Surface-enhanced infrared absorption spectroscopy (SEIRAS) to probe monolayers of membrane proteins

Ataka, Kenichi; Stripp, Sven T.; Heberle, Joachim

doi:10.1016/j.bbamem.2013.04.026

Cited by 138 publications

(140 citation statements)

References 62 publications

(67 reference statements)

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…In this context, ultrafast vibrational dynamics in membrane proteins, polymer films or at electrochemical interfaces can be envisioned which have up to now only been studied routinely with comparatively low temporal resolution (approximately nanoseconds). 21,33 In the context of low absorbing samples, possible signal enhancement effects gain significant attraction. In particular Au nanoparticles and roughened surfaces have been widely applied to obtain increased sensitivity in stationary as well as ultrafast spectroscopy.…”

Section: Discussionmentioning

confidence: 99%

“…ATR spectroscopy is usually based on odd orders of (non-)linear susceptibility (χ 1 , χ 3 , etc.) and has been widely used to obtain spectroscopic information from a variety of samples, including self-assembled organic monolayers (MLs) 20 , biological samples 21 or intermediates at electrochemical surfaces 22 . As such, ATR spectroscopy is a method that provides surface-sensitivity and comparatively high signal magnitudes along with a direct comparability between linear and nonlinear spectroscopic results.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Surface-enhanced, multi-dimensional attenuated total reflectance spectroscopy

2015

View full text Add to dashboard Cite

Ultrafast two-dimensional infrared spectroscopy (2D IR) spectroscopy is performed in attenuated total reflectance (ATR) geometry with the Kretschmann configuration in order to measure femtosecond to picosecond dynamics of selfassembled monolayers on gold-coated solid-liquid interfaces. In the monolayers low-absorbing (<200 M-1 cm-1) nitrile functional groups are used as local vibrational probes to monitor vibrational relaxation and spectral diffusion in dependence of different environments of the nitrile group. By comparing spectral diffusion dynamics of the vibrational probe in bulk solution and in the monolayer we find that the dynamics are slowed down by more than a factor of 20 upon immobilization of the sample. Moreover, spectral diffusion dynamics are affected by the local environment within the monolayers as evidenced by 2D ATR IR experiments on mixed monolayers with different aliphatic and aromatic coadsorbates. The results are interpreted in terms of absent excitation energy-transfer as well as solvation dynamics around the nitrile vibrational probe. Our results demonstrate that 2D ATR IR spectroscopy offers the possibility to obtain ultrafast dynamics from sub-monolayer coverages of even low-absorbing vibrational probes such as nitrile functional groups. ABSTRACTUltrafast two-dimensional infrared spectroscopy (2D IR) spectroscopy is performed in attenuated total reflectance (ATR) geometry with the Kretschmann configuration in order to measure femtosecond to picosecond dynamics of selfassembled monolayers on gold-coated solid-liquid interfaces. In the monolayers low-absorbing (<200 M -1 cm -1 ) nitrile functional groups are used as local vibrational probes to monitor vibrational relaxation and spectral diffusion in dependence of different environments of the nitrile group. By comparing spectral diffusion dynamics of the vibrational probe in bulk solution and in the monolayer we find that the dynamics are slowed down by more than a factor of 20 upon immobilization of the sample. Moreover, spectral diffusion dynamics are affected by the local environment within the monolayers as evidenced by 2D ATR IR experiments on mixed monolayers with different aliphatic and aromatic coadsorbates. The results are interpreted in terms of absent excitation energy-transfer as well as solvation dynamics around the nitrile vibrational probe. Our results demonstrate that 2D ATR IR spectroscopy offers the possibility to obtain ultrafast dynamics from sub-monolayer coverages of even low-absorbing vibrational probes such as nitrile functional groups.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface-enhanced, multi-dimensional attenuated total reflectance spectroscopy

