Suppression of adipocyte differentiation by<i>Cordyceps militaris</i>through activation of the aryl hydrocarbon receptor

Shimada, Tsuyoshi; Hiramatsu, Naoki; Kasai, Ayumi; Mukai, Mai; Okamura, Maro; Yao, Jian; Huang, Tao; Tamai, Minori; Takahashi, Shuhei; Nakamura, Tomoyuki; Kitamura, Masanori

doi:10.1152/ajpendo.90373.2008

Cited by 23 publications

(18 citation statements)

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“…For the induction of adipocyte differentiation, cells were (1) precultured in basal medium for 2 days, (2) treated with differentiation medium containing 10 mg/ml insulin, 0.25 mM dexamethasone and 500 mM IBMX (IDI medium) for 2 days (D2 adipocyte) and (3) incubated in basal medium supplemented with insulin alone for 2 days (D4 adipocyte), as we have described previously. 7 The cells were further incubated in basal medium for additional 2 days (D6 adipocyte) and subjected to analyses.…”

Section: Induction Of Adipocyte Differentiationmentioning

confidence: 99%

“…After the staining, cells were rinsed several times with 60% isopropanol and subjected to microscopic analysis. 7 To evaluate the amount of lipid quantitatively, we added cells with isopropanol containing 4% Nonidet P-40, lysed by agitation for 5 min and subjected to spectrophotometric analysis (absorbance: 520 nm wavelength).…”

Section: Oil Red O Stainingmentioning

confidence: 99%

“…7,9 cDNAs for adiponectin, 10 PPARg (purchased from Addgene, Cambridge, MA, USA), 11 C/EBPa, C/EBPb and C/EBPd 12 (provided by Dr Ez-Zoubir Amri, CNRS, Nice, France) were used for preparation of radiolabeled probes. The level of 28S rRNA was used as a loading control.…”

Section: Northern Blot Analysismentioning

confidence: 99%

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Selective deletion of adipocytes, but not preadipocytes, by TNF-α through C/EBP- and PPARγ-mediated suppression of NF-κB

Tamai

Shimada

Hiramatsu

et al. 2010

Laboratory Investigation

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Tumor necrosis factor-a (TNF-a) is a key regulator of adipose tissue mass, but mechanisms underlying this effect have not been fully elucidated. We found that exposure to TNF-a caused a significant decrease in the number of adipocytes, but not preadipocytes. Subsequent experiments revealed that TNF-a selectively deleted adipocytes through induction of apoptosis. Following exposure to TNF-a, rapid activation of nuclear factor-kB (NF-kB) was observed only in preadipocytes, but not in adipocytes. Inhibition of NF-kB rendered preadipocytes susceptible to TNF-a-induced apoptosis, suggesting that different activity of NF-kB is the determinant for the distinct apoptotic responses. During adipocyte differentiation, expression and activity of peroxisome proliferator-activated receptor-g (PPARg) were upregulated. Treatment of preadipocytes with a PPARg agonist attenuated NF-kB activation and rendered the cells vulnerable to TNF-a-induced apoptosis. Conversely, treatment of adipocytes with a PPARg antagonist enhanced NF-kB activation and conferred resistance to TNF-a-induced apoptosis, suggesting involvement of PPARg in the suppression of NF-kB in adipocytes. We also found that, following differentiation, expression and activity of CCAAT/ enhancer binding protein (C/EBP), especially C/EBPa and C/EBPb, were upregulated in adipocytes. Overexpression of individual C/EBPs significantly inhibited activation of NF-kB in preadipocytes. Furthermore, transfection with siRNA for C/EBPa or C/EBPb enhanced activity of NF-kB in adipocytes, suggesting that C/EBP is also involved in the repression of NF-kB in adipocytes. These results suggested novel mechanisms by which TNF-a selectively deletes adipocytes in the adipose tissue. The C/EBP-and PPARg-mediated suppression of NF-kB may contribute to TNF-a-related loss of adipose tissue mass under certain pathological situations, such as cachexia.

