Surface functionalized titanium thin films: Zeta-potential, protein adsorption and cell proliferation

Cai, Kaiyong; Frant, Marion; Bossert, Jörg; Hildebrand, Gerhard; Liefeith, Klaus; Jandt, Klaus D.

doi:10.1016/j.colsurfb.2006.03.016

Cited by 243 publications

(164 citation statements)

References 25 publications

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“…As topographical features of bone particles could play a role in the surface interaction with small molecules, we assessed various sizes of particles to scale the specific surface area available for the drugbone surface interaction. Decreasing the micro structural heterogeneity of the bone powder is essential to maintain a uniform surface area for surface reactivity studies [21][22][23]. In addition, the adsorbent properties are functionally dependent on the particle size.…”

Section: Binding Of Raloxifene To Bone Powdermentioning

confidence: 99%

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Bivi

Balagopalakrishna

et al. 2016

Bioorganic & Medicinal Chemistry

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Raloxifene, a selective estrogen receptor modulator (SERM), reduces fracture risk at least in part by improving the mechanical properties of bone in a cell-and estrogen receptor-independent manner. In this study, we determined that raloxifene directly interacts with the bone tissue. Through the use of multiple and complementary biophysical techniques including nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR), we show that raloxifene interacts specifically with the organic component or the organic/mineral composite, and not with hydroxyapatite. Structure-activity studies reveal that the basic side chain of raloxifene is an instrumental determinant in the interaction with bone. Thus, truncation of portions of the side chain reduces bone binding and also diminishes the increase in mechanical properties. Our results support a model wherein the piperidine interacts with bone matrix through electrostatic interactions with the piperidine nitrogen and through hydrophobic interactions (van der Waals) with the aliphatic groups in the side chain and the benzothiopene core. Furthermore, in silico prediction of the potential binding sites on the surface of collagen revealed the presence of a groove with sufficient space to accommodate raloxifene analogs. The hydroxyl groups on the benzothiophene nucleus, which are necessary for binding of SERMs to the estrogen receptor, are not required for binding to the bone surface, but mediate a more robust binding of the compound to the bone powder. In conclusion, we report herein a novel property of raloxifene analogs that allows them to interact with the bone tissue through potential contacts with the organic matrix and in particular collagen.

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Section: Binding Of Raloxifene To Bone Powdermentioning

confidence: 99%

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Bivi

Balagopalakrishna

et al. 2016

Bioorganic & Medicinal Chemistry

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“…. . fuse layer, was detected on bulk samples constructed with the same selective laser melting parameters and hydrothermal conditions [24]. When the nano-functionalized samples were immersed into an aqueous solution, its surface would immediately cover by hydroxyl groups [25].…”

Section: Resultsmentioning

confidence: 96%

Large-scale functionalization of biomedical porous titanium scaffolds surface with TiO2 nanostructures

Qian

Zeng

et al. 2017

Sci. China Mater.

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Construction of functional porous titanium scaffold is drawing ever growing attention, due to its effectiveness in solving the mechanical mismatch between titanium implant and bone tissue. However, the poor water permeability as well as the problem in achieving uniform surface modification inside scaffold hinders the further biomedical application of porous titanium scaffold. In this study, largescale functional TiO 2 nanostructures (nanonetwork, nanoplate and nanowire) were constructed on three-dimensional porous titanium scaffolds surface via an effective hydrothermal treatment method. These nanostructures increase the hydrophilicity of the titanium scaffold surface, facilitating the cell culture medium to penetrate into the inner pore of the scaffold. Zeta potential analyses indicate that the surface electrical properties depend on the nanostructure, with nanowire exhibiting the lowest potential at pH 7.4. The influence of the nano-functionalized scaffold on protein adsorption and cell adhesion was examined. The results indicate that the nano-functionalized surface could modulate protein adsorption and bone marrow derived mesenchymal stem cells (BMSCs) adhesion, with the nanowire functionalized porous scaffold homogeneously promoting protein adsorption and BMSCs adhesion. Our research will facilitate future research on the development of novel functional porous scaffold.

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“…At pH 7.4, ALN results in positive zeta potentials, while RIS results in negative ones as a function of concentration, most likely due to the differing nitrogen charges/species among the two bisphosphonates. (1) Cells may sense and react to this potential, (46)(47)(48)(49)(50)(51)(52)(53) thus it is plausible that the different affinities and zeta-potentials influence osteoblastic function differently, quantitatively and/or qualitatively.…”

Section: Discussionmentioning

confidence: 99%

Effects of alendronate and risedronate on bone material properties in actively forming trabecular bone surfaces

Hofstetter

Gamsjaeger

Phipps³

et al. 2012

Journal of Bone and Mineral Research

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We used Raman and Fourier transform infrared microspectroscopy (FTIRM) analysis to examine the intrinsic bone material properties at actively bone-forming trabecular surfaces in iliac crest biopsies from women with postmenopausal osteoporosis (PMO) who were treated with either alendronate (ALN) or risedronate (RIS). At eight study sites, women were identified who had postmenopausal osteoporosis (PMO), were at least 5 years postmenopause, and had been on long-term therapy (either 3-5 years or >5 years) with daily or weekly ALN or RIS. Following standard tetracycline labeling, biopsies were collected from 102 women (33 treated with ALN for 3-5 years , 35 with ALN for >5 years , 26 with RIS for 3-5 years , and 8 with RIS for >5 years ) and were analyzed at anatomical areas of similar tissue age in bone-forming areas (within the fluorescent double labels). The following outcomes were monitored and reported: mineral to matrix ratio (corresponding to ash weight), relative proteoglycan content (regulating mineralization commencement), mineral maturity (indicative of the mineral crystallite chemistry and stoichiometry, and having a direct bearing on crystallite shape and size), and the ratio of two of the major enzymatic collagen cross-links (pyridinoline/divalent). In RIS-5 there was a significant decrease in the relative proteoglycan content (À5.83% compared to ALN-5), while in both RIS-3 and RIS-5 there was significantly lower mineral maturity/ crystallinity (À6.78% and À13.68% versus ALN-3 and ALN-5, respectively), and pyridinoline/divalent collagen cross-link ratio (À23.09% and À41.85% versus ALN-3 and ALN-5, respectively). The results of the present study indicate that ALN and RIS exert differential effects on the intrinsic bone material properties at actively bone-forming trabecular surfaces. ß

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Surface functionalized titanium thin films: Zeta-potential, protein adsorption and cell proliferation

Cited by 243 publications

References 25 publications

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Large-scale functionalization of biomedical porous titanium scaffolds surface with TiO2 nanostructures

Effects of alendronate and risedronate on bone material properties in actively forming trabecular bone surfaces

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