Supranutritional dietary selenium induced hyperinsulinemia and dyslipidemia via affected expression of selenoprotein genes and insulin signal-related genes in broiler

Huang, Xiaofeng; Tang, Jieyuan; Xu, Jingyang; Jia, Gang; Liu, Guangmang; Chen, Xiaoling; Cai, Jingyi; Shang, Haiying; Zhao, Hua

doi:10.1039/c6ra14932d

Cited by 23 publications

(20 citation statements)

References 61 publications

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“…The results showed that low levels of Se supplementation in diet (0.15–0.30 ppm) could effectively enhance the mRNA level of GPX and SelW , but excessive concentrations of Se could decrease the expression (Figure e–f). These findings were in line with those expressions in rainbow trout (Wang et al, ) and chickens (Huang et al, ). Gu, Ream, and Whanger () found that Se supplementation had no effect on the transcription rate of SelW but could prolong the half‐life period and stabilize its mRNA.…”

Section: Discussionsupporting

confidence: 90%

Effect of dietary organic selenium on survival, growth, antioxidation, immunity and gene expressions of selenoproteins in abalone Haliotis discus hannai

Kong

Liu

et al. 2018

Aquac Res

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No studies have been focused on the inclusion of organic selenium in formulated feed of abalone when organic minerals are accepted by aquaculture feed industry. This experiment evaluated the effect of organic selenium on the growth, antioxidation, immunity and gene expressions of selenoproteins in abalone. Graded levels of organic selenium were included to the basal diet at 0.00, 0.15, 0.30 and 0.60 ppm providing 157.83, 322.51, 437.59 and 596.59 ug/kg of selenium in the diet. Accordingly, the diets were named as Se-0.00, Se-0.15, Se-0.30 and Se-0.60 respectively. Results had shown that survival rate, weight gain rate and shell length increase rate were lowest for Se-0.60 group (p < 0.05). The concentration of selenium in the whole body generally increased with increasing level of dietary organic selenium (p < 0.05). The enzyme activities related to antioxidation increased initially with low levels of dietary selenium and decreased with higher levels, with the highest value in Se-0.30 group (p < 0.05). The enzyme activities of immunity increased with the addition of dietary selenium. With the increase of dietary selenium, Myostain expression decreased initially and increased afterwards, with lowest value for Se-0.30 group (p < 0.05). However, the expressions of other genes related to growth, antioxidation and selenoproteins firstly increased and then decreased, with the highest value recorded for Se-0.15 group. In conclusion, the inclusion rate of 0.15 -0.30 ppm Se in diet could promote growth, antioxidation, immunity and gene expressions of selenoproteins in abalone; however, excess selenium with 0.60 ppm inclusion lever in diet had toxic effects. K E Y W O R D S abalone, antioxidation, selenium, selenoprotein, toxicity

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Section: Discussionsupporting

confidence: 90%

Effect of dietary organic selenium on survival, growth, antioxidation, immunity and gene expressions of selenoproteins in abalone Haliotis discus hannai

Kong

Liu

et al. 2018

Aquac Res

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“…Dietary Se deficiency (< 0.02 mg Se/kg) elevated fasting plasma glucose and decreased plasma insulin in birds ( Table 2 ) while it exhibited no effects on fasting plasma glucose and insulin concentration in pigs [ 10 , 35 ] and rat dams [ 9 ] models. Selenium is involved in carbohydrate and lipid metabolism in animal [ 11 , 14 ] and high Se exposure is associated with increased plasma lipid levels [ 11 ]. In present study, dietary Se deficiency induced pancreatic atrophy was associated with hypoinsulinemic hyperglycemia, and chicken also exhibited lower plasma TG and TC concentrations ( Table 2 ) .…”

Section: Discussionmentioning

confidence: 99%

“…Although its importance for the prevention of certain forms of diseases such as exudative diathesis and nutritional pancreatic atrophy in chicken [ 4 ], Keshan disease and muscular dystrophy in human [ 5 ], and muscle malnutrition in swine [ 6 ] has been well established, the association between Se and glucose metabolism remains unclear. Recent studies report that supranutritional Se intake is associated with development of insulin resistance and hyperinsulinemia, and the alteration in blood lipid profiles in human [ 7 , 8 ], rodent [ 9 ], pig [ 10 ] and chicken [ 11 ] models. However, a number of animal experiments and epidemiologic investigations have shown the correlation between Se deficiency and glucose or lipid metabolic impairment [ 4 , 12 ] and as insulin mimetic, Se can activate key proteins involved in the insulin signaling pathway and has an anti-diabetic effect [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Pancreatic atrophy caused by dietary selenium deficiency induces hypoinsulinemic hyperglycemia via global down-regulation of selenoprotein encoding genes in broilers

