Surface modification of 3D-printed porous scaffolds via mussel-inspired polydopamine and effective immobilization of rhBMP-2 to promote osteogenic differentiation for bone tissue engineering

Lee, Sang Jin; Lee, Dong-Hyun; Yoon, Taek Rim; Kim, Hyung Keun; Jo, Ha Hyeon; Park, Ji Sun; Lee, Jun Hee; Kim, Wan Doo; Kwon, Il Keun; Park, Su A

doi:10.1016/j.actbio.2016.02.006

Cited by 182 publications

(132 citation statements)

References 55 publications

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“…(61) Lee et al, on the other hand, performed post-fabrication conjugation of biomolecules by using mussel-inspired adhesive chemistry to adhere bone morphogenetic protein-2 (BMP-2) to the surface of a 3D printed PCL scaffold. (62) While relatively few examples exist of biomolecule conjugation to 3DP scaffolds, the authors of this review could not find literature in which biomolecule patterns or gradients were generated on 3DP scaffolds by bioconjugation strategies. Future work in bioprinting could thus apply bioconjugation strategies such as the usage of “click” and activated ester chemistry to produce biofunctionalized inks for subsequent gradient and pattern printing.…”

Section: Fabricating Spatiotemporal Growth Factor Patternsmentioning

confidence: 87%

“…Existing reviews have summarized the considerations related to bioconjugation of biomolecules to tissue engineering scaffolds,(59,60) and these strategies have occasionally been applied to 3DP scaffolds. (59,61,62) Gao et al, for instance, conjugated acrylated peptides to acrylated PEG during inkjet printing to generate crosslinked hydrogels with bioactive peptide presentation through the scaffold. (61) Lee et al, on the other hand, performed post-fabrication conjugation of biomolecules by using mussel-inspired adhesive chemistry to adhere bone morphogenetic protein-2 (BMP-2) to the surface of a 3D printed PCL scaffold.…”

Section: Fabricating Spatiotemporal Growth Factor Patternsmentioning

confidence: 99%

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Spatiotemporal control of growth factors in three-dimensional printed scaffolds

Bittner

Guo

2018

Bioprinting

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Three-dimensional printing (3DP) has enabled the fabrication of tissue engineering scaffolds that recapitulate the physical, architectural, and biochemical cues of native tissue matrix more effectively than ever before. One key component of biomimetic scaffold fabrication is the patterning of growth factors, whose spatial distribution and temporal release profile should ideally match that seen in native tissue development. Tissue engineers have made significant progress in improving the degree of spatiotemporal control over which growth factors are presented within 3DP scaffolds. However, significant limitations remain in terms in pattern resolution, the fabrication of true gradients, temporal control of growth factor release, the maintenance of growth factor distributions against diffusion, and more. This review summarizes several key areas for advancement of the field in terms of improving spatiotemporal control over growth factor presentation, and additionally highlights several major tissues of interest that have been targeted by 3DP growth factor patterning strategies.

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Section: Fabricating Spatiotemporal Growth Factor Patternsmentioning

confidence: 87%

Section: Fabricating Spatiotemporal Growth Factor Patternsmentioning

confidence: 99%

Spatiotemporal control of growth factors in three-dimensional printed scaffolds

Bittner

Guo

2018

Bioprinting

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“…PCL scaffolds delivery of rhBMP-2 released in a sustained manner exhibit good cellular activity for both cell proliferation and osteogenic activity40. Major advances in bone tissue engineering with scaffolds have been achieved through the use of growth factors, drugs, and gene delivery systems targeting osteogenic differentiation, though with some drawbacks891011.…”

Section: Discussionmentioning

confidence: 99%

An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)

Chen

Huang

et al. 2017

Sci Rep

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Although great efforts are being made using growth factors and gene therapy, the repair of bone defects remains a major challenge in modern medicine that has resulted in an increased burden on both healthcare and the economy. Emerging tissue engineering techniques that use of combination of biodegradable poly-lactic-co-glycolic acid (PLGA) and mesenchymal stem cells have shed light on improving bone defect healing; however, additional growth factors are also required with these methods. Therefore, the development of novel and cost-effective approaches is of great importance. Our in vitro results demonstrated that ESW treatment (10 kV, 500 pulses) has a stimulatory effect on the proliferation and osteogenic differentiation of bone marrow-derived MSCs (BMSCs). Histological and micro-CT results showed that PLGA scaffolds seeded with ESW-treated BMSCs produced more bone-like tissue with commitment to the osteogenic lineage when subcutaneously implanted in vivo, as compared to control group. Significantly greater bone formation with a faster mineral apposition rate inside the defect site was observed in the ESW group compared to control group. Biomechanical parameters, including ultimate load and stress at failure, improved over time and were superior to those of the control group. Taken together, this innovative approach shows significant potential in bone tissue regeneration.

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“…Kao et al coated PLA scaffold with polydopamine and noticed enhanced cell adhesion and proliferation on the scaffold [35] . Polydopamine also has sufficient reducing capability for electrodeless plating [34,36] . By dipping the 3D-printed components with a polydopamine coating in the solution containing gold salt, one can easily plate them with gold for the biomolecular self-assembly via gold-thio interactions [37] .…”

Section: Surface Activationmentioning

confidence: 99%

“…Both groups used polydopamine chemistry to immobilize the biologically active materials. Polydopamine is bio-inspired material that has a strong adhesion to virtually any type of surface [34] . The polydopamine coating contains hydroxyl and amine functional groups which could covalently conjugate proteins and other active compounds.…”

Section: Surface Activationmentioning

confidence: 99%

Post-printing surface modification and functionalization of 3D-printed biomedical device

Zhang¹

2024

IJB

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3D printing is a technology well-suited for biomedical applications due to its ability to create highly complex and arbitrary structures from personalized designs with a fast turnaround. However, due to a limited selection of 3D-printable materials, the biofunctionality of many 3D-printed components has not been paid enough attention. In this perspective, we point out that post-3D printing modification is the solution that could close the gap between 3D printing technology and desired biomedical functions. We identify architectural reconfiguration and surface functionalization as the two main post-3D printing modification processes and discuss potential techniques for post-3D printing modification to achieve desired biofunctionality.

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Surface modification of 3D-printed porous scaffolds via mussel-inspired polydopamine and effective immobilization of rhBMP-2 to promote osteogenic differentiation for bone tissue engineering

Cited by 182 publications

References 55 publications

Spatiotemporal control of growth factors in three-dimensional printed scaffolds

Spatiotemporal control of growth factors in three-dimensional printed scaffolds

An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)

Post-printing surface modification and functionalization of 3D-printed biomedical device

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