2023
DOI: 10.1016/j.jconrel.2023.04.033
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Surface display of functional moieties on extracellular vesicles using lipid anchors

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Cited by 9 publications
(6 citation statements)
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“…Since EVs are mixtures of endosome-and plasma membranederived lipid vesicles, their negative surface potential and the presence of anionic lipids, such as phosphatidylserine, could in a similar way induce structural rearrangements within L3-based LCNPs (71,72). Furthermore, recent findings suggest that particularly DOPE interacts strongly with EV membranes, highlighting its important role in the hybridization process (73). NanoFCM revealed that various NA molecules (i.e., mRNA, siRNA and RNA aptamer) could be incorporated into EVs, highlighting the versatility of the LCNP platform for loading EVs.…”
Section: Discussionmentioning
confidence: 99%
“…Since EVs are mixtures of endosome-and plasma membranederived lipid vesicles, their negative surface potential and the presence of anionic lipids, such as phosphatidylserine, could in a similar way induce structural rearrangements within L3-based LCNPs (71,72). Furthermore, recent findings suggest that particularly DOPE interacts strongly with EV membranes, highlighting its important role in the hybridization process (73). NanoFCM revealed that various NA molecules (i.e., mRNA, siRNA and RNA aptamer) could be incorporated into EVs, highlighting the versatility of the LCNP platform for loading EVs.…”
Section: Discussionmentioning
confidence: 99%
“…DOPE is a common phospholipid anchor used for membrane surface modification, 19 and DOPE-polymers have been often used for exosome modification based on a similar mechanism of DOPE serving as an anchor and inserting in the membrane. 20 The heparin/ACE2 comodified extracellular vesicles (the haEVs) were prepared by coincubating aEVs and DOPE-Hp via a self-assembly process in which DOPE was inserted into the aEVs membrane. Figure S2A shows that the surface modification efficiency of DOPE-Hp reached a stable level at 5 mg/mL.…”
Section: ■ Preparation and Characterization Of Heparin/ace2 Comodifie...mentioning
confidence: 99%
“…Cholesterol is also one component of EV membrane, which spontaneously inserts into the membrane via its hydrophobic moiety ( 94 ), and cholesterol-modified RNA structures can be displayed on the EV surface that improves the efficiency of delivery of the modified EVs ( 68 ). Similarly, ceramide and dioleoyl phosphatidylethanolamine (DOPE) are being explored for their lipid anchoring properties with DOPE showing the most efficient anchoring effect and stable retention ( 95 ).…”
Section: Ev Tropism Is Demonstrated By Recent Examplesmentioning
confidence: 99%