Surface Charge Measurement of SonoVue, Definity and Optison: A Comparison of Laser Doppler Electrophoresis and Micro-Electrophoresis

Ja’afar, Fairuzeta; Leow, Chee Hau; Garbin, Valeria; Sennoga, Charles A.; Tang, Meng‐Xing; Seddon, John M.

doi:10.1016/j.ultrasmedbio.2015.07.001

Cited by 28 publications

(28 citation statements)

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“…All of the commercial UCAs listed in Table 1 have an anionic surface charge [40]. The charged surface results in an enriched “double layer” of counter ions in the intervening medium that yields an osmotic repulsion, whose interaction energy for two spheres is given by [45]: Wes(x)=12RZe−κdx…”

Section: Properties Of the Ucamentioning

confidence: 99%

“…Figure 3b shows the theoretical interaction potential between two 3-μm diameter Optison bubbles based on the equations above. The surface of Optison is negatively charged (ψ0=−9.5mV) [40], but it is the hydration force that provides stability.…”

Section: Properties Of the Ucamentioning

confidence: 99%

“… DPPC = 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DPPA = 1,2-dipalmitoyl- sn -glycero-3-phosphate; DSPC = 1,2-distearoyl-sn-glycero-3-phosphocholine; DPPE-mPEG 5000 = 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000]; DPPG = 1,2-dipalmitoyl- sn -glycero-3-phospho-(1’- rac -glycerol); PA = palmitic acid; 𝜓 ₀ is the zeta potential measured [40]. …”

Section: Figurementioning

confidence: 99%

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Reverse engineering the ultrasound contrast agent

Borden

Song

2018

Advances in Colloid and Interface Science

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In this review, a brief history and current state-of-the-art is given to stimulate the rational design of new microbubbles through the reverse engineering of current ultrasound contrast agents (UCAs). It is shown that an effective microbubble should be biocompatible, echogenic and stable. Physical mechanisms and engineering calculations have been provided to illustrate these properties and how they can be achieved. The rational design paradigm is applied to study current FDA-approved and commercially available UCAs. Given the sophistication of microbubble designs reported in the literature, rapid development and adoption of ultrasound device hardware and the growing number of revolutionary biomedical applications moving toward the clinic, the field of Microbubble Engineering is fertile for breakthroughs in next- generation UCA technology. It is up to current and future microbubble engineers and clinicians to push forward with regulatory approval and clinical adoption of advanced UCA technologies in the years to come.

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Section: Properties Of the Ucamentioning

confidence: 99%

Section: Properties Of the Ucamentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Reverse engineering the ultrasound contrast agent

Borden

Song

2018

Advances in Colloid and Interface Science

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“…However, the main limitation of this method is that the buoyancy force acting on the bubbles significantly affects their measurement as commercial systems typically have a vertical. An alternative approach reported in [109] uses a simple microfluidic device for obtaining the zeta potential of three types of commercial microbubbles. The microbubbles are suspended in a horizontal microchannel under an electrical field and optically tracked as illustrated in Fig.…”

Section: B Quantification Of Loading Capacity 1) Direct Incorporationmentioning

confidence: 99%

Characterization of Contrast Agent Microbubbles for Ultrasound Imaging and Therapy Research

Mulvana

Browning

Luan

et al. 2017

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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The high efficiency with which gas microbubbles can scatter ultrasound compared with the surrounding blood pool or tissues has led to their widespread employment as contrast agents in ultrasound imaging. In recent years, their applications have been extended to include super-resolution imaging and the stimulation of localized bio-effects for therapy. The growing exploitation of contrast agents in ultrasound and in particular these recent developments have amplified the need to characterize and fully understand microbubble behavior. The aim in doing so is to more fully exploit their utility for both diagnostic imaging and potential future therapeutic applications. This paper presents the key characteristics of microbubbles that determine their efficacy in diagnostic and therapeutic applications and the corresponding techniques for their measurement. In each case, we have presented information regarding the methods available and their respective strengths and limitations, with the aim of presenting information relevant to the selection of appropriate characterization methods. First, we examine methods for determining the physical properties of microbubble suspensions and then techniques for acoustic characterization of both suspensions and single microbubbles. The next section covers characterization of microbubbles as therapeutic agents, including as drug carriers for which detailed understanding of their surface characteristics and drug loading capacity is required. Finally, we discuss the attempts that have been made to allow comparison across the methods employed by various groups to characterize and describe their microbubble suspensions and promote wider discussion and comparison of microbubble behavior.

