Surface Adhesins of Staphylococcus aureus

Clarke, Simon; Foster, Simon J.

doi:10.1016/s0065-2911(06)51004-5

Cited by 245 publications

(207 citation statements)

References 199 publications

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“…The four proteins previously shown to interact with S. aureus that we did not identify in our analysis are elastin, laminin, TNFR1, and von Willebrand factor (4,12). Potential reasons for our inability to detect these proteins include the following: (i) the proteins were not present in the airway at the time points that we analyzed; (ii) the shotgun proteomics approach is not sufficiently sensitive to detect all of the proteins present in a complex mixture, particularly those present in low abundance; (iii) the proteins were present but did not bind to S. aureus in the airway; and (iv) the fractionation techniques (1D SDS-PAGE, SCX, and LC) may have eliminated proteins with extreme charges or molecular weights.…”

Section: Discussionmentioning

confidence: 63%

Host Airway Proteins Interact withStaphylococcus aureusduring Early Pneumonia

et al. 2008

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Staphylococcus aureus is a major cause of hospital-acquired pneumonia and is emerging as an important etiological agent of community-acquired pneumonia. Little is known about the specific host-pathogen interactions that occur when S. aureus first enters the airway. A shotgun proteomics approach was utilized to identify the airway proteins associated with S. aureus during the first 6 h of infection. Host proteins eluted from bacteria recovered from the airways of mice 30 min or 6 h following intranasal inoculation under anesthesia were subjected to liquid chromatography and tandem mass spectrometry. A total of 513 host proteins were associated with S. aureus 30 min and/or 6 h postinoculation. A majority of the identified proteins were host cytosolic proteins, suggesting that S. aureus was rapidly internalized by phagocytes in the airway and that significant host cell lysis occurred during early infection. In addition, extracellular matrix and secreted proteins, including fibronectin, antimicrobial peptides, and complement components, were associated with S. aureus at both time points. The interaction of 12 host proteins shown to bind to S. aureus in vitro was demonstrated in vivo for the first time. The association of hemoglobin, which is thought to be the primary staphylococcal iron source during infection, with S. aureus in the airway was validated by immunoblotting. Thus, we used our recently developed S. aureus pneumonia model and shotgun proteomics to validate previous in vitro findings and to identify nearly 500 other proteins that interact with S. aureus in vivo. The data presented here provide novel insights into the host-pathogen interactions that occur when S. aureus enters the airway.

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Section: Discussionmentioning

confidence: 63%

Host Airway Proteins Interact withStaphylococcus aureusduring Early Pneumonia

et al. 2008

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“…Twenty-one genes encoding LPXTG-proteins have been identified by in silico analysis of S. aureus genomes (13). Experimental deletion or heterologous expression of these proteins helped identify their physiological functions and infer their roles in diseases (14,15). However, although highlighting the multiple facets of S. aureus pathogenesis, none of these experiments provided a comprehensive view of the infection process.…”

mentioning

confidence: 99%

Proteomic and Transcriptomic Profiling of Staphylococcus aureus Surface LPXTG-proteins: Correlation with agr Genotypes and Adherence Phenotypes

Ythier

Resch

Waridel

et al. 2012

Molecular & Cellular Proteomics

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“…Conversely, expression of the putative B-SAg determinant by Efb may help the bacterium evade adaptive humoral immunity. S. aureus produces other virulence factors that facilitate bacterial adhesion to the extracellular matrix and host cell surface, exert direct toxic effects on cells, and subvert host immunity (71)(72)(73). One such factor, Protein A, expresses a well defined B-SAg determinant that has served as the archetype for identifying novel microbial B-SAgs.…”

Section: Discussionmentioning

confidence: 99%

Constitutive Production of Catalytic Antibodies to a Staphylococcus aureus Virulence Factor and Effect of Infection

Brown

Nishiyama

Dunkle

et al. 2012

Journal of Biological Chemistry

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Background:We examined the role of catalytic antibodies in immune defense against Staphylococcus aureus. Results: IgG from non-infected humans and mice hydrolyzed the S. aureus extracellular fibrinogen-binding protein (Efb), and the activity was reduced following infection. Conclusion: Constitutive but not adaptive production of catalytic antibodies suggests that Efb expresses B-lymphocyte superantigenic character. Significance: Efb superantigenic character may be a factor in S. aureus pathogenesis.

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Surface Adhesins of Staphylococcus aureus

Cited by 245 publications

References 199 publications

Host Airway Proteins Interact withStaphylococcus aureusduring Early Pneumonia

Host Airway Proteins Interact withStaphylococcus aureusduring Early Pneumonia

Proteomic and Transcriptomic Profiling of Staphylococcus aureus Surface LPXTG-proteins: Correlation with agr Genotypes and Adherence Phenotypes

Constitutive Production of Catalytic Antibodies to a Staphylococcus aureus Virulence Factor and Effect of Infection

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