Surface charge effect on mucoadhesion of chitosan based nanogels for local anti-colorectal cancer drug delivery

Feng, Chao; Li, Jing; Kong, Ming; Liu, Ya; Cheng, Xiao; Li, Yang; Park, Hyun Jin; Chen, Xi Guang

doi:10.1016/j.colsurfb.2015.02.042

Cited by 111 publications

(37 citation statements)

References 40 publications

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“…We were successful in encapsulating the purified rTcdB with these two components to form double-layered nanoparticles with sizes ranging from 200 to 750 nm in diameter. Surface charge is one of the most important factors affecting the function of nanoparticles in adhering and transporting across the intestinal epithelial cells (Frohlich, 2012; Feng et al, 2015). Positively charged nanoparticles are easier to be taken up by cells than negatively charged ones (Chen L. et al, 2011; Yue et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Immunization with Recombinant TcdB-Encapsulated Nanocomplex Induces Protection against Clostridium difficile Challenge in a Mouse Model

Liu

Chen

et al. 2017

Front. Microbiol.

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Clostridium difficile is considered to be one of the major cause of infectious diarrhea in healthcare systems worldwide. Symptoms of C. difficile infection are caused largely by the production of two cytotoxins: toxin A (TcdA) and toxin B (TcdB). Vaccine development is considered desirable as it would decrease the mounting medical costs and mortality associated with C. difficile infections. Biodegradable nanoparticles composed of poly-γ-glutamic acid (γ-PGA) and chitosan have proven to be a safe and effective antigen delivery system for many viral vaccines. However, few studies have used this efficient antigen carrier for bacterial vaccine development. In this study, we eliminated the toxin activity domain of toxin B by constructing a recombinant protein rTcdB consists of residues 1852-2363 of TcdB receptor binding domain. The rTcdB was encapsulated in nanoparticles composed of γ-PGA and chitosan. Three rounds of intraperitoneal vaccination led to high anti-TcdB antibody responses and afforded mice full protection mice from lethal dose of C. difficile spore challenge. Protection was associated with high levels of toxin-neutralizing antibodies, and the rTcdB-encapsulated NPs elicited a longer-lasting antibody titers than antigen with the conventional adjuvant, aluminum hydroxide. Significant reductions in the level of proinflammatory cytokines and chemokines were observed in vaccinated mouse. These results suggested that polymeric nanocomplex-based vaccine design can be useful in developing vaccine against C. difficile infections.

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Section: Discussionmentioning

confidence: 99%

Immunization with Recombinant TcdB-Encapsulated Nanocomplex Induces Protection against Clostridium difficile Challenge in a Mouse Model

Liu

Chen

et al. 2017

Front. Microbiol.

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“…No significant differences are observed when unloaded batches are compared with batches containing any drug, so it can be concluded that the addition of drugs to the discs barely modifies their mucoadhesive properties (Table 4). The fact that the addition of SDS causes the system to remain adhered until its complete dissolution is because it is an anionic surfactant that enables the discs to bond to the mucosa due to electrostatic charges and with hydrogen bonds formed by HPMC [64,65]. In contrast, the inclusion of 2HPβCD scarcely modifies the disc structure so it also retains most of the adhesive potential of HPMC; these batches have the second longest mucoadhesion time after the reference batches.…”

Section: Mucoadhesion Time and Forcementioning

confidence: 99%

Mucoadhesive Vaginal Discs based on Cyclodextrin and Surfactants for the Controlled Release of Antiretroviral Drugs to Prevent the Sexual Transmission of HIV

et al. 2020

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The strategies for developing vaginal microbicides to protect women against human immunodeficiency virus (HIV) sexual transmission are constantly changing. Although the initial dosage forms required daily administration to offer effective protection, the trend then moved towards sustained-release dosage forms that require less frequency of administration in order to improve women’s compliance with the treatment. Nevertheless, another possible strategy is to design on-demand products that can be used in a coitally-dependent manner and only need to be administered immediately before intercourse to offer protection. Vaginal discs based on freeze-dried hydroxypropylmethyl cellulose gels have been developed for this purpose, containing two surfactants, i.e., sodium dodecyl sulphate and polysorbate 60, alone or in combination with 2-hydroxypropyl-β-cyclodextrin, to achieve a formulation capable of incorporating both hydrophilic and lipophilic drugs. Several studies have been carried out to evaluate how the inclusion of these substances modifies the structure of gels (viscosity and consistency studies) and the porosimetry of the freeze-dried discs (scanning electron microscopy micrographs, mechanical properties, swelling behaviour). The drug release and mucoadhesive properties of the discs have also been evaluated with a view to their clinical application. The systems combining sodium dodecyl sulphate and 2-hydroxypropyl-β-cyclodextrin were found to be adequate for the vaginal administration of both Tenofovir and Dapivirine and also offer excellent mucoadhesion to vaginal tissue; these discs could therefore be an interesting option for a coitally-dependent administration to protect women against HIV transmission.

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“…Muzzarelli et al [73] proposed the use of N-CMCS in cosmetic products for buccal applications such as toothpastes, mouthwashes, gingival gels, or artificial saliva formulations. The use of this polymer in buccal products has been explored because of its mucoadhesive properties [74,75].…”

Section: Use In Cosmeticsmentioning

confidence: 99%

An overview of carboxymethyl derivatives of chitosan: Their use as biomaterials and drug delivery systems

Fonseca-Santos

2017

Materials Science and Engineering: C

215

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Chitin is one of the most abundant natural polymers in the world and is used for the production of chitosan by deacetylation. Chitosan is nontoxic and biodegradable and, therefore, can be used as a biomaterial and for the construction of drug delivery systems. Nevertheless, the poor solubility of chitosan in neutral or alkalinized media has restricted its applications in the pharmaceutical and biomedical fields. Chitosan can be easily carboxymethylated to improve its solubility in aqueous media while its biodegradability and biocompatibility are preserved. Carboxymethyl chitosans show improved solubility in aqueous media, which makes them an attractive alternative source for producing biomaterials and drug delivery systems as well as for designing nanotechnology-based systems. Thus, carboxymethyl chitosan-based materials have a wide applicability and good potential in the development of biomedical nanodevices and controlled release drug formulations. This review summarizes preparations and properties of hydrophilic chitosan-based materials such as nanoparticles, microparticles, tablets, and films as well as procedures related to various practical applications.

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Surface charge effect on mucoadhesion of chitosan based nanogels for local anti-colorectal cancer drug delivery

Cited by 111 publications

References 40 publications

Immunization with Recombinant TcdB-Encapsulated Nanocomplex Induces Protection against Clostridium difficile Challenge in a Mouse Model

Immunization with Recombinant TcdB-Encapsulated Nanocomplex Induces Protection against Clostridium difficile Challenge in a Mouse Model

Mucoadhesive Vaginal Discs based on Cyclodextrin and Surfactants for the Controlled Release of Antiretroviral Drugs to Prevent the Sexual Transmission of HIV

An overview of carboxymethyl derivatives of chitosan: Their use as biomaterials and drug delivery systems

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