Suppressive effect of monosaccharides on ICAM-1/CD54 expression in human keratinocytes

Palácio, Sarah Brandão; Viac, J.; Vinche, A.; Huband, Janet C.; Gatto, Hugues; Schmitt, Daniel

doi:10.1007/s004030050185

Cited by 5 publications

(10 citation statements)

References 7 publications

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“…Apart from being essential structural components of plasma cell membranes, carbohydrates are of major functional importance because they are involved in a variety of basic cell functions, including cell-cell interaction, recognition, and adhesion, cell-substrate adhesion, signal transduction, receptor function and other key biochemical and immunological events (Walsh et al 1991, Buxton et al 1992, David 1993, Palacio et al 1997.…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative alterations of cell surface carbohydrate residues during epidermal morphogenesis

et al. 2005

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The purpose of this study was to investigate the carbohydrate residue composition of cell surface in the developing epidermis and to define the chronological sequence of its alterations in human fetuses from the 10th to the 20th weeks of gestation and at the 23rd week of gestation, using a panel of six biotinylated lectins: Concanavalin A, Ulex europaeus agglutinin-I, Ricinus communis agglutinin-I, Peanut agglutinin, Wheat germ agglutinin, and Dolichos biflorus agglutinin. Distinct qualitative and quantitative alterations in the expression of cell surface carbohydrate residues were found during epidermal morphogenesis prior to keratinization (10th to 20th weeks). At the 23rd week of gestation, the already keratinized fetal human epidermis revealed a pattern of cell surface glycosylation very similar to that of the adult human epidermis. Further studies are now warranted to answer the question regarding whether the glycosylation pattern in the developing human epidermis is disturbed in fetuses with genodermatoses and whether these disturbances might be important for prenatally diagnosing the latter.

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Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative alterations of cell surface carbohydrate residues during epidermal morphogenesis

et al. 2005

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“…In humans, IFN-γ, a crucial mediator of the immune system, and LPS are two potent stimulators of keratinocyte activation known to induce TNF-α production (Kupper, 1990) and ICAM-1 surface expression (Caughman et al, 1990). Interestingly, monosaccharides such as fucose and rhamnose have been reported to reduce human keratinocyte activation (Palacio et al, 1997b). In this study, the addition of monosaccharides to IFN-γ-treated keratinocytes downregulated CD54 expression at 24 and 48 h in vitro.…”

Section: Introductionmentioning

confidence: 81%

“…Fucose (at 20 mg/ml), rhamnose (at 10 mg/ml), arabinose (at 10 mg/ml) or fucose associated with rhamnose (at 20 mg/ml and 5 mg/ml, respectively) (all from Sigma) were added at the beginning of incubation time. These concentrations were chosen as they have been tested on human keratinocytes and have been shown to decrease CD54 expression on human cells for fucose and rhamnose (Palacio et al, 1997b). When tested together with fucose, rhamnose was tested at 5 mg/ml because higher concentrations were shown to damage human cells (Palacio et al, 1997b).…”

Section: Application: Modulation Of Canine Keratinocyte Activation Bymentioning

confidence: 99%

“…In this study, the addition of monosaccharides to IFN-γ-treated keratinocytes downregulated CD54 expression at 24 and 48 h in vitro. These monosaccharides have also been reported to have inhibitory effects on lymphokine activity in in vitro systems and can be effective in suppressing in vivo manifestations of cellular immunity (Palacio et al, 1997b). However, the effects of these immunomodulatory functions of monosaccharides on the skin are still not well understood and documented.…”

Section: Introductionmentioning

confidence: 98%

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Upregulation of TNF-α Production by IFN-γ and LPS in Cultured Canine Keratinocytes: Application to Monosaccharides Effects

et al. 2007

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Activated keratinocytes play a key role in the cutaneous immune system by their interactions with other cell types through the production of cytokines with both autocrine and paracrine activity. But there is little knowledge about epidermal cytokines in the dog. In this study, cultured canine keratinocytes were stimulated by human recombinant interferon gamma (IFN-gamma) and lipopolysaccharide (LPS) and cell supernatants were tested for tumour necrosis factor alpha (TNF-alpha) concentration using a cell viability assay on a murine cell line. We show that IFN-gamma in combination with LPS significantly increases TNF-alpha secretion by canine keratinocytes. The best stimulations were obtained using confluent cultures and the association of IFN-gamma (400 ng/ml) and LPS (40 microg/ml). The experimental protocol we describe represents a new method for studying keratinocyte activation and its modulation in the dog. We provide an example of application of our method: the study of the effects of different monosaccharides on canine keratinocyte activation.

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“…Οι μονό-και ολιγοσακχαριτες είναι ανοσορυθμι-στικές ουσίες που μπορούν να αναστειλλουν την έκ κριση των φλεγμονικών κυτταροκινών (όπως η TNF-a) και να περιορίσουν την έκφραση των διαμεμβρανικών μορίων (όπως τα ICAM 1), όπως διαπιστώθηκε σε με λέτες που έγιναν στον άνθρωπο 50 και το σκΰλο 51 . Η βι ταμίνη Ε είναι αντιοξειδωτική ουσία, σταθεροποιεί τα λυσοσωμάτια, περιορίζει τη σΰνθεση της προσταγλαν-δινης Ε2 (PGE2) και αυξάνει την παραγωγή της ιντερ-λευκινης 2 (IL-2), γεγονός που εξηγεί την αντιφλεγμο-νώδη και ανοσοδιεγερτική της δράση 13 .…”

Section: η χρήση των σαμπουάν στις αλλεργικές δερματίτιδεςunclassified

The art of shampoos in canine and feline dermatology: Treatment and prevention strategies

Carlotti¹,

Gatto

2017

J Hellenic Vet Med Soc

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This paper reviews the current technology pertaining to veterinary shampoos used for dogs and cats. The criteria used to evaluate their efficacy are presented. The indications for the use of shampoos in keratoseborrhoeic disorders, parasitic diseases, bacterial diseases, fungal diseases and allergic diseases are reviewed. In keratoseborrhoeic disorders both keratomodulating and antiseborrhoeic agents are used. Antiparasitic shampoos are not frequendy used in veterinary dermatology and their indications and limitations are discussed. Antibacterial, antifungal and antiseptic agents are included in shampoos designed to treat dermatoses resulting from microbiological agents. Their indications and efficacy are presented. Shampoos are used nowadays in conjunction with other forms of therapy to treat allergic skin diseases. The benefits of such adjunctive topical therapy are discussed. Lastly, the properties of moisturizers are presented and the value of their use in combination with therapeutic shampoos is emphasized.

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Suppressive effect of monosaccharides on ICAM-1/CD54 expression in human keratinocytes

Cited by 5 publications

References 7 publications

Qualitative and quantitative alterations of cell surface carbohydrate residues during epidermal morphogenesis

Qualitative and quantitative alterations of cell surface carbohydrate residues during epidermal morphogenesis

Upregulation of TNF-α Production by IFN-γ and LPS in Cultured Canine Keratinocytes: Application to Monosaccharides Effects

The art of shampoos in canine and feline dermatology: Treatment and prevention strategies

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