Surface Characterization of Biopolymer Micropatterns Processed by Ion‐Beam Modification and PECVD

Manso‐Silván, Miguel; Valsesia, Andrea; Hasiwa, Marina; Gilliland, Douglas; Ceccone, Giacomo; Rossi, François

doi:10.1002/cvde.200606580

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…Helium beam exposure renders the grafted PEG on the surface less hydrophilic by a decrease in C–O ether and an increase in C–C alkyl functionalities, and thus potentially more amenable to protein adsorption. There also is literature evidence that electron [9] or ion (argon) beam [10], [11] exposure can create carbonyl functionalities (which could be charged carboxylate or protein amine-aldehyde) in PEG samples. A relatively small number of these functional groups could alter the local protein-capture properties of the PRG surface, while being difficult to detect against the background of much-more-numerous ether and alkyl functionalities.…”

Section: Resultsmentioning

confidence: 99%

“…The amine groups were then used to attach molecules (carboxylic acid anhydrides and rhodamine dyes) to the exposed regions of the surface. Perhaps the most popular approach, however, involves the use of poly(ethylene glycol) (PEG) [9], [10], [11], which is well-known for its resistance to non-specific adsorption of proteins through a combination of entropic (steric stabilization) and enthalpic (hydrogen bonding) mechanisms [12], [13]. Entropic passivation is favored by longer chains which can assume a greater variety of configurations [13], [14], [15], but even smaller ethylene oxide chains of 3–6 monomer units can resist protein adsorption as long as the molecular conformation is helical or amorphous, favoring a more stable interfacial layer of tightly-bound water [12], [13], [15], [16], [17], [18].…”

Section: Introductionmentioning

confidence: 99%

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High-Resolution, High-Throughput, Positive-Tone Patterning of Poly(ethylene glycol) by Helium Beam Exposure through Stencil Masks

et al. 2013

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In this work, a collimated helium beam was used to activate a thiol-poly(ethylene glycol) (SH-PEG) monolayer on gold to selectively capture proteins in the exposed regions. Protein patterns were formed at high throughput by exposing a stencil mask placed in proximity to the PEG-coated surface to a broad beam of helium particles, followed by incubation in a protein solution. Attenuated Total Reflectance–Fourier Transform Infrared Spectroscopy (ATR–FTIR) spectra showed that SH-PEG molecules remain attached to gold after exposure to beam doses of 1.5–60 µC/cm2 and incubation in PBS buffer for one hour, as evidenced by the presence of characteristic ether and methoxy peaks at 1120 cm−1 and 2870 cm−1, respectively. X-ray Photoelectron Spectroscopy (XPS) spectra showed that increasing beam doses destroy ether (C–O) bonds in PEG molecules as evidenced by the decrease in carbon C1s peak at 286.6 eV and increased alkyl (C–C) signal at 284.6 eV. XPS spectra also demonstrated protein capture on beam-exposed PEG regions through the appearance of a nitrogen N1s peak at 400 eV and carbon C1s peak at 288 eV binding energies, while the unexposed PEG areas remained protein-free. The characteristic activities of avidin and horseradish peroxidase were preserved after attachment on beam-exposed regions. Protein patterns created using a 35 µm mesh mask were visualized by localized formation of insoluble diformazan precipitates by alkaline phosphatase conversion of its substrate bromochloroindoyl phosphate-nitroblue tetrazolium (BCIP-NBT) and by avidin binding of biotinylated antibodies conjugated on 100 nm gold nanoparticles (AuNP). Patterns created using a mask with smaller 300 nm openings were detected by specific binding of 40 nm AuNP probes and by localized HRP-mediated deposition of silver nanoparticles. Corresponding BSA-passivated negative controls showed very few bound AuNP probes and little to no enzymatic formation of diformazan precipitates or silver nanoparticles.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-Resolution, High-Throughput, Positive-Tone Patterning of Poly(ethylene glycol) by Helium Beam Exposure through Stencil Masks

et al. 2013

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“…[17][18][19] For instance, contrasted surfaces with bioadhesive and non-bioadhesive areas allow an accurate spatial control of protein adhesion. [20][21][22] Numerous methods for producing micro and nano-patterned surfaces have been developed. [23] Among them, electron beam lithography (EBL) stands out due to its capacity for reproducing structured surfaces with well-defined geometries.…”

Section: Introductionmentioning

confidence: 99%

“…Besides, surfaces exhibiting chemical contrasts represent a platform of choice for optimizing the biomolecule–surface interactions . For instance, contrasted surfaces with bioadhesive and non‐bioadhesive areas allow an accurate spatial control of protein adhesion . Numerous methods for producing micro and nano‐patterned surfaces have been developed .…”

Section: Introductionmentioning

confidence: 99%

Interactions of Serum Derived Proteins with Sub‐Micrometer Structured Surfaces

Perez-Roldan

Parracino

Ceccone

et al. 2014

Plasma Processes & Polymers

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This work presents the development and optimization of two fabrication methods that combine plasma polymerization and electron beam lithography techniques for producing chemical patterns at the sub-micro scale. The first method uses sacrificial resist as mask to produce the functionalization of the bioadhesive areas and the second method consists in the direct EBL writing of adhesive regions on a non-adhesive plasma deposited PEO-like film. The produced patterned surfaces exhibit high binding capacity as tested by Surface Plasmon Resonance Imaging with Human Serum Albumin. Furthermore, we show that adsorption on the structured surfaces is similar to that observed on a flat surface with a direct proportionality to the active area. This work shows the efficiency and suitability of the developed chemical patterning techniques and their high potential applicability for the study of protein surface interactions and miniaturized biosensing device development.

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Improving the sensitivity of immunoassays with PEG‐COOH‐like film prepared by plasma‐based technique

O’Mahony

Gubala

Gandhiraman

et al. 2011

J Biomedical Materials Res

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Herein we report on a preparation and performance of stable, hydrophilic and biocompatible polymeric material suitable for functionalization of disposable substrates used in biosensors. This new material features ACOOH surface groups cross-linked with ethylene glycol molecules and was prepared in situ on disposable, plastic substrate by high-throughput and environmentally friendly technique called plasma-enhanced chemical vapor deposition (PECVD). The film is grafted to the plasma activated plastic by sequential deposition of tetraethylorthosilicate, forming a bonding layer, and mixed vapors of acrylic acid and diethyleneglycol dimethylether (AA/PEG) that provide the desired functional groups forming a sensing, contact layer. A superior performance of the AA/PEG coating as suitable material for substrates in biomedical devices was demonstrated in a model fluorescence linked immunosorbent assay. The results were compared with other commonly used surface materials prepared by wet chemistry methods. The unique characteristic of the AA/PEG film is that the immunoassay can be executed without the need for a blocking step, typically using albumins, without negative consequences on the bioassay results. In fact, the superior quality of the materials modified with AA/PEG film was highlighted by improving the sensitivity of an immunoassay by two orders of magnitude when compared with substrates prepared by standard surface chemistry methods.

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Surface Characterization of Biopolymer Micropatterns Processed by Ion‐Beam Modification and PECVD

Cited by 8 publications

References 34 publications

High-Resolution, High-Throughput, Positive-Tone Patterning of Poly(ethylene glycol) by Helium Beam Exposure through Stencil Masks

High-Resolution, High-Throughput, Positive-Tone Patterning of Poly(ethylene glycol) by Helium Beam Exposure through Stencil Masks

Interactions of Serum Derived Proteins with Sub‐Micrometer Structured Surfaces

Improving the sensitivity of immunoassays with PEG‐COOH‐like film prepared by plasma‐based technique

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