mixed self-assembled monolayers (SAM) prepared from long chain alkanethiols, HS(CH(2))(11)NH(2) and HS(CH(2))(10)COOH, on gold are employed as the model surface for investigating the interactions between the biological environment and synthetic surface. A distinctive SAM preparation scheme was utilized in this investigation. The triethylamine was added to the alkanethiol solution during SAM formation and then followed by additional rinsing of SAM with 10% CH(3)COOH or 1% HCl ethanolic solution. The contact angle values of NH(2) + COOH mixed SAMs were between those of the pure SAMs, except that it was prepared with solution mole fraction of amine-terminated alkanethiol at 0.2. X-ray photoelectron spectroscopy (XPS) analysis has indicated that these two distinctive SAM preparation procedures had both resulted in a reduction in oxidized sulfur species on pure --NH(2) terminated SAM. However, the procedure utilizing 1% HCl ethanolic washing solution was more effective in reducing the unbound thiol fraction and to form a pure --NH(2) SAM with better quality. XPS analysis has also revealed that the surface of NH(2) + COOH mixed SAMs was "amine-rich". In vitro platelet adhesion assay has shown that the amount of adherent platelets on pure positive charged --NH(2) terminated SAM is less than that on anionic --COOH terminated counterpart in both acidic ethanolic washing schemes. Moreover, the lowest platelet adhesion density was noted on the mixed SAM surfaces with surface amine mole fraction at 0.51 and 0.57. This finding suggests that the surface charge with near neutrality might be of importance in reducing platelet adhesion and activation on artificial biomaterial.

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“…In fact, Lee and Chen [11] have shown that the blood platelet adhesion on substrates is largely affected by the pretreatment with, among others, DPPC (dipalmitoylphosphatidylcholine, or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and DMPC (dimyristoylphosphatidylcholine or 1,2-dimyristoyl-sn-glycero-3-phosphocholine).…”

Section: Introductionmentioning

confidence: 99%

Surface modification of glass plates and silica particles by phospholipid adsorption

Chibowski

Delgado

Rudzka

et al. 2011

Journal of Colloid and Interface Science

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Surface wettability and platelet adhesion studies on Langmuir–Blodgett films

Cited by 28 publications

References 40 publications

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Surface characterization and platelet adhesion studies for the mixed self‐assembled monolayers with amine and carboxylic acid terminated functionalities

Surface modification of glass plates and silica particles by phospholipid adsorption

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