Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos

Botigelli, Ramon César; Razza, E. M.; Pioltine, Elisa Mariano; Fontes, P. K.; Schwarz, Kátia Regina Lancellotti; Leal, Cláudia Lima Verde; Nogueira, Marcelo Fábio Gouveia

doi:10.1016/j.repbio.2018.01.004

Cited by 16 publications

(13 citation statements)

References 51 publications

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“…Embryos were produced in vitro from cumulus-oocytes complex from antral follicles of slaughtered cows based on the protocol previously published [50]. On the day 7 of in vitro culture (day 0 was on in vitro insemination), the embryos were recovered from the culture.…”

Section: Methodsmentioning

confidence: 99%

Distinct Sources of a Bovine Blastocyst Digital Image do not Produce the Same Classification by a Previously Trained Software using Artificial Neural Network

Guilherme

Pronunciate

Santos

et al. 2018

Preprint

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We develop an online graphical and intuitive interface connected to a server aiming to facilitate access to professionals worldwide that face problems with bovine blastocysts classification. The interface Blasto3Q (3Q is referred to the three qualities of the blastocyst grading) contains a description of 24 variables that are extracted from the image of the blastocyst and analyzed by three Artificial Neural Networks (ANNs) that classifies the same loaded image. The same embryo (i.e., the biological specimen) was submitted to digital image capture by the control group (inverted microscope with 40x of magnification) and to experimental group (stereomicroscope with maximum of magnification plus 4x zoom from the cell phone).The 36 images obtained from control and experimental groups were uploaded on the Blasto3Q. Each image from both sources was evaluated for segmentation and submitted (only if it could be properly or partially segmented) to the quality grade classification by the three ANNs of the Blasto3Q program. In the group control, all the images were properly segmented, whereas 38.9% (07/18) and 61.1% (11/18) of the images from the experimental group, respectively could not be segmented or were partially segmented. The percentage of agreement was calculated when the same blastocyst was evaluated by the same ANN from the two sources (control and experimental groups). On the 54 potential evaluations of the three ANNs (i.e., 18 images been evaluated by the three networks) from the experimental group only 22.2% agreed with evaluations of the control (12/54). Of the remaining 42 disagreed evaluations from experimental group, 21 were unable to be performed and 21 were wrongly processed when compared with control evaluation.

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Section: Methodsmentioning

confidence: 99%

Distinct Sources of a Bovine Blastocyst Digital Image do not Produce the Same Classification by a Previously Trained Software using Artificial Neural Network

Guilherme

Pronunciate

Santos

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Embryos were produced in vitro from the cumulus-oocytes complex from the antral follicles of slaughtered cows based on the protocol previously published [51]. On days 7 to 8.5 of in vitro culture (day 0 was in vitro insemination), the embryos were recovered from the culture.…”

Section: Methodsmentioning

confidence: 99%

Artificial Intelligence-Based Grading Quality of Bovine Blastocyst Digital Images: Direct Capture with Juxtaposed Lenses of Smartphone Camera and Stereomicroscope Ocular Lens

Nogueira

Guilherme

Pronunciate

et al. 2018

Sensors

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In this study, we developed an online graphical and intuitive interface connected to a server aiming to facilitate professional access worldwide to those facing problems with bovine blastocysts classification. The interface Blasto3Q, where 3Q refers to the three qualities of the blastocyst grading, contains a description of 24 variables that were extracted from the image of the blastocyst and analyzed by three Artificial Neural Networks (ANNs) that classify the same loaded image. The same embryo (i.e., the biological specimen) was submitted to digital image capture by the control group (inverted microscope with 40× magnification) and the experimental group (stereomicroscope with maximum of magnification plus 4× zoom from the cell phone camera). The images obtained from the control and experimental groups were uploaded on Blasto3Q. Each image from both sources was evaluated for segmentation and submitted (only if it could be properly or partially segmented) for automatic quality grade classification by the three ANNs of the Blasto3Q program. Adjustments on the software program through the use of scaling algorithm software were performed to ensure the proper search and segmentation of the embryo in the raw images when they were captured by the smartphone, since this source produced small embryo images compared with those from the inverted microscope. With this new program, 77.8% of the images from smartphones were successfully segmented and from those, 85.7% were evaluated by the Blasto3Q in agreement with the control group.

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“…Some studies have shown that manipulating the level of cGMP with CNP in the maturation medium decreases the lipid content in the oocyte 22 . Lipolysis results from the sequential activity of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and mono-acyl glycerol lipase (MAG) 23 .…”

Section: Introductionmentioning

confidence: 99%

“…Activation of ATGL plays key role in initiation of lipolysis 24 . Also, perilipin (PLIN2) and diacylglycerol acyltransferase 1 (DGAT1) are required for the accumulation and stability of the LDs 22 . Higher cytoplasmic lipid content observed in in vitro produced (IVP) embryos in compare to their in vivo counterparts is one of the factors responsible for their lower cryosurvival rate 25 , 26 .…”

Section: Introductionmentioning

confidence: 99%

“…Higher cytoplasmic lipid content observed in in vitro produced (IVP) embryos in compare to their in vivo counterparts is one of the factors responsible for their lower cryosurvival rate 25 , 26 . Lower lipolysis rate is one of the shortcomings of in vitro matured oocytes as compare with in vivo matured ones, which can be remedied with some metabolites such as forskolin, L-carnitine, and CNP 22 , 27 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sequential IVM by CNP preincubation and cooperating of PGE2 with AREG enhances developmental competence of SCNT reconstructs in goat

Assareh

Shahemabadi

Varnosfaderani

et al. 2022

Sci Rep

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Developmental competence of in vitro matured cumulus oocyte complexes (COCs) in conventional IVM (C.IVM) is lower than in vivo maturated COCs and is related to unsynchronized nuclear and cytoplasmic maturation. To overcome this dearth, COCs can be exposed to granulosa secreted factors in a two-step system. Therefore, in the first experiment, 1000 nM of C-type natriuretic peptide for 8 h was determined (CAPA), as the best time and concentration to retain oocytes in germinal vesicle stage. This condition, also reduces lipid droplets and increases the expression of ATGL and PLIN2 involved in lipolysis and lipogenesis, respectively. In the second experiment, maturation was stimulated with prostaglandin E2 and amphiregulin for 18 h (CAPA-IVM), and their optimal concentrations based on blastocyst formation rates through in vitro fertilization (IVF) were determined as 1 and 600 nM, respectively. In the third experiment, the in vitro and in vivo developmental competency of SCNT embryos in CAPA-IVM group were determined. Despite similar blastocyst formation rates in IVF and SCNT between CAPA-IVM and C.IVM, the quality of blastocysts were quality was higher in CAPA-IVM, which reflected itself, as higher ICM/TE ratio and also expression of NANOG in SCNT blastocysts. Pregnancy rate, live births rate and SCNT efficiency were not significant between CAPA-IVM and C.IVM groups. Therefore, CAPA-IVM can improve the developmental competency of SCNT derived embryos.

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Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos

Cited by 16 publications

References 51 publications

Distinct Sources of a Bovine Blastocyst Digital Image do not Produce the Same Classification by a Previously Trained Software using Artificial Neural Network

Distinct Sources of a Bovine Blastocyst Digital Image do not Produce the Same Classification by a Previously Trained Software using Artificial Neural Network

Artificial Intelligence-Based Grading Quality of Bovine Blastocyst Digital Images: Direct Capture with Juxtaposed Lenses of Smartphone Camera and Stereomicroscope Ocular Lens

Sequential IVM by CNP preincubation and cooperating of PGE2 with AREG enhances developmental competence of SCNT reconstructs in goat

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