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“…Furthermore, the PLNPs-RSG were modified by coupling with PEI to fabricate cationic PLNPs-RSG. In addition, freeze-dried commercial SonoVue powder coated with a thin lipid monolayer membrane shell and containing sulfur hexafluoride-filled MBs was used, as the surface charge of SonoVue MBs is negative, 23 the cationic PLNPs-RSG were conjugated with the MBs to synthesize a novel PLNPs-RSG-MBs complex via electrostatic interactions ( Figure 1A ) with a conjugation method similar to the protocol described in a previous study. 24 Furthermore, this complex was administered to a unilateral ureteral obstruction (UUO) rat model with the assistance of US exposure, and the purpose of this study was to investigate its antifibrotic effect on RIF ( Figure 1B ).…”

Section: Introductionmentioning

confidence: 99%

<p>Ultrasound Assisted a Peroxisome Proliferator-Activated Receptor (PPAR)γ Agonist-Loaded Nanoparticle-Microbubble Complex to Attenuate Renal Interstitial Fibrosis</p>

Wei

Zhang

et al. 2020

IJN

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Objective: To investigate the antifibrotic effect of the combination of a PPARγ agonistloaded nanoparticle-microbubble complex with ultrasound (US) exposure on renal interstitial fibrosis (RIF). Materials and Methods: Polylactide-co-glycolide (PLGA) nanoparticles were used to load PPARγ agonist (rosiglitazone, RSG) and prepare PLGA-RSG nanoparticles (PLNPs-RSG); then, a novel complex between PLNPs-RSG and SonoVue microbubbles (MBs) (PLNPs-RSG-MBs) was prepared. The size distribution, zeta potentials, RSG-loading capacity and entrapment efficiency were measured, and the release of RSG was assessed using a UV-vis spectrophotometer. The in vitro cytotoxicity and in vivo systemic toxicity assays were performed. The cellular uptake assessment was performed using a confocal laser scanning microscope (CLSM). The in vivo biodistribution assessment was performed using fluorescence imaging with a near-infrared (NIR) imaging system. Furthermore, this complex was administered to a unilateral ureteral obstruction (UUO) rat model with the assistance of US exposure to investigate the antifibrotic effect. Results: This PLNPs-RSG-MBs complex had a size of 2199.5± 988.1 nm and a drugloading efficiency of 28.5%. In vitro cytotoxicity and in vivo systemic toxicity assays indicated that the PLNPs-RSG-MBs complex displayed excellent biocompatibility. In addition, the complex showed high cellular uptake efficiency in vitro and kidney-targeting ability in vivo. In a UUO rat model, the combination of the PLNPs-RSG-MBs complex with US exposure significantly reduced collagen deposition and successfully attenuated renal fibrosis. Conclusion: The combination of the PLNPs-RSG-MBs complex with US exposure may be a promising approach for the treatment of RIF.

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Surface Charge Measurement of SonoVue, Definity and Optison: A Comparison of Laser Doppler Electrophoresis and Micro-Electrophoresis

Cited by 28 publications

References 13 publications

Reverse engineering the ultrasound contrast agent

Reverse engineering the ultrasound contrast agent

Characterization of Contrast Agent Microbubbles for Ultrasound Imaging and Therapy Research

<p>Ultrasound Assisted a Peroxisome Proliferator-Activated Receptor (PPAR)γ Agonist-Loaded Nanoparticle-Microbubble Complex to Attenuate Renal Interstitial Fibrosis</p>

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