Surface plasmon resonance sensors: review

Homola, Jiřı́; Yee, Sinclair S.; Gauglitz, Günter

doi:10.1016/s0925-4005(98)00321-9

Cited by 5,046 publications

(3,101 citation statements)

References 117 publications

Supporting

Mentioning

3,037

Contrasting

Unclassified

Order By: Relevance

“…Besides, the other colored parts mainly refer to the cytoplasm, corresponding to the smaller shift of resonant frequencies stemming from the lower refractive index of the cytoplasm. [ 28 ] In short, the morphologic observation by the label-free SRRM exhibits the similar result to the confocal fl uorescent optical microscopy image (as shown in Figure 3 b). The purple part is the nuclei of hMSCs labeled by 4'-6-diamidino-2-phenylindole (DAPI).…”

Section: Methodssupporting

confidence: 71%

Label‐Free, Coupler‐Free, Scalable and Intracellular Bio‐imaging by Multimode Plasmonic Resonances in Split‐Ring Resonators

et al. 2012

View full text Add to dashboard Cite

Section: Methodssupporting

confidence: 71%

Label‐Free, Coupler‐Free, Scalable and Intracellular Bio‐imaging by Multimode Plasmonic Resonances in Split‐Ring Resonators

et al. 2012

View full text Add to dashboard Cite

“…15,16 The home-built SPR liquid biosensing system used in this study has been described and characterized in more detail elsewhere. 45,46 A dual-channel Teflon flow cell with two independent, low volume parallel flow channels was used. A peristaltic pump (Ismatec) was utilized to deliver solution to the two chambers of the flow cell at a rate of 50 L/min.…”

Section: Spr Measurementsmentioning

confidence: 99%

XPS, TOF-SIMS, NEXAFS, and SPR Characterization of Nitrilotriacetic Acid-Terminated Self-Assembled Monolayers for Controllable Immobilization of Proteins

2008

View full text Add to dashboard Cite

For immobilization of proteins onto surfaces in a specific and controlled manner it is important to start with a well-defined surface that contains specific binding sites surrounded by a nonfouling background. For immobilizing histidine-tagged (his-tagged) proteins, surfaces containing nitrilotriacetic acid (NTA) headgroups and oligo(ethylene glycol) (OEG) moieties are a widely used model system. The surface composition, structure and reactivity of mixed NTA/OEG selfassembled monolayers (SAMs) on Au substrates were characterized in detail using x-ray photoelectron spectroscopy (XPS), near-edge x-ray absorption fine structure spectroscopy (NEXAFS), time-of-flight secondary ion mass spectrometry (ToF-SIMS) and surface plasmon resonance (SPR) biosensoring. XPS results for sequential adsorption of NTA thiols followed by OEG thiols showed that OEG molecules were incorporated into an incompletely formed NTA monolayer until a complete mixed SAM was formed. The surface concentration of NTA headgroups was estimated to be 0.9-1.3 molecule/nm 2 in the mixed NTA/OEG monolayers, compared to 1.9 molecule/nm 2 in pure NTA monolayers. Angle-dependent XPS indicated NTA headgroups were slightly reoriented toward an upright position after OEG incorporation and polarization-dependent NEXAFS results indicated increased ordering of the alkane chains of the molecules. Nitrogen-containing and OEG-related secondary ion fragments from the ToF-SIMS experiments confirmed the presence of NTA headgroups and OEG moieties in the monolayers. A multivariate peak intensity ratio was developed for estimating the relative NTA concentration in the outermost (10 Ǻ) of the monolayers. SPR measurements of a his-tagged, humanized antilysozyme variable fragment (HuLys Fv) immobilized onto Ni(II)-treated mixed NTA/OEG and pure NTA monolayers demonstrated the reversible, site-specific immobilization of his-tagged HuLys Fv (108 -205 ng/cm 2 ) with dissociation rates (k off ) between 1.0x10 −4 and 2.1x10 −5 s −1 , both depending on the NTA surface concentration and orientation. The monolayers without Ni(II) treatment exhibited low nonspecific adsorption of his-tagged HuLys Fv ( < 2ng/cm 2 ).

show abstract

“…Besides, optical sensors eliminate the need for uorescent or radioactive labeling of molecules, which are relatively time-consuming, expensive and large area demanding [1]. Various label-free optical sensing techniques are proposed which are based on surface plasmon resonance [2], interferometry [3 5], waveguides [6,7], optical bers [8] and ring resonators [9 11]. Although, some of these techniques are now mature and even commercialized, these techniques are not very practical for on-chip integration with incorporation of light sources and detectors.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Refractive Index Sensitivity in Photonic Crystal Waveguide-Based Sensors by Selective Infiltration

Bagci

Akaoglu

2013

Acta Phys. Pol. A

View full text Add to dashboard Cite

We present a liquid refractive index sensor based on a photonic crystal waveguide slab structure. Sensing mechanism employed in this study is based on the shift in cut-o wavelength as the lattice holes are selectively in ltrated. Three-dimensional plane-wave expansion and nite-di erence time-domain methods are used to determine the band structure and transmission spectra, respectively. First, the sensitivity of the device is analyzed for the structure where only the rst rows of holes adjacent to the line-defect are in ltrated. In addition, this analysis is repeated for a range of hole diameters. Second, the e ects of in ltrated holes which are placed in the line-defect are investigated. As these in ltrated central holes are introduced, the proposed device exhibits 5.3 times improved sensitivity.

show abstract

Surface plasmon resonance sensors: review

Cited by 5,046 publications

References 117 publications

Label‐Free, Coupler‐Free, Scalable and Intracellular Bio‐imaging by Multimode Plasmonic Resonances in Split‐Ring Resonators

Label‐Free, Coupler‐Free, Scalable and Intracellular Bio‐imaging by Multimode Plasmonic Resonances in Split‐Ring Resonators

XPS, TOF-SIMS, NEXAFS, and SPR Characterization of Nitrilotriacetic Acid-Terminated Self-Assembled Monolayers for Controllable Immobilization of Proteins

Enhancement of Refractive Index Sensitivity in Photonic Crystal Waveguide-Based Sensors by Selective Infiltration

Contact Info

Product

Resources

About