Supplementary ultraviolet‐B irradiation reveals differences in stress responses between <i>Arabidopsis thaliana</i> ecotypes*

Kalbina, Irina; Strid, Åke

doi:10.1111/j.1365-3040.2005.01436.x

Cited by 65 publications

(40 citation statements)

References 50 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The intraecotypic differences reflect natural variation that is fixed in discrete genotypes because of the self-fertilizing nature of Arabidopsis (Breyne et al, 1999). Wassilewskija has previously been shown to contain altered red/ far-red responses and respond differently to stress than Columbia (Aukerman et al, 1997;Kalbina and Strid, 2006). This work highlights another difference as described by AS rates.…”

Section: Divergence In As Rates Within Speciesmentioning

confidence: 71%

Comparative Cross-Species Alternative Splicing in Plants

et al. 2007

View full text Add to dashboard Cite

Alternative splicing (AS) can add significantly to genome complexity. Plants are thought to exhibit less AS than animals. An algorithm, based on expressed sequence tag (EST) pairs gapped alignment, was developed that takes advantage of the relatively small intron and exon size in plants and directly compares pairs of ESTs to search for AS. EST pairs gapped alignment was first evaluated in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and tomato (Solanum lycopersicum) for which annotated genome sequence is available and was shown to accurately predict splicing events. The method was then applied to 11 plant species that include 17 cultivars for which enough ESTs are available. The results show a large, 3.7-fold difference in AS rates between plant species with Arabidopsis and rice in the lower range and lettuce (Lactuca sativa) and sorghum (Sorghum bicolor) in the upper range. Hence, compared to higher animals, plants show a much greater degree of variety in their AS rates and in some plant species the rates of animal and plant AS are comparable although the distribution of AS types may differ. In eudicots but not monocots, a correlation between genome size and AS rates was detected, implying that in eudicots the mechanisms that lead to larger genomes are a driving force for the evolution of AS.Alternative RNA processing pathways result in the combining of different splice junctions that are present in pre-mRNA transcripts. In this way, a genetic unit can have a variety of mRNA and protein products, thus expanding the potential informational content of eukaryotic genomes. Recent evidence indicates a high incidence (up to 60%) of alternative splicing (AS) is present in the human genome, predominantly in the form of exon skip while a minor form is of the type called intron retention (5%-16%; Kan et al., 2002;Modrek and Lee, 2002;Carninci et al., 2005;Nagasaki et al., 2005). Although rare, intron retention can play an important biological role. It has been linked to tumor growth and is part of developmental regulation of proinsulin expression (Mansilla et al., 2005).Plants are thought to exhibit less AS and, unexpectedly, analysis in Arabidopsis (Arabidopsis thaliana) showed that intron retention is the most common type of AS, comprising 45% of the AS types (Iida et al., 2004;Ner-Gaon et al., 2004;Nagasaki et al., 2005;Wang and Brendel, 2006). The plants that were used, Arabidopsis and rice (Oryza sativa), showed comparable results. Furthermore, most AS clusters consist of two isoforms (Campbell et al., 2006). Indeed, plant intron sequence and control of splicing differ from their vertebrate and yeast (Saccharomyces cerevisiae) counterparts (Brown et al., 2002). Plant retained introns were shown to be present in RNA derived from polyribosomes, demonstrating that these intron retention events are not the by-product of incomplete splicing but are found in a potentially translatable context in the cytoplasm (NerGaon et al., 2004). Using a different method, Iida et al. (2004) aligned RIKEN Arabidopsis full-le...

show abstract

Section: Divergence In As Rates Within Speciesmentioning

confidence: 71%

Comparative Cross-Species Alternative Splicing in Plants

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The minor diVerences in PSI polypeptide composition observed between psae1-3 and double mutant plants, in fact, would support this hypothesis. Alternatively, the diVerent stability of PSI in psae1-1 and -2 on one side, and psae1-3 on the other, could also be related to ecotype-speciWc diVerences in photosensitivity: both psae1-1 and -2 are in the Col-0 ecotype background (Varotto et al 2000), whereas psae1-3 is in the Ws background known to be more susceptible than other ecotypes to reactive oxygen species-induced damages (Kalbina and Strid 2006;Li et al 2006). Thus, the reduced amount of the E subunit could be more harmful to psae1-3 than to psae1-1 and -2 leaves.…”

