Supercritical fluid extraction and chromatography for fat-soluble vitamin analysis

Turner, Charlotta; King, Jerry W.; Mathiasson, Lennart

doi:10.1016/s0021-9673(01)01082-2

Cited by 163 publications

(82 citation statements)

References 92 publications

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“…SFE has also been described as a desirable alternative to solvent extraction of carotenoids as it enhances the speed and efficiency of extraction and eliminates concentration steps (Turner et al 2001). Supercritical carbon dioxide (SC-CO 2 ) is the most commonly used extraction agent because of its non-toxicity, chemical inertness, and low cost and easy availability.…”

Section: Extraction and Pretreatment Of Samplesmentioning

confidence: 99%

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

et al. 2014

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Carotenoids are increasingly drawing the attention of researchers as a major natural food color due to their inherent nutritional characteristics and the implicated possible role in prevention and protection against degenerative diseases. In this report, we review the role of red pepper as a source for natural carotenoids. The composition of the carotenoids in red pepper and the application of different methodologies for their analysis were discussed in this report. The stability of red pepper carotenoids during post-harvest processing and storage is also reviewed. This review highlights the potential of red pepper carotenoids as a source of natural food colors and also discusses the need for a standardized approach for the analysis and reporting of composition of carotenoids in plant products and designing model systems for stability studies.

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Section: Extraction and Pretreatment Of Samplesmentioning

confidence: 99%

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

et al. 2014

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“…Tab. 3. The presence of the ethanol entrainer significantly improves the solubility [7,18], however, a very high amount of entrainer might limit mass transfer diffusion. In some cases, it creates a two-phase system (liquid and supercritical fluid) resulting in a lower amount of lutein in the extract [7,19].…”

Section: Effect Of Co 2 Flow Ratementioning

confidence: 99%

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂

et al. 2012

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A pretreatment process using alcohol for the removal of chlorophyll a, b and b-carotene from Chlorella vulgaris was developed to improve the yield and selectivity of lutein in the extract obtained by supercritical carbon dioxide extraction. Supercritical carbon dioxide extraction was carried out after pretreatment in the pressure range of 20 to 40 MPa and the temperature range of 40 to 80°C. Ethanol and methanol were selected as elution solvents, of which ethanol was found most suitable for the elution, or pretreatment, process. The amounts of lutein and other compounds were analyzed by HPLC with the mixture of methanol and THF as the mobile phase. The amount of lutein in the extract increased with pressure, but decreased with extraction temperature. The highest recovery percentage and the selectivity of lutein were around 52.9 ± 0.02 % and 43.1 ± 0.02 %, respectively, obtained from supercritical carbon dioxide extraction with pretreatment and ethanol entrainer at 40 MPa and 40°C.

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“…By using supercritical fluids with low viscosity and high diffusivity, such as supercritical carbon dioxide (scCO 2 ), as mobile phase, SFC exhibits some interesting features [12], such as high separation efficiency, high flow-rates and thus reduced analysis times. Beside the successful achievements in chiral separations, SFC has also showed great potential in the separation and isolation of active components in TCMs and natural products, such as lipids [13], vitamins [14], ginkgolides [15], triterpenoids [16], regioisomeric spirostanol saponin diastereomers [17]. However, very few applications have been focused on the separation of TSs using SFC [18,19].…”

Section: Introductionmentioning

confidence: 99%

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Huang

Zhang

Zhou

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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a b s t r a c tTriterpenoid saponins (TSs) are the most important components of some traditional Chinese medicines (TCMs) and have exhibited valuable pharmacological properties. In this study, a rapid and efficient method was developed for the separation of kudinosides, stauntosides and ginsenosides using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The separation conditions for the selected TSs were carefully optimized after the initial screening of eight stationary phases. The best compromise for all compounds in terms of chromatographic performance and MS sensitivity was obtained when water (5-10%) and formic acid (0.05%) were added to the supercritical carbon dioxide/MeOH mobile phase. Beside the composition of the mobile phase, the nature of the make-up solvent for interfacing SFC with MS was also evaluated. Compared to reversed phase liquid chromatography, the SFC approach showed higher resolution and shorter running time. The developed SFC-MS methods were successfully applied to the separation and identification of TSs present in Ilex latifolia Thunb., Panax quinquefolius L. and Panax ginseng C.A. Meyer. These results suggest that this SFC-MS approach could be employed as a useful tool for the quality assessment of natural products containing TSs as active components.

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Supercritical fluid extraction and chromatography for fat-soluble vitamin analysis

Cited by 163 publications

References 92 publications

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

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Supercritical fluid extraction and chromatography for fat-soluble vitamin analysis

Cited by 163 publications

References 92 publications

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO2

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

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Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