Surface modification of polydimethylsiloxane (PDMS) induced proliferation and neural-like cells differentiation of umbilical cord blood-derived mesenchymal stem cells

Abstract

Stem cell-based therapy has recently emerged for use in novel therapeutics for incurable diseases. For successful recovery from neurologic diseases, the most pivotal factor is differentiation and directed neuronal cell growth. In this study, we fabricated three different widths of a micro-pattern on polydimethylsiloxane (PDMS; 1, 2, and 4 microm). Surface modification of the PDMS was investigated for its capacity to manage proliferation and differentiation of neural-like cells from umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs). Among the micro-patterned PDMS fabrications, the 1 microm-patterned PDMS significantly increased cell proliferation and most of the cells differentiated into neuronal cells. In addition, the 1 microm-patterned PDMS induced an increase in cytosolic calcium, while the differentiated cells on the flat and 4 microm-patterned PDMS had no response. PDMS with a 1 microm pattern was also aligned to direct orientation within 10 degrees angles. Taken together, micro-patterned PDMS supported UCB-MSC proliferation and induced neural like-cell differentiation. Our data suggest that micro-patterned PDMS might be a guiding method for stem cell therapy that would improve its therapeutic action in neurological diseases.

Future of cord blood for non-oncology uses

“…In vitro by RT-PCR, alizarin red and von Kossa-staining, ALP detection, immunohistochemistry and western blot 8,31,[110][111][112][113] In vitro by safranin-O staining, RT-PCR and immunohistochemistry 8,31 In vitro by immunohistochemistry, RT-PCR, calcium imaging and western blot [114][115][116][117] In vivo in parkinsonian rat model, ischemic rat model [118][119][120][121][122][123] In vitro by immunohistochemistry, RT-PCR, PAS-staining, urea and albumin production, western blot and LDL uptake 31,94,124,125 CD34/AC133-positive (hematopoietic progenitor) cells In vitro by RT-PCR 126 In vivo in NOD/SCID mouse model 95,96 Not defined cells from MNC In vitro by immunohistochemistry, whole-cell patch-clamp, western blot and RT-PCR [127][128][129][130][131] In vivo in spinal cord injury and intra-brain injection in rat and NOD/SCID mouse model 73,76,[80][81][82][83][84][86][87][88][89][90][91][92]<...>…”

The culture and differentiation of amniotic stem cells using a microfluidic system

“…A well-confined culture area constructed by the PDMS was used to culture and differentiate AFMSCs sequentially. By using the PDMS material, its oxygen and CO 2 permeability may provide a suitable culturing and differentiating environment for stem cells (Kim et al 2008). Typically, it is composed of culture areas, micropumps, microgate structures, seeding reservoirs, waste reservoirs and fluid microchannels for connecting these devices.…”

Micro- and Nanoengineering Approaches to Control Stem Cell-Biomaterial Interactions

“…An excellent example is the alignment of cells along microgrooves, a phenomenon known as contact guidance [28,29,30,31]. Microstructures also influence basic cellular processes such as adhesion [32,33,34,35,36,37], migration [38,39,40], proliferation [41,42] and differentiation [43,44]. Recently, there has been significant interest towards utilizing microscale topographies in controlling stem cell behavior.…”

“…Likewise, mouse mesenchymal stem cells (mMSCs) were shown to exhibit an elongated morphology when cultured on microgrooves [46] (Figure 2B). A number of studies have investigated the effect of microgroove widths on differentiation of MSCs [44,47]. It was shown that the differentiation of MSCs into neural-like cells was less pronounced on the 4 μm wide microgrooves compared to narrower 1 and 2 μm microgrooves.…”