Surface curvature in triply-periodic minimal surface architectures as a distinct design parameter in preparing advanced tissue engineering scaffolds

Blanquer, Sébastien; Werner, Maike; Hannula, Markus; Sharifi, Shahriar; Lajoinie, Guillaume; Eglin, David; Hyttinen, Jari; Poot, Andreas A.; Grijpma, Dirk W.

doi:10.1088/1758-5090/aa6553

Cited by 123 publications

(66 citation statements)

References 49 publications

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“…While we have so far focused on fiber‐ or tube‐like cylindrical structures, where k ≥ 0 (convex) or k ≤ 0 (concave), more complex geometries in biomaterials and scaffolds can concurrently present both positive and negative curvatures to the cells . Thus, we next studied structures that contain both convex and concave surfaces in different directions.…”

Section: Resultsmentioning

confidence: 99%

Cell‐Perceived Substrate Curvature Dynamically Coordinates the Direction, Speed, and Persistence of Stromal Cell Migration

et al. 2019

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Adherent cells residing within tissues or biomaterials are presented with 3D geometrical cues from their environment, often in the form of local surface curvatures. While there is growing evidence that cellular decision‐making is influenced by substrate curvature, the effect of physiologically relevant, cell‐scale anisotropic curvatures remains poorly understood. This study systematically explores the migration behavior of human bone marrow stromal cells (hBMSCs) on a library of anisotropic curved structures. Analysis of cell trajectories reveals that, on convex cylindrical structures, hBMSC migration speed and persistence are strongly governed by the cellular orientation on the curved structure, while migration on concave cylindrical structures is characterized by fast but non‐aligned and non‐persistent migration. Concurrent presentation of concave and convex substrates on toroidal structures induces migration in the direction where hBMSCs can most effectively avoid cell bending. These distinct migration behaviors are found to be universally explained by the cell‐perceived substrate curvature, which on anisotropic curved structures is dependent on both the temporally varying cell orientation and the 3D cellular morphology. This work demonstrates that cell migration is dynamically guided by the perceived curvature of the underlying substrate, providing an important biomaterial design parameter for instructing cell migration in tissue engineering and regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Cell‐Perceived Substrate Curvature Dynamically Coordinates the Direction, Speed, and Persistence of Stromal Cell Migration

et al. 2019

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“…Using the mathematical formulas described by [47,48] cylindrical porous 3D structures with a diamond pore architecture were generated using Mathmod 3.1 (https://sourceforge.net/projects/mathmod/). Subsequently these structures were modified to have a diameter of approximately 10mm and a height of 7.5-8 mm and converted into printable .stl files using CAD software (Rhinoceros 3D, Robert McNeel and associates).…”

Section: Stereolithographymentioning

confidence: 99%

Drug-releasing biopolymeric structures manufactured via stereolithography

Asikainen¹,

Bochove²,

Seppälä³

2019

Biomed. Phys. Eng. Express

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Additive manufacturing (AM) techniques, such as stereolithography (SLA), enable the preparation of designed complex structures. AM has gained interest especially in the tissue engineering field due to the possibility to manufacture patient specific implants. However, AM could be useful also in controlled drug release applications, since the size and shape of the device, pore architecture and surface to volume ratio can be accurately designed. In this study, SLA was used to prepare polycaprolactone scaffold structures containing the model drug lidocaine. The release of lidocaine was studied and the influence of porosity and surface to volume ratio of structures to the drug release was analyzed. Porous samples released lidocaine faster compared to solid ones, whereas the degree of porosity and surface to volume ratio did not have a clear effect on the drug release profile.

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“…The external geometry of the scaffold can be designed in a patient-specific manner, based on medical imaging data (e.g., from MRI or CT scans) [17]. Moreover, this approach also allows detailed control of the internal geometry, even using mathematically defined functions [19,28,29]. Reproducible control of the internal scaffold architecture plays an essential role in employing the scaffold internal design to guide cell infiltration and tissue organization in the desired direction.…”

Section: Introductionmentioning

confidence: 99%

“…The Gaussian curvature distribution of the scaffold struts can be calculated and by adjusting the number of unit cells and scaffold dimensions a scaffold can be designed with predefined Gaussian curvature distributions. Image adapted from [28]. (D) The CAD model of a hierarchical scaffold that combines gel microfibers (20 wt % gelatin solution and 4 wt % sodium alginate, diameter = 440 µm) produced by 3D bioprinting and PCL nanofibers (diameter = 190 nm) produced by electrospinning.…”

Section: Introductionmentioning

confidence: 99%

Cellular Geometry Sensing at Different Length Scales and its Implications for Scaffold Design

2020

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Geometrical cues provided by the intrinsic architecture of tissues and implanted biomaterials have a high relevance in controlling cellular behavior. Knowledge of how cells sense and subsequently respond to complex geometrical cues of various sizes and origins is needed to understand the role of the architecture of the extracellular environment as a cell-instructive parameter. This is of particular interest in the field of tissue engineering, where the success of scaffold-guided tissue regeneration largely depends on the formation of new tissue in a native-like organization in order to ensure proper tissue function. A well-considered internal scaffold design (i.e., the inner architecture of the porous structure) can largely contribute to the desired cell and tissue organization. Advances in scaffold production techniques for tissue engineering purposes in the last years have provided the possibility to accurately create scaffolds with defined macroscale external and microscale internal architectures. Using the knowledge of how cells sense geometrical cues of different size ranges can drive the rational design of scaffolds that control cellular and tissue architecture. This concise review addresses the recently gained knowledge of the sensory mechanisms of cells towards geometrical cues of different sizes (from the nanometer to millimeter scale) and points out how this insight can contribute to informed architectural scaffold designs.

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Surface curvature in triply-periodic minimal surface architectures as a distinct design parameter in preparing advanced tissue engineering scaffolds

Cited by 123 publications

References 49 publications

Cell‐Perceived Substrate Curvature Dynamically Coordinates the Direction, Speed, and Persistence of Stromal Cell Migration

Cell‐Perceived Substrate Curvature Dynamically Coordinates the Direction, Speed, and Persistence of Stromal Cell Migration

Drug-releasing biopolymeric structures manufactured via stereolithography

Cellular Geometry Sensing at Different Length Scales and its Implications for Scaffold Design

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