Section: Discussionmentioning

confidence: 99%

The E subunit of photosystem I is not essential for linear electron flow and photoautotrophic growth in Arabidopsis thaliana

Ihnatowicz¹,

Pesaresi

Leister

2007

Planta

View full text Add to dashboard Cite

PSI-E is part of the stromal side of photosystem I (PSI). In Arabidopsis thaliana, the two nuclear genes PsaE1 and PsaE2 code for PSI-E, and transcripts of PsaE1 are markedly more abundant than PsaE2 transcripts. Stable null alleles of the two PsaE genes, psae1-3 and psae2-1, were identified and characterised. The psae2-1 mutant exhibited wild-type like PSI-E abundance and photosynthetic performance, whereas in the psae1-3 mutant PSI-E accumulation was decreased by 85%, together with an impaired thylakoid electron flow and plant growth rate. The psae1-3 psae2-1 double mutant totally lacked PSI-E but was still able to grow photoautotrophically, implying that PSI-E is not essential for PSI accumulation and thylakoid electron flow.

show abstract

“…The lack of consistent effects of UV-B radiation on some plant physiological characteristics may be due to differences in plant or ecotype responses (Kalbina and Strid 2006) or differences in UV-B dose or UV-B to PAR ratios (Fiscus and Booker 1995;Nogué s and Baker 1995;Allen et al 1998). Plants respond to low levels of UV-B as a signal through a UV-B specific signaling pathway (Kim et al 1998;Brown et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Response of photosynthesis to high light and drought for Arabidopsis thaliana grown under a UV-B enhanced light regime

2006

View full text Add to dashboard Cite

Arabidopsis thaliana grown in a light regime that included ultraviolet-B (UV-B) radiation (6 kJ m(-2) d(-1)) had similar light-saturated photosynthetic rates but up to 50% lower stomatal conductance rates, as compared to plants grown without UV-B radiation. Growth responses of Arabidopsis to UV-B radiation included lower leaf area (25%) and biomass (10%) and higher UV-B absorbing compounds (30%) and chlorophyll content (52%). Lower stomatal conductance rates for plants grown with UV-B radiation were, in part, due to lower stomatal density on the adaxial surface. Plants grown with UV-B radiation had more capacity to down regulate photochemical efficiency of photosystem II (PSII) as shown by up to 25% lower phi(PSII) and 30% higher non-photochemical quenching of chlorophyll fluorescence under saturating light. These contributed to a smaller reduction in the maximum photochemical efficiency of PSII (F (v)/F (m)), greater dark-recovery of F (v)/F (m), and higher light-saturated carbon assimilation and stomatal conductance and transpiration rates after a four-hour high light treatment for plants grown with UV-B radiation. Plants grown with UV-B were more tolerant to a 12 day drought treatment than plants grown without UV-B as indicated by two times higher photosynthetic rates and 12% higher relative water content. UV-B-grown plants also had three times higher proline content. Higher tolerance to drought stress for Arabidopsis plants grown under UV-B radiation may be attributed to both increased proline content and decreased stomatal conductance. Growth of Arabidopsis in a UV-B-enhanced light regime increased tolerance to high light exposure and drought stress.

show abstract

Supplementary ultraviolet‐B irradiation reveals differences in stress responses between Arabidopsis thaliana ecotypes*

Abstract: Irradiation of

Cited by 65 publications

References 50 publications

Comparative Cross-Species Alternative Splicing in Plants

Comparative Cross-Species Alternative Splicing in Plants

The E subunit of photosystem I is not essential for linear electron flow and photoautotrophic growth in Arabidopsis thaliana

Response of photosynthesis to high light and drought for Arabidopsis thaliana grown under a UV-B enhanced light regime

Contact Info

Product

Resources

About