2015

View full text Add to dashboard Cite

show abstract

“…Moreover, Ataka and co-authors suggested a reduction of the SEIRA signal to 1/20 upon polarization variation [37]. In our experiment, the estimated reduction factor for the relative intensities of the corresponding TEIRA bands is 1/14.…”

Section: Resultsmentioning

confidence: 53%

“…As the wavelength of infrared radiation is much larger than the distance between metal nanoparticles, the surface optical properties of the metal are strongly dependent on the metal type, immobilized species, or surrounding medium [37]. Conversely, the polarizability of a metal particle is strongly associated with the nanoparticle shape and state of the polarization of the incident radiation [8].…”

Section: Introductionmentioning

confidence: 99%

Polarization effect in tip-enhanced infrared nanospectroscopy studies of the selective Y5 receptor antagonist Lu AA33810

et al. 2018

View full text Add to dashboard Cite

A novel approach of combining conventional infrared spectroscopy (IR) and atomic force microscopy (AFM) is presented to better understand the behavior of a drug adsorbed on a metal substrate at the nanoscale level. Tip-enhanced infrared nanospectroscopy (TEIRA) was used for the first time to investigate Lu AA33810, a selective brain-penetrating Y5 receptor antagonist, after immobilization on gold nanoparticles (GNPs). Here, a gold coated AFM tip and gold substrate were used to obtain the near-field electromagnetic field trapping effect. Because of the huge signal enhancement, it was possible to obtain the spectral information regarding the self-assembled monolayer of the investigated molecule. The effect of two orthogonal polarizations (p-and s-polarization modulations) of the excitation laser beam on the spectral patterns is also discussed. The results show that there is a strong relationship between the state of polarization of the incident radiation and the relative infrared band intensities. Another factor affecting the observed spectral differences is the topology of the metal substrate, which may result in the induction of a cross-polarization effect. The performed analysis indicates that the C-C bond from the cyclohexyl group is oriented almost parallel to the metal surface. Conversely, the p-and s-polarized spectral variations suggest that the O=S=O angle is high enough to enable the simultaneous interaction of both oxygen atoms with the GNPs.

show abstract

“…In spectrum, the parallel dipole cannot be detected while the perpendicular dipole signal is enhanced. This is responsible for some abnormal IR enhancement of the adsorbed molecules in ATR-SEIRAS compared with non-adsorbed molecules, which is also useful to distinguish molecule orientation on metal surfaces [24].…”

Section: Enhancement Mechanismmentioning

confidence: 99%

Attenuated Total Reflection Surface-Enhanced Infrared Absorption Spectroscopy: a Powerful Technique for Bioanalysis

Zhang

et al. 2017

J. Anal. Test.

View full text Add to dashboard Cite

Attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) has recently been proven to be a powerful tool for bioanalysis. It enables in situ and in real-time observation of dynamic processes occurring on specific interface, revealing rich structural and functional information of biomolecules at sub monolayer level. The aim of this general review was to give an overview of the cutting edge applications of ATR-SEIRAS. We start with description of the basic configuration of the standard ATR-SEIRAS platform. The enhanced mechanisms and methods to fabricate enhanced substrates are then presented. We discuss the recent developments, challenges and applications of ATR-SEIRAS in bioanalysis, mainly focusing on DNA analysis, protein behavior and cell properties. Finally, further development of the ATR-SEIRAS technique with enhanced sensitivity, improved time and spatial resolutions will be prospected.

show abstract

Surface-enhanced infrared absorption spectroscopy (SEIRAS) to probe monolayers of membrane proteins

Cited by 138 publications

References 62 publications

Surface-enhanced, multi-dimensional attenuated total reflectance spectroscopy

Surface-enhanced, multi-dimensional attenuated total reflectance spectroscopy

Polarization effect in tip-enhanced infrared nanospectroscopy studies of the selective Y5 receptor antagonist Lu AA33810

Attenuated Total Reflection Surface-Enhanced Infrared Absorption Spectroscopy: a Powerful Technique for Bioanalysis

Contact Info

Product

Resources

About