Surface modification of Cu nanoparticles coated commercial titanium in the presence of tryptophan: Comprehensive electrochemical and spectroscopic investigations

In Situ and Ex Situ Raman Studies of Cysteine’s Behavior on a Titanium Surface in Buffer Solution

“…Raman spectra give a fingerprint of a molecule and can be analyzed for molecules in different states [30,31]. Raman scattering can be drastically enhanced for molecules adsorbed or in close proximity to the metallic surface, resulting in a very sensitive surface-enhanced Raman spectroscopy technique [32][33][34][35][36]. Vibration fingerprints depend on many parameters, i.e., that is, the molecular environment (pH of the buffer solution) [37,38] and properties of the metallic surface [39].…”

“…To better understand the adsorption behavior of Cys on the Ti surface, ex situ SERS spectroscopy measurements obtained for dried samples in the same conditions were provided. Despite the many investigations conducted on biomolecules immobilized on different metallic surfaces [33][34][35]41], there is still a lack of studies in this area. Detailed analysis of the adsorption process of Cys on the Ti surface, in the presence of buffer solution, in a different state, under control conditions, can be very important in the preparation of bioactive materials (i.e., coated by organic layers).…”

“…It should be noted that the preparation of samples for measurements has a significant impact on the spectral information obtained. Parameters such as the sample's condition [35], type of solvent [48], buffer solutions [49], and pH [30,33,50] can influence the recorded spectra.…”

“…It should be noted that the preparation of samples for measurements has a significant impact on the spectral information obtained. Parameters such as the sample's condition [35], type of solvent [48], buffer solutions [49], and pH [30,33,50] can influence the recorded spectra. The reference Raman spectra for Cys (10 −1 M/PBS) were obtained in solution at a pH from 1 to 7.4 and do not differ significantly from each other.…”

Quantitative and qualitative analyses of drug adsorption on silver nanoparticle monolayers: QCM, SERS, and TEIRA nanospectroscopy studies

“…Previously published results prove that the surface-enhanced technique combining AFM with IR spectroscopy, namely AFM-SEIRA, enables the detailed characterization of how molecules adsorb on the MeNP monolayers. [25][26][27][28] In these studies, TEIRA nanospectroscopy was used to characterize the molecular structure of erlotinib, a drug approved for non-small cell lung cancer (NSCLC) therapy, immobilized on the monolayers formed from silver nanoparticles prepared using hydroxylamine hydrochloride (HH). It is well known that the suspensions of AgNPs prepared with the use of HH are particularly attractive for spectroscopic techniques.…”