Wang

Tang

et al. 2017

PLoS ONE

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This study was envisaged to comprehensively profile genes in selected tissues along with a few biochemical indicators and integrate resulting information with dietary selenium (Se) deficiency symptoms in broilers. A total of 120 one-day-old Cobb male broilers were equally divided into two groups and fed a Se deficient corn-soybean-based basal diet supplemented with 0.3 mg/kg sodium selenite (Control, Se adequate) or without selenite (Se deficiency) for five weeks. Effects of Se deficiency on mRNA abundance of twenty-three selenoprotein encoding genes and seventeen insulin signaling related genes were studied at day 35 in pancreas, liver and muscle along with plasma biochemical constituents and enzyme activities. Compared to healthy birds in control diet, Se deficient diet induced deficiency symptoms in 90% birds and classic nutritional pancreatic atrophy, depressed growth performance of broilers, and decreased (P < 0.01 to P < 0.05) total antioxidant capacity and activities of superoxide dismutase and glutathione peroxidase in plasma and three other tissues. Se deficiency resulted in 58% higher mortality than control birds. Dietary Se deficiency down-regulated (P < 0.01–0.05) eighteen selenoprotein encoding genes in pancreas, fourteen genes in muscle and nine genes in liver, and up-regulated (P < 0.05) Txnrd1 and Selx in liver. Meanwhile, six, thirteen and five insulin signaling related genes were down-regulated (P < 0.01–0.05) in pancreas, muscle and liver, respectively, and three genes were up-regulated (P < 0.01) in liver. The decrease (P < 0.05) in levels of plasma insulin, total triglyceride and total cholesterol, and concurrent elevated (P < 0.05) levels of plasma glucose and inflammatory cytokines accompanied the global down-regulation of selenoprotein encoding- and insulin signaling related- genes in Se deficient birds. It was concluded that dietary Se deficiency induces nutritional pancreatic atrophy and metabolic disorder of glucose and lipid in broilers via down-regulation of selenoprotein encoding- and insulin signaling related- genes, indicating potential roles of these genes in metabolic regulation.

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“…Other studies using large numbers of chicks fed Se-deficient corn-soy diets did find significant decreases in growth in Sedeficient chicks supplemented with 1X to 5X the NRC chicken vitamin E requirement [60][61]; these studies reported that 7-9 selenotranscripts were significantly decreased by Se deficiency. Supernutritional Se supplementation studies in chicks fed corn-soy diets have reported that high dietary Se decreases [62] or increases growth [63] along with increasing expression of subsets of selenotranscripts. Our studies in the young chick [18] and turkey poult [7] only found significant changes in 8 and 4 selenoprotein transcripts out of 24, respectively, in the livers of birds fed Se-deficient torula yeast diets for 4 weeks.…”

Section: Vitamin Ementioning

confidence: 99%

The hepatic transcriptome of the turkey poult (Meleagris gallopavo) is minimally altered by high inorganic dietary selenium

et al. 2020

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There is interest in supplementing animals and humans with selenium (Se) above Se-adequate levels, but the only good biomarker for toxicity is tissue Se. We targeted liver because turkeys fed 5 μg Se/g have hepatic Se concentrations 6-fold above Se-adequate (0.4 μg Se/ g) levels without effects on growth or health. Our objectives were (i) to identify transcript biomarkers for high Se status, which in turn would (ii) suggest proteins and pathways used by animals to adapt to high Se. Turkey poults were fed 0, 0.025, 0.4, 0.75 and 1.0 μg Se/g diet in experiment 1, and fed 0.4, 2.0 and 5.0 μg Se/g in experiment 2, as selenite, and the full liver transcriptome determined by RNA-Seq. The major effect of Se-deficiency was to downregulate expression of a subset of selenoprotein transcripts, with little significant effect on general transcript expression. In response to high Se intake (2 and 5 μg Se/g) relative to Seadequate turkeys, there were only a limited number of significant differentially expressed transcripts, all with only relatively small fold-changes. No transcript showed a consistent pattern of altered expression in response to high Se intakes across the 1, 2 and 5 μg Se/g treatments, and there were no associated metabolic pathways and biological functions that were significant and consistently found with high Se supplementation. Gene set enrichment analysis also found no gene sets that were consistently altered by high-Se and supernutritional-Se. A comparison of differentially expressed transcript sets with high Se transcript sets identified in mice provided high Se (~3 μg Se/g) also failed to identify common differentially expressed transcript sets between these two species. Collectively, this study indicates that turkeys do not alter gene expression in the liver as a homeostatic mechanism to adapt to high Se.

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Supranutritional dietary selenium induced hyperinsulinemia and dyslipidemia via affected expression of selenoprotein genes and insulin signal-related genes in broiler

Abstract: The purpose of this study was to investigate the effects of supranutritional selenium (Se) on the mRNA expression of selenoprotein genes and insulin signal-related genes in the liver, muscle and pancreas of the broiler.

Cited by 23 publications

References 61 publications

Effect of dietary organic selenium on survival, growth, antioxidation, immunity and gene expressions of selenoproteins in abalone Haliotis discus hannai

Effect of dietary organic selenium on survival, growth, antioxidation, immunity and gene expressions of selenoproteins in abalone Haliotis discus hannai

Pancreatic atrophy caused by dietary selenium deficiency induces hypoinsulinemic hyperglycemia via global down-regulation of selenoprotein encoding genes in broilers

The hepatic transcriptome of the turkey poult (Meleagris gallopavo) is minimally altered by high inorganic dietary selenium

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