Surface immobilization of active vascular endothelial growth factor via a cysteine-containing tag

Backer, Marina V.; Patel, Vimal; Jehning, Brian T.; Claffey, Kevin P.; Backer, Joseph M.

doi:10.1016/j.biomaterials.2006.06.025

Cited by 84 publications

(73 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[47,48] Greater control over growth factor orientation has also been accomplished via the use of genetic engineering to introduce tags to the growth factor, such as a cysteinecontaining peptide tag fused to either the N-terminus or Cterminus of VEGF. [49] Oriented, but non-covalent, tethering has also been accomplished following the genetic modification of EGF with multiple types of tags that interact with surfaces functionalized with complementary sequences. [50] Characterization Many options exist for verifying modification of growth factors and their immobilization onto substrates.…”

Section: Other Methodsmentioning

confidence: 99%

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

Masters

2011

Macromolecular Bioscience

161

179

View full text Add to dashboard Cite

Growth factors play a critical role in regulating processes involved in cellular differentiation and tissue regeneration, and are therefore considered essential elements in many tissue engineering strategies. The covalent immobilization of growth factors to biomaterial matrices addresses many of the challenges associated with delivering freely-diffusible growth factors and has thus emerged as a promising method of achieving localized and sustained growth factor delivery. This Feature Article discusses methods that have been used to immobilize growth factors to substrates, followed by an overview of several tissue repair and regeneration applications in which immobilized growth factors have been used.

show abstract

Section: Other Methodsmentioning

confidence: 99%

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

Masters

2011

Macromolecular Bioscience

161

179

View full text Add to dashboard Cite

show abstract

“…Alteration of the scaffold might allow tethering of the injected VEGF, thus protecting it from degradation and enabling a more sustained release. Alternatively, the VEGF could have been tethered by structural molecules such as fibrin in the haematoma and released later to induce neo-vascularisation (Backer et al, 2006;Zisch et al, 2001). In fact, Fig 7 shows quite clearly that VEGF acted synergistically with the cell-seeded dense collagen scaffold to induce bone formation.…”

Section: Discussionmentioning

confidence: 99%

MSC-seeded dense collagen scaffolds with a bolus dose of VEGF promote healing of large bone defects

Gao

Ej²,

Chua³

et al. 2013

eCM

View full text Add to dashboard Cite

The functional repair of large skeletal defects remains a significant challenge to orthopaedic surgeons due to the lack of effective strategies to promote bone regeneration, particularly in the elderly. This study investigated the potential use of bone marrow derived mesenchymal stromal cells (MSC) in a dense collagen scaffold with a bolus dose of vascular endothelial growth factor (VEGF) to repair a defect in the femoral diaphysis of mice. MSC isolated from bone marrow of 4-month-old donor mice were seeded in type I collagen gels that were then compressed to form scaffolds with a fibrillar density similar to osteoid. The cells remained metabolically active in scaffolds incubated in vitro for up to 15 days and differentiated into osteoblasts that deposited calcium-phosphate mineral into the scaffold, which was quantified using micro-computed tomographic (micro-CT) imaging. When implanted in a 1 mm x 3 mm unicortical defect the MSC-loaded scaffolds were rapidly mineralised and integrated into host bone with administration of 10 ng of recombinant VEGF injected into the femoral canal at 4 days postoperative. Empty scaffolds and MSC-seeded scaffolds implanted in defects that did not receive a bolus dose of VEGF did not mineralise or integrate with native bone. The approach with MSC, hydrogels and a biologic factor already approved for human use warrants further pre-clinical investigation with a large animal model.

show abstract

“…When implanted on chicken chorioallantoic membrane, the collagen gels modified with VEGF enhanced capillary formation and tissue ingrowth [47]. VEGF also has been genetically modified to express N-terminal cysteine, and the recombinant protein was conjugated to fibronectin via thiol-directed bifunctional crosslinking reagent without loss in bioactivity [48]. Another well-studied angiogenic growth factor, basic fibroblast growth factor (bFGF), has been incorporated into PEG hydrogels as an immobilized concentration gradient a gradient maker.…”

Section: Modifications With Signalingmentioning

confidence: 99%

Vascularization of Engineered Tissues: Approaches to Promote Angiogenesis in Biomaterials

Moon¹,

West²

2008

CTMC

204

View full text Add to dashboard Cite

Although there have been extensive research efforts to create functional tissues and organs, most successes in tissue engineering have been limited to avascular or thin tissues. The major hurdle in development of more complex tissues lies in the formation of vascular networks capable of delivering oxygen and nutrients throughout the engineered constructs. Sufficient neovascularization in scaffold materials can be achieved through coordinated application of angiogenic factors with proper cell types in biomaterials. This review present the current research developments in the design of biomaterials and their biochemical and biochemical modifications to produce vascularized tissue constructs.

show abstract

Surface immobilization of active vascular endothelial growth factor via a cysteine-containing tag

Cited by 84 publications

References 30 publications

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

MSC-seeded dense collagen scaffolds with a bolus dose of VEGF promote healing of large bone defects

Vascularization of Engineered Tissues: Approaches to Promote Angiogenesis in Biomaterials

Contact Info

Product

Resources

About