Superficial Zone Extracellular Matrix Extracts Enhance Boundary Lubrication of Self-Assembled Articular Cartilage

Peng, Gordon; McNary, Sean M.; Athanasiou, Kyriacos A.; Reddi, A. Hari

doi:10.1177/1947603515612190

Cited by 7 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HA, glycoproteins (PRG4 or lubricin), and surface active phospholipids (SAPL) working synergistically in forms of lamina splendens are responsible for remarkable boundary lubrication of the cartilage with a reported coefficient of friction of ∼0.005. ,− PRG4 does not adsorb on biomaterials due to the blocking effect of albumin, which is abundantly present in the fluid . This lack of adsorption gives rise to a high coefficient of friction between the tissue and biomaterial, especially during the swing phase of the gait cycle .…”

Section: Resultsmentioning

confidence: 99%

Nanostructured Coating for Biomaterial Lubrication through Biomacromolecular Recruitment

Wan

Zhao

Lin

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Biomaterials employed in the articular joint cavity, such as polycarbonate urethane (PCU) for meniscus replacement, lack of lubrication ability, leading to pain and tissue degradation. We present a nanostructured adhesive coating based on dopaminemodified hyaluronan (HADN) and poly-lysine (PLL), which can reestablish boundary lubrication between the cartilage and biomaterial. Lubrication restoration takes place without the need of exogenous lubricious molecules but through a novel strategy of recruitment of native lubricious molecules present in the surrounding milieu. The biomimetic adhesive coating PLL− HADN (78 nm thickness) shows a high adhesive strength (0.51 MPa) to PCU and a high synovial fluid responsiveness. The quartz crystal microbalance with dissipation monitoring shows the formation of a thick and softer layer when these coatings are brought in contact with the synovial fluid. X-ray photoelectron spectroscopy and ConA-Alexa staining show clear signs of lubricious protein (PRG4) recruitment on the PLL−HADN surface. Effective recruitment of a lubricious protein by PLL−HADN caused it to dissipate only one-third of the frictional energy as compared to bare PCU when rubbed against the cartilage. Histology shows that this reduction makes the PLL−HADN highly chondroprotective, whereas PLL−HA coatings still show signs of cartilage wear. Shear forces in the range of 0.07−0.1 N were able to remove ∼80% of the PRG4 from the PCU−PLL−HA but only 27% from the PCU−PLL−HADN. Thus, in this study, we have shown that surface recruitment and strong adsorption of biomacromolecules from the surrounding milieu is an effective biomaterial lubrication strategy. This opens up new possibilities for lubrication system reconstruction for medical devices.

show abstract

Section: Resultsmentioning

confidence: 99%

Nanostructured Coating for Biomaterial Lubrication through Biomacromolecular Recruitment

Wan

Zhao

Lin

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…To improve retention of SZP in engineered cartilage, native superficial zone cartilage extract, which likely contains binding macromolecules for SZP, was added to the culture media of self-assembled articular cartilage. Groups treated with a low concentration of extract demonstrated greater SZP staining and a boundary mode coefficient of friction of 0.03, which was significantly lower than the coefficient of friction of self-assembled cartilage cultured in the absence of superficial zone cartilage extracts (0.10) (Peng et al, 2016). Combining superficial zone extract with growth factors such as TGF-β1 and BMP-7 could further enhance tribological properties.…”

Section: Bioactive Factorsmentioning

confidence: 87%

“…An increase in SZP secretion does not always cause a decrease in tissue friction coefficients, as SZP must be retained at the cartilage surface to improve boundary lubrication (Peng et al, 2016). To improve retention of SZP in engineered cartilage, native superficial zone cartilage extract, which likely contains binding macromolecules for SZP, was added to the culture media of self-assembled articular cartilage.…”

Section: Bioactive Factorsmentioning

confidence: 99%

The tribology of cartilage: Mechanisms, experimental techniques, and relevance to translational tissue engineering

