Surface morphology of contact lenses probed with microscopy techniques

Guryča, Vilém; Hobzová, Radka; Přádný, Martin; Širc, Jakub; Michálek, Jiřı́

doi:10.1016/j.clae.2007.02.010

Cited by 59 publications

(67 citation statements)

References 24 publications

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“…The spin casting method generates contact lenses with the smoothest surfaces, followed by cast-molding and then lathe-cut lenses (Guryca et al, 2007;Grobe, 1996). All the lenses presented here were cast-molded, and their roughness parameters were similar to the ranges reported for other non surface-treated cast-molded lenses (Guryca et al, 2007).…”

Section: Contactsupporting

confidence: 51%

“…In effect, AFM has proved useful for characterizing tear deposits on worn soft contact lens surfaces (Baguet et al, 1995;Rebeix et al, 2000) or characterizing the rigid gas permeable contact lens surface (Bruinsma et al, 2003). In fact, detailed information about the surface quality of CL has been studied previously by Atomic Force Microscopy (AFM) (Bhatia et al, 1997;Baguet et al, 1993;Baguet et al, 1995;Bruinsma et al, 2003;Gonzalez-Meijome et al, 2006a;Gonzalez-Meijome et al, 2009;Giraldez et al, 2010c) and Cryo-SEM (Gonzalez-Meijome et al, 2006b;Guryca et al, 2007). AFM is a very powerful tool for high resolution examination of hydrated CL surface structure.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

“…Thus, the roughness differences between lenses cannot be attributed only to the manufacturing procedure. Besides the mode of elaboration, other authors have linked the presence of methacrylic acid (MA) (Baguet et al, 1993) or a reduced water content (Guryca et al, 2007;Vermeltfoort et al, 2004) to a greater lens surface roughness.…”

Section: Contactmentioning

confidence: 99%

“…The parameters generally used to quantify roughness include height parameters such as average roughness (Ra), mean-square-roughness (Rms) and Maximum Roughness (Rmax) (Baguet et al, 1993;Guryca et al, 2007;Bhatia et al, 1997;Hinojosa Rivera and Reyes Melo, 2001;Lira et al, 2008;Gonzalez-Meijome et al, 2009;Giraldez et al, 2010a;Giraldez et al, 2010c;Gonzalez-Meijome et al, 2006a). Ra is the average deviation or arithmetic mean of the profile from the mean line; it is universally accepted and is the most used international parameter of roughness.…”

Section: Height Parametersmentioning

confidence: 99%

“…Atomic force microscopy (AFM) provides detailed information on the surface characteristics of contact lenses (Bhatia et al, 1997;Baguet et al, 1993;Baguet et al, 1995;Bruinsma et al, 2003;Lira et al, 2008;Guryca et al, 2007;Gonzalez-Meijome et al, 2006a;Gonzalez-Meijome et al, 2009;Teichroeb et al, 2008;Maldonado-Codina and Efron, 2005) and is a powerful tool for the high resolution examination of the structure of the hydrated contact lens surface. The method has the advantages that it avoids artefacts due to dehydration and coating (Bhatia et al, 1997;Kim et al, 2002), and allows for non-destructive surface topography and roughness measurements.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

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Hydrogel Contact Lenses Surface Roughness and Bacterial Adhesion

Giráldez¹,

Yebra‐Pimentel²

2012

Ocular Diseases

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Section: Contactsupporting

confidence: 51%

Section: Atomic Force Microscopymentioning

confidence: 99%

Section: Contactmentioning

confidence: 99%

Section: Height Parametersmentioning

confidence: 99%

Section: Atomic Force Microscopymentioning

confidence: 99%

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Hydrogel Contact Lenses Surface Roughness and Bacterial Adhesion

Giráldez¹,

Yebra‐Pimentel²

2012

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ACR Announcements

2012

Arthritis & Rheumatism

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A new process has been developed to determine the biological activity of an intact layer of lysozyme deposited onto a biomaterial surface. This process is applied to a number of common hydrogel contact lenses. The activity of the surface‐adsorbed protein is measured using a standard micrococcal activity assay, with extra steps to distinguish between protein on the surface and protein in solution. This is in contrast to protein extraction work in which the activity of all adsorbed protein is measured. For ionic materials, which are known to deposit large amounts of protein, particularly positively charged proteins such as lysozyme, there is evidence for loosely bound protein re‐entering the solution, thus making it impossible to truly separate out the surface‐adsorbed protein. This optimized process provides the first quantification of the biological activity of an intact layer of surface‐adsorbed protein at a hydrogel interface. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 755–764, 2013.

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Measuring the kinetics and activity of adsorbed proteins: In vitro lysozyme deposited onto hydrogel contact lenses over short time periods

Hall

Jones

Forrest

2012

J Biomedical Materials Res

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A new process has been developed to determine the biological activity of an intact layer of lysozyme deposited onto a biomaterial surface. This process is applied to a number of common hydrogel contact lenses. The activity of the surface-adsorbed protein is measured using a standard micrococcal activity assay, with extra steps to distinguish between protein on the surface and protein in solution. This is in contrast to protein extraction work in which the activity of all adsorbed protein is measured. For ionic materials, which are known to deposit large amounts of protein, particularly positively charged proteins such as lysozyme, there is evidence for loosely bound protein re-entering the solution, thus making it impossible to truly separate out the surface-adsorbed protein. This optimized process provides the first quantification of the biological activity of an intact layer of surface-adsorbed protein at a hydrogel interface.

show abstract

Surface morphology of contact lenses probed with microscopy techniques

Cited by 59 publications

References 24 publications

Hydrogel Contact Lenses Surface Roughness and Bacterial Adhesion

Hydrogel Contact Lenses Surface Roughness and Bacterial Adhesion

ACR Announcements

Measuring the kinetics and activity of adsorbed proteins: In vitro lysozyme deposited onto hydrogel contact lenses over short time periods

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