Supplemented base medium containing Amburana cearensis associated with FSH improves in vitro development of isolated goat preantral follicles

Growth differentiation factor‐9 improves development, mitochondrial activity and meiotic resumption of sheep oocytes after in vitro culture of secondary follicles

“…Similar results were reported after culture of goat secondary follicles in medium containing 100 or 200 ng/ml GDF‐9 associated with FSH (Almeida et al, ). Although FSH is important for follicle survival and growth (Barros et al, ; Gouveia et al, ), when both GDF‐9 and FSH were added to the culture medium, the positive effects of GDF‐9 alone were not recorded (Almeida et al, ). Therefore, our results could be justified by the fact that FSH may induce the expression of intra‐ovarian factors inhibiting any additional effect of GDF‐9.…”

“…The in vitro culture of isolated secondary follicles from small ruminants is an important strategy to evaluate the quality of gametes after cryopreservation (Lunardi et al, , ) or after the exposure to plant extracts (Cavalcante et al, ; Gouveia et al, ) or to metabolic stressors (Nandi, Tripathi, Gupta, & Mondal, ), as well as to increase the production of meiotically competent oocytes (Silva et al, ) or embryos (Arunakumari, Shanmugasundaram, & Rao, ; Luz et al, ). However, in sheep, the current culture system still has low efficiency with a small number of mature oocytes and embryos produced.…”

Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

“…Antioxidant compounds have been used to minimize oxidative stress and to promote growth after in vitro culture of ovarian follicles (Lins et al, ; Silva et al, ), including medicinal plant extracts (Barberino et al, ; Gouveia et al, ). However, in this study, no significant results were found in the ROS formation.…”

Supplemented Morus nigra extract‐based medium associated with FSH enables the survival and growth of isolated ovine secondary ovarian follicles

“…This hypothesis was confirmed because supplemented α‐MEM presented more morphologically normal follicles (93.1%) than non‐supplemented (72.4%) and supplemented (67.9%) M. nigra extract at the end of the culture. Although the α‐MEM contains non‐essential amino acids, carbohydrates, sodium pyruvate, lipoic acid, biotin, vitamins and DNA precursors (Faustino et al., ), preantral follicle development require factors beyond the standard culture media additives, including insulin, transferrin, selenium, glutamine, hypoxanthine, BSA and ascorbic acid (Abedelahi, Salehnia, Allameh, & Davoodi, ; Gouveia et al., ; Rodrigues et al., ; Silva et al., ). However, addition of FSH to supplemented α‐MEM did not result in any additive effect in the in vitro follicular viability and development, which was similar to that observed in α‐MEM + .…”

“…α‐MEM associated with commercial supplements) could be expensive and less accessible. Therefore, the use of alternative media composed of medicinal plant extracts may be a promising and low‐cost strategy for the in vitro culture of ovarian follicles (Gouveia et al., ), especially due to the remarkable cytoprotective and antioxidant characteristics of those plants (Tag, ).…”