Link¹,

Salinas²,

et al. 2020

Clinical Biomechanics

Self Cite

View full text Add to dashboard Cite

Diarthrodial joints, found at the ends of long bones, function to dissipate load and allow for effortless articulation. Essential to these functions are cartilages, soft hydrated tissues such as hyaline articular cartilage and the knee meniscus, as well as lubricating synovial fluid. Maintaining adequate lubrication protects cartilages from wear, but a decrease in this function leads to tissue degeneration and pathologies such as osteoarthritis. To study cartilage physiology, articular cartilage researchers have employed tribology, the study of lubrication and wear between two opposing surfaces, to characterize both native and engineered tissues. The biochemical components of synovial fluid allow it to function as an effective lubricant that exhibits shear-thinning behavior. Although tribological properties are recognized to be essential to native tissue function and a critical characteristic for translational tissue engineering, tribology is vastly understudied when compared to other mechanical properties such as compressive moduli. Further, tribometer configurations and testing modalities vary greatly across laboratories. This review aims to define commonly examined tribological characteristics and discuss the structurefunction relationships of biochemical constituents known to contribute to tribological properties in native tissue, address the variations in experimental set-ups by suggesting a move toward standard testing practices, and describe how tissue-engineered cartilages may be augmented to improve their tribological properties.

show abstract

“…Human ECM from allogeneic sources [ 65 ] or cadaveric donors has also shown promise as an engineered material for cartilage regeneration. An acellular three-dimensional interconnected porous scaffold derived from human cartilage ECM improved cell adhesion, proliferation, and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into chondrocyte-like cells over 21 days [ 66 ]. These chondrocyte-like BMSCs were transferred onto another cartilage ECM-derived porous scaffold and implanted subcutaneously into nude mice.…”

Section: Survey Of Engineered Cell–ecm–materials Interactions In Musc...mentioning

confidence: 99%

Engineering Cell–ECM–Material Interactions for Musculoskeletal Regeneration

2023

View full text Add to dashboard Cite

The extracellular microenvironment regulates many of the mechanical and biochemical cues that direct musculoskeletal development and are involved in musculoskeletal disease. The extracellular matrix (ECM) is a main component of this microenvironment. Tissue engineered approaches towards regenerating muscle, cartilage, tendon, and bone target the ECM because it supplies critical signals for regenerating musculoskeletal tissues. Engineered ECM–material scaffolds that mimic key mechanical and biochemical components of the ECM are of particular interest in musculoskeletal tissue engineering. Such materials are biocompatible, can be fabricated to have desirable mechanical and biochemical properties, and can be further chemically or genetically modified to support cell differentiation or halt degenerative disease progression. In this review, we survey how engineered approaches using natural and ECM-derived materials and scaffold systems can harness the unique characteristics of the ECM to support musculoskeletal tissue regeneration, with a focus on skeletal muscle, cartilage, tendon, and bone. We summarize the strengths of current approaches and look towards a future of materials and culture systems with engineered and highly tailored cell–ECM–material interactions to drive musculoskeletal tissue restoration. The works highlighted in this review strongly support the continued exploration of ECM and other engineered materials as tools to control cell fate and make large-scale musculoskeletal regeneration a reality.

show abstract

Superficial Zone Extracellular Matrix Extracts Enhance Boundary Lubrication of Self-Assembled Articular Cartilage

Abstract: Friction coefficients of self-assembled articular cartilage constructs were reduced by adding extracted superficial zone ECM into the culture medium of self-assembled articular cartilage constructs.

Cited by 7 publications

References 31 publications

Nanostructured Coating for Biomaterial Lubrication through Biomacromolecular Recruitment

Nanostructured Coating for Biomaterial Lubrication through Biomacromolecular Recruitment

The tribology of cartilage: Mechanisms, experimental techniques, and relevance to translational tissue engineering

Engineering Cell–ECM–Material Interactions for Musculoskeletal Regeneration

Contact Info

Product

Resources

About