Superior visible light antimicrobial performance of facet engineered cobalt doped TiO2 mesocrystals in pathogenic bacterium and fungi

Abstract

Pristine and Co-doped TiO2 mesocrystals have been synthesized via a simple sol–gel method and their antimicrobial activity has been investigated. The antimicrobial performance was evaluated in terms of zone of inhibition, minimum inhibitory concentration (MIC), antibiofilm activity, and effect of UV illumination in liquid media. The Co-doped TiO2 mesocrystals showed very promising MIC of 0.390 μg/mL and 0.781 μg/mL for P. mirabilis and P. mirabilis, respectively. Additionally, the material showed an MIC of 12.5 μg/mL against C. albicans, suggesting its use as antifungal agent. Upon the addition of 10.0 µg/mL of Co-doped TiO2 mesocrystals, the biofilm inhibition% reaches 84.43% for P. aeruginosa, 78.58% for P. mirabilis, and 77.81% for S. typhi, which can be ascribed to the created active oxygen species that decompose the tested microbial cells upon illumination. Thus the fabricated Co-doped TiO2 mesocrystals exhibit sufficient antimicrobial features under visible light, qualifying them for use as antimicrobial agents against pathogenic bacteria and fungi and subsequently inhibit their hazardous effects.

Promising antimicrobial and antibiofilm activities of reduced graphene oxide-metal oxide (RGO-NiO, RGO-AgO, and RGO-ZnO) nanocomposites

“…The sensitive microbial cells (from the antibiolm analysis) were washed with PBS three times and eventually xed with 4.0% glutaraldehyde solution. 48 The preserved microbial cells were regularly cleaned with PBS and repeatedly drained with ethanol of increasing concentrations (30, 50, 70, 90, and 100%) for 15 min each at 28 AE 2 C. 49 Next, the xed samples were xed on an aluminum stub for SEM analysis. The morphological characteristics of the controls (non-treated microbial cells) and the RGO-NiO, RGO-AgO, and RGO-ZnO nanocomposite-treated microbes were observed by SEM examination.…”

“…47 The signicant consequences include the production of holes in the microbial cell wall, which eventually change cell permeability and nally lead to cell death. 48 Reaction mechanism determination by SEM imaging SEM imaging analysis was conducted to explain the potential antimicrobial mechanism against the unicellular fungus C. neoformans, see Fig. 13.…”

Antibacterial and antibiofilm activities of silver-decorated zinc ferrite nanoparticles synthesized by a gamma irradiation-coupled sol–gel method against some pathogenic bacteria from medical operating room surfaces

“…The antimicrobial activity of ZFO NPs and Ag 0.75 @ZFO NPs without and with UV illumination was assessed against the test pathogenic microbe (S. vitulinus) using the optical density method. 47 Bacterial cultures incubated for two hours were adjusted to standard 0. incident on the samples at 37 C. The turbidity of the medium was measured at 600 nm. The inhibition percentage of the tested bacterial pathogens was calculated by eqn (1).…”

Nanobiotic formulations as promising advances for combating MRSA resistance: susceptibilities and post-antibiotic effects of clindamycin, doxycycline, and linezolid

“… 47 The signals resulting from the different points on the sample bombarded by electrons are collected by a detector, amplified, and displayed as an image on a computer monitor. 48 …”