Supplementation of freezing and thawing media with brain-derived neurotrophic factor protects human sperm from freeze-thaw-induced damage

Najafi, Ali; Asadi, Ebrahim; Moawad, Adel R.; Mikaeili, Saideh; Amidi, Fardin; Adutwum, Emmanuel; Safa, Majid; Sobhani, Aligholi

doi:10.1016/j.fertnstert.2016.09.004

Cited by 29 publications

(14 citation statements)

References 65 publications

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“…Finally, 54 articles were selected for assessment. During full‐text screening, no common antioxidants were identified in nineteen articles (Asadmobini, Bakhtiari, Khaleghi, Esmaeili, & Mostafaei, ; Brugnon et al, ; Fontoura et al, ; Isachenko et al, ; Kotdawala et al, ; Lu et al, ; Meamar et al, ; Naeini, Bafrani, & Nikzad, ; Najafi et al, ; Oehninger, Duru, Srisombut, & Morshedi, ; Parameswari et al, ; Pariz & Hallak, ; Saeednia et al, ; Sbracia, Grasso, Sayme, Stronk, & Huszar, ; Sobhani, Eftekhaari, Shahrzad, Natami, & Fallahi, ; Taylor, Roberts, Sanders, & Burton, ; Vickram et al, ; Werner et al, ), twelve articles lacked common sperm parameters (Banihani, Agarwal, Sharma, & Bayachou, ; Branco, Garcez, Pasqualotto, Erdtman, & Salvador, ; Donnelly, McClure, & Lewis, ; Esteves, Spaine, & Cedenho, ; Gadea et al, ; Garcez et al, ; Garcez, Branco, Lara, Pasqualotto, & Salvador, ; Jenkins, Aston, & Carrell, ; Li, Lin, Liu, Xiao, & Liu, ; Minaei et al, ; Nabi et al, ; Shabani Nashtaei et al, ) and one article lacked sufficient data (Karimfar et al, ). Finally, 23 articles (Aliabadi, Jahanshahi, Talaei‐Khozani, & Banaei, ; Askari, Check, Peymer, & Bollendorf, ; Azadi, Tavalaee, Deemeh, Arbabian, & Nasr‐Esfahani, ; Bateni et al, ; Deng et al, ; Duru, Morshedi, Schuffner, & Oehninger, ; Esteves, Sharma, Thomas, & Agarwal, ; Ghorbani et al, ; Kalthur et al, ; Keshtgar, Iravanpour, Gharesi‐Fard, & Kazerooni, ; Martinez‐Soto, Dioshourcade, Gutiérrez‐Adán, Landeras, & Gadea, ; Merino et al, ; Moubasher, Din, Ali, El‐sherif, & Gaber, ; Najafi et al, ; Nekoonam, Nashtaei, Naji, Zangi, & Amidi, ; Rossi, Mazzilli, Delfino, & Dondero, ; Taylor et al, ; Thomson et al, ; Varghese et al, …”

Section: Resultsmentioning

confidence: 99%

“…Finally, 54 articles were selected for assessment. During full-text screening, no common antioxidants were identified in nineteen articles (Asadmobini, Bakhtiari, Khaleghi, Esmaeili, & Mostafaei, 2017;Brugnon et al, 2013;Fontoura et al, 2017;Isachenko et al, 2008;Kotdawala et al, 2012;Lu et al, 2018;Meamar et al, 2012;Naeini, Bafrani, & Nikzad, 2014;Najafi et al, 2016…”

Section: Study Selection Processmentioning

confidence: 99%

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The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

et al. 2020

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To investigate the impact of antioxidants in sperm parameters and reduction in reactive oxygen species production during the freeze‐thaw process. PubMed, Scopus, Web of Science, Embase and Cochrane central library were systematically searched. Of the 1583 articles, 23 studies were selected for data extraction. Our results show that antioxidants improved sperm progressive motility (standardised mean difference (SMD) = 1; 95% CI: 0.62, 1.38; p < .001) and viability (SMD = 1.20; 95% CI: 0.50, 1.91; p = .001) and reduced sperm DNA fragmentation (SDF) and hydrogen peroxide (H2O2) production, but there was no significant improvement in total sperm motility after thawing. Acetyl‐l‐carnitine/l‐carnitine, melatonin and catalase had a significant positive impact on progressive motility. The role of tempol and melatonin in improving viability was significant compared to other antioxidants. Moreover, a significant reduction in SDF was observed after addition of butylated hydroxytoluene, tempol and vitamin E. However, the prevention of H2O2 production was significant only after the addition of tempol. Our overall results displayed the positive impact of antioxidants on progressive sperm motility, viability and reduction in SDF and H2O2 production, but no significant impact of antioxidants on total sperm motility was seen during the freeze‐thaw process.