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Section: Induction Of Adipocyte Differentiationmentioning

confidence: 99%

Section: Oil Red O Stainingmentioning

confidence: 99%

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Selective deletion of adipocytes, but not preadipocytes, by TNF-α through C/EBP- and PPARγ-mediated suppression of NF-κB

Tamai

Shimada

Hiramatsu

et al. 2010

Laboratory Investigation

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“…Thus, PUFA plays a central role in suppressing fatty acid synthesis and regulating adipocyte differentiation through suppression of late phase adipocyte differentiation [233,234]. Recent reports have demonstrated another interesting mechanism, in the extract of macro fungus Cordyceps militaris mycelia, which suppressed 3T3-L1 adipocyte differentiation through activation of the aryl hydrocarbon receptor [235]. The wide variety of natural products found to inhibit preadipocyte proliferation and/or the apoptotic effect.…”

Section: Inhibitory Effect On Adipocyte Differentiationmentioning

confidence: 99%

Obesity: Development, Epidemiology, Factors Affecting, Quantity, Health Hazards, Management and Natural Treatment-a Review

Bollapragada

Shantaram

Kumar

2017

Int J Pharm Pharm Sci

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Due to the advancement in science, enhanced knowledge on the physiological aspects of almost all the tissues and the organs of the human body is gained. One of the most important prevalent topics needed for discussion is obesity and its effect on the metabolic changes leading to disorders in the human body such as diabetes, hypertension, cardiovascular diseases in addition to chronic diseases such as stroke, osteoarthritis, sleep apnea, some cancers, and inflammation-based pathologies. In recent years, obesity is a serious socioeconomic issue, which has become one of the major health problems all over the world, affecting people of all ages, sex, ethnicities and races. Obesity is a complex and multifactorial disease caused by the interaction of a myriad genetics, dietary, lifestyle and environmental factors and it is characterised by an excessive weight for height due to an enlarged fat deposition in the adipose tissue, which is due to a higher calorie intake than the energy expenditure. The pharmaceutical drugs are currently available to treat obesity but generally they have unpleasant side effects. Recent researches demonstrated the potential of natural products to counteract on obesity. Now the novel promising approach is the usage of dietary supplements and plant products and their bioactive compounds that could interfere on pancreatic lipase activity, food intake, lipid metabolism and adipocyte differentiation. In a similar way, hundreds of extracts are currently being isolated from plants, fungi, algae or bacteria and are screened for their potential inhibitions of activity against obesity. Natural products may have a synergistic activity that increases their bioavailability and action on multiple molecular targets.

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“…For example, Undaria pinnatifida (brown algae) [23], garlic [1], and Humulus lupulus (Common hop) [25] showed inhibition of 3T3-L1 preadipiocyte differentiation. Meanwhile, Garcina cambogia [13] and Cordyceps militaris [32] exhibited decreased accumulation of lipids or triglycerides (TG) in 3T3-L1 preadipocytes.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory activity of Phellodendri cortex extracts on differentiation of 3T3-L1 preadipocytes

Choi

Yun

2011

Korean J. Chem. Eng.

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The purpose of the present study was to investigate the inhibitory effect of beberine-rich fraction of Phellodendri cortex extract (PC) on adipogenesis in 3T3-L1 cells. PC effectively prevented TG accumulation in differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. Compared to controls, PC at a concentration of 75 µg/mL significantly decreased lipid droplets by 79.5%. Beberine exhibited similar inhibitory effect to that of PC extract, suggesting that fractionated PC extract contained mostly berberine. The expression levels of PAARγ and C/EBPα were significantly reduced by PC treatment. This result consequently led to suppression of expression of LPL, FABP4, CD36, and SCD2, which are target genes of PPARγ and C/EBPα. PC also inhibited protein levels of PPARγ, FABP4, and GLUT4. Accordingly, these results suggest that PC inhibited adipogenesis in 3T3-L1 preadipocyte cells through PPARγ and CEBPα-related pathways at both transcript and protein levels.

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Suppression of adipocyte differentiation byCordyceps militaristhrough activation of the aryl hydrocarbon receptor

Cited by 23 publications

References 50 publications

Selective deletion of adipocytes, but not preadipocytes, by TNF-α through C/EBP- and PPARγ-mediated suppression of NF-κB

Selective deletion of adipocytes, but not preadipocytes, by TNF-α through C/EBP- and PPARγ-mediated suppression of NF-κB

Obesity: Development, Epidemiology, Factors Affecting, Quantity, Health Hazards, Management and Natural Treatment-a Review

Inhibitory activity of Phellodendri cortex extracts on differentiation of 3T3-L1 preadipocytes

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