Surface-attached polymer monolayers for the control of endothelial cell adhesion

Chang, Bong‐Jun; Prucker, Oswald; Groh, E; Wallrath, Anja; Dahm, Manfred; Rühe, Jürgen

doi:10.1016/s0927-7757(01)00952-9

Cited by 40 publications

(34 citation statements)

References 19 publications

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“…This extends the work of Chang et al who have shown that primary sheep and rat fibrocytes and HUVECs will grow on PEtOx covalently attached to glass [24]. In the present study, immunofluorescent staining of the confluent cell layers showed 16HBE cells had formed tight junctions and that the fibroblasts had well organised actin filaments, suggesting that the structural organization of the cells was not affected by growth on the polymers.…”

Section: Discussionsupporting

confidence: 90%

“…The side chain can be easily modified with amines [22] or carboxylic acids [23] thereby allowing for further enhancement of the surface via peptide conjugation or other functionalization. For cell adhesive surfaces it has been shown that attachment of poly(2-ethyl-2-oxazoline) (PEtOx) to glass allows the growth of human umbilical vein endothelial cells (HUVECs) and primary rat and sheep fibrocytes [24]; HUVECs can also adhere and spread on fibronectin-coated PEtOx and poly(2-methyl-2-oxazoline) PMeOx attached to glass [19]. Very little else is known about the biocompatibility of related oxazoline polymers, in particular their utility for supporting cell growth or their selectivity for different cell types.…”

Section: Introductionmentioning

confidence: 99%

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Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines

et al. 2015

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Development of synthetic surfaces that are highly reproducible and biocompatible for in vitro cell culture offers potential for development of improved models for studies of cellular physiology and pathology. They may also be useful in tissue engineering by removal of the need for biologically-derived components such as extracellular matrix proteins. We synthesised four types of 2-alkyl-2-oxazoline polymers ranging from the hydrophilic poly(2-methyl-2-oxazoline) to the hydrophobic poly(2-n-butyl-2-oxazoline). The polymers were terminated using amine-functionalised glass coverslips, enabling the synthetic procedure to be reproducible and scaleable. The polymer-coated glass slides were tested for biocompatibility using human epithelial (16HBE 14o-) and fibroblastic (MRC5) cell lines.Differences in adhesion and motility of the two cell types was observed, with the poly(2-isopropyl-2-oxazoline) polymer equally supporting the growth of both cell types, whereas poly(2-n-butyl-2-oxazoline) showed selectivity for fibroblast growth. In summary, 2-alkyl-2-oxazoline polymers may be a useful tool for building in vitro model cell culture models with preferential adhesion of specific cell types.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines

et al. 2015

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“…With a layer thickness of at least 50 nm, almost all the tested bacteria comprising Escherichia coli and Staphylococcus aureus were killed. The alternative approach was reported as well [218]: pEtOx, pEI and various other polymers were covalently bound to surfaces modified by a benzophenone monolayer using a photochemical process. For the attachment of the polymers via their C-H groups, UV irradiation was applied, and the C=O functionality of the benzophenone derivative reacted with the polymers' C-H bonds (insertion mechanism).…”

Section: Fully Hydrolyzed Poly(2-oxazoline)smentioning

confidence: 99%

Design Strategies for Functionalized Poly(2-oxazoline)s and Derived Materials

2013

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Abstract:The polymer class of poly(2-oxazoline)s currently is under intensive investigation due to the versatile properties that can be tailor-made by the variation and manipulation of the functional groups they bear. In particular their utilization in the biomedic(in)al field is the subject of numerous studies. Given the mechanism of the cationic ring-opening polymerization, a plethora of synthetic strategies exists for the preparation of poly(2-oxazoline)s with dedicated functionality patterns, comprising among others the functionalization by telechelic end-groups, the incorporation of substituted monomers into (co)poly(2-oxazoline)s, and polymeranalogous reactions. This review summarizes the current state-of-the-art of poly(2-oxazoline) preparation and showcases prominent examples of poly(2-oxazoline)-based materials, which are retraced to the desktop-planned synthetic strategy and the variability of their properties for dedicated applications.

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“…The polymer is based on a partially hydrolyzed poly(ethyloxazoline) to which photoreactive benzophenone units are coupled by means of a polymer analogous reaction (Chang et al 2002). The coating is then UV cured at 365 nm, 3 mW cm −2 for 1 h, yielding both crosslinking and chemical attachment to Fig.…”

Section: Disk Fabricationmentioning

confidence: 99%

Single-step centrifugal hematocrit determination on a 10-$ processing device

Riegger

Grumann

Steigert

et al. 2007

Biomed Microdevices

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We present a novel concept to process human blood on a spinning polymer disk for the determination of the hematocrit level by simple visual inspection. The microfluidic disk which is spun by a macroscopic drive unit features an upstream metering structure and a downstream blind channel where the centrifugally enforced sedimentation of the blood is performed. The bubble-free priming of the blind channel is governed by centrifugally assisted capillary filling along the sloped hydrophilic side-wall and the lid as well as the special shape of the dead end of the two-layer channel. The hematocrit is indicated at the sharp phase boundary between the plasma and the segregated cellular pellet on a disk-imprinted calibrated scale. This way, we conduct the hematocrit determination of human blood within 5 min at a high degree of linearity (R(2) = 0.999) and at a high accuracy (CV = 4.7%) spanning over the physiological to pathological working range. As all processing steps including the priming, the metering to a defined volume as well as the centrifugation are executed automatically during rotation, the concept is successfully demonstrated in a conventional PC-CDROM drive while delivering the same performance (R(2) = 0.999, CV = 4.3%).

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Surface-attached polymer monolayers for the control of endothelial cell adhesion

Cited by 40 publications

References 19 publications

Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines

Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines

Design Strategies for Functionalized Poly(2-oxazoline)s and Derived Materials

Single-step centrifugal hematocrit determination on a 10-$ processing device

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