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Section: Resultsmentioning

confidence: 99%

Section: Study Selection Processmentioning

confidence: 99%

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

et al. 2020

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“…The PPAR γ agonist rosiglitazone induced clear improvements in mitochondrial function and reduced caspase 3 activity and these effects were also linked to increased phosphorylation of Akt. Previous reports indicate the importance of Akt phosphorylation in sperm function [18, 19, 82, 83] and recently a link between PPARγ agonists and Akt phosphorylation in human [28] and pig spermatozoa [29] has been reported. Moreover, strategies to maintain Akt phosphorylation in spermatozoa have proven to be successful in human sperm cryopreservation [82, 84].…”

Section: Discussionmentioning

confidence: 99%

Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through regulating Akt phosphorylation and reduction of caspase 3

et al. 2019

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Background The population of stallion spermatozoa that survive thawing experience compromised mitochondrial functionality and accelerated senescence, among other changes. It is known that stallion spermatozoa show very active oxidative phosphorylation that may accelerate sperm senescence through increased production of reactive oxygen species. Rosiglitazone has been proven to enhance the glycolytic capability of stallion spermatozoa maintained at ambient temperature. Objectives Thus, we hypothesized that thawed sperm may also benefit from rosiglitazone supplementation. Materials and methods Thawed sperm were washed and resuspended in Tyrodes media, and the samples were divided and supplemented with 0 or 75 μM rosiglitazone. After one and two hours of incubation, mitochondrial functionality, Akt phosphorylation and caspase 3 activity were evaluated. Additional samples were incubated in the presence of an Akt1/2 inhibitor, compound C (an AMPK inhibitor) or GW9662 (an antagonist of the PPARγ receptor). Results Rosiglitazone maintained Akt phosphorylation and reduced caspase 3 activation (p<0.01), both of which were prevented by incubation in the presence of the three inhibitors. Rosiglitazone also enhanced mitochondrial functionality (P<0.01). Conclusion We provide the first evidence that the functionality of frozen stallion spermatozoa can be potentially improved after thawing through the activation of pro survival pathways, providing new clues for improving current sperm biotechnology.

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“…The PPAR γ agonist rosiglitazone induced clear improvements in mitochondrial function, and reduced caspase 3 activity, these effects also linked to increased phosphorylation of Akt. Previous reports indicate the importance of Akt phosphorylation in sperm function [15, 16, 52, 53], and recently a link between PPARγ agonists and Akt phosphorylation in human [22] and pig spermatozoa [23] has been reported. Moreover strategies to maintain Akt phosphorylated in spermatozoa have proven to be successful in human sperm cryopreservation [52, 54]…”

Section: Discussionmentioning

confidence: 99%

Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through Akt phosphorylation and reduction of caspase 3

Ortiz-Rodríguez

Silva

Masot

et al. 2019

Preprint

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20 Background 21 The population of stallion spermatozoa surviving thawing, experience, among other changes, 22 compromised mitochondrial functionality and accelerated senescence. It is known that the 23 stallion spermatozoa show very active oxidative phosphorylation that may accelerate sperm 24 senescence through increased production of reactive oxygen species. Rosiglitazone has proven 25 to enhance the glycolytic capability of stallion spermatozoa maintained in refrigeration. Objectives27 Thus, we hypothesized that thawed sperm may also benefit from rosiglitazone supplementation. Material and Methods29 Thawed sperm doses were washed and re-suspended in Tyrodes media, split sampled and 30 supplemented with 0 or 75 M rosiglitazone. After 1 and two hours of incubation, 31 mitochondrial functionality, Akt phosphorylation and caspase 3 activity were evaluated. Further 32 samples were incubated in presence of the Akt1/2 inhibitor, compound C (AMPK inhibitor) and 33 GW9662 (antagonist of the PPAR receptor). Results35 Rosiglitazone maintained Akt phosphorylated and reduced caspase 3 activation (p<0.01) that 36 was prevented by incubation in presence of the three inhibitors. Rosiglitazone also enhanced 37 mitochondrial functionality (p<0.01). Conclusion39 We provide, for the first time, evidences that the functionality of frozen stallion spermatozoa 40 can be potentially improved after thawing through the activation pro survival pathways, opening 41 new clues to improve current sperm biotechnologies. 42 43 44 45 INTRODUCTION 46 47The stallion spermatozoa can be stored in the liquid state for short periods, or frozen for long-48 term storage. Frozen thawed sperm has numerous advantages, however its wider use is still 49 constrained by a number of weaknesses [1]. Among them, the high stallion-to-stallion 50 variability and the insufficient standardization of the freezing and thawing procedures. While 51 cryopreservation induces mortality to on average 50% of the initial sperm population, the 52 surviving spermatozoa are not completely functional; on the contrary they experience 53 accelerated senescence that requires more intense and costly mare management for insemination 54 to compensate this reduced lifespan. Most of the studies on sperm cryopreservation have aimed 55 to increase the number of spermatozoa surviving the procedure, but studies aiming to improve 56 the quality of the surviving population are scarce. Although the changes induced by 57 cryopreservation have been extensively investigated, mostly focusing on cryopreservation 58 induced necrosis [2][3][4], few studies have addressed the physiology of spermatozoa surviving 59 freezing and thawing. There are not many studies that have tried to develop measures to 60 increase the quality of frozen sperm after thawing, with the exception of procedures to remove 61 dead and damaged spermatozoa from the cryopreserved sample [5][6][7]. The population of 62 spermatozoa surviving freezing and thawing, experience changes recently termed spermptosis 63 [8]. These change...

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Supplementation of freezing and thawing media with brain-derived neurotrophic factor protects human sperm from freeze-thaw-induced damage

Cited by 29 publications

References 65 publications

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through regulating Akt phosphorylation and reduction of caspase 3

Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through Akt phosphorylation and reduction of caspase 3

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