Supercritical fluid chromatography for the enantioseparation of pharmaceuticals

Impact of injection solvents on supercritical fluid chromatography

“…The low viscosity also enables the use of several coupled columns as well as high flow rates leading to short analysis times [1].…”

Evaluation and comparison of three different separation techniques for analysis of retroamide enantiomers and their biological evaluation against h-P2X7 receptor

“…Its main advantages (e.g. the low viscosity and high diffusivity of carbon dioxide (CO 2 ) based mobile phase offering higher efficiencies, shorter run time and faster column equilibration) permit the use of higher flow rates and permit an important decrease of the cycle time [13]. The combination of these effects results in an important increase of the production rates of this purification process.…”

Effects of supercritical fluid chromatography conditions on enantioselectivity and performance of polyproline-derived chiral stationary phases

“…SFC conditions, using CO 2 as a main component of the mobile phase, parallels normal phase HPLC [17]. The low viscosity and higher diffusivity of the SFC mobile phase shifts the optimum linear velocity to higher values compared to liquids [18]. Thus, higher flow rates can be used, which reduce analysis and equilibration times, and increases high-throughput capacity [19].…”

“…Among them, the separated enantiomers can be easily recovered after separation by evaporation of the low amount of co-solvent used. This involves a significant reduction in waste generation and disposal [18].…”