Supercritical CO2 extraction of hemp (Cannabis sativa L.) seed oil

Aladić, Krunoslav; Jarni, Kristjan; Barbir, Tina; Vidović, Senka; Vladić, Jelena; Bilić, Mate; Jokić, Stela

doi:10.1016/j.indcrop.2015.07.016

Cited by 118 publications

(92 citation statements)

References 19 publications

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“…It has 2000 t/ year processing ability and works in a pressure range 20-53 MPa Rój et al 2013). SFE is solvent and waste-free, is faster than conventional liquid-liquid methods, and with easy parameter control, provides a certain selectivity (Meyer et al 2012;Aladić et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In a significant number of publications about scCO 2 extraction of raw plants or their parts, we can find that several authors described matrix effects (Aladić et al 2015;Araus et al 2009;Azmir et al 2013), and others discuss optimized reaction parameters in the production of plant oils using, for example, response surface methodology (Rój and Skowroński 2006a, b;Azmir et al 2013;Stamenic et al 2010;Watros et al 2013;Da Porto et al 2012a, b;Ara et al 2015). Most of them used scCO 2 laboratory-scale extraction installation (up to 2 L extraction vessel) to obtain oils from the most popular fruits, vegetables, or herbs, e.g., grapes, olives, and sunflowers (Yang et al 2011;Rai et al 2016;Aladić et al 2015;Milić et al 2015;Duba and Fiori 2015;Del Valle 2015;Da Porto et al 2012a, b), but only a few of them used supercritical CO 2 industry-scale extraction plant to obtain oils, especially from berry seeds .…”

Section: Introductionmentioning

confidence: 99%

“…Most of them used scCO 2 laboratory-scale extraction installation (up to 2 L extraction vessel) to obtain oils from the most popular fruits, vegetables, or herbs, e.g., grapes, olives, and sunflowers (Yang et al 2011;Rai et al 2016;Aladić et al 2015;Milić et al 2015;Duba and Fiori 2015;Del Valle 2015;Da Porto et al 2012a, b), but only a few of them used supercritical CO 2 industry-scale extraction plant to obtain oils, especially from berry seeds .…”

Section: Introductionmentioning

confidence: 99%

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Fatty Acids Analysis Using Gas Chromatography-Mass Spectrometer Detector (GC/MSD) - Method Validation Based on Berry Seed Extract Samples

2017

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Oily seed extracts were used as research samples in Analytical Laboratory of Raw Materials and Plant Products. Supercritical carbon dioxide extraction on an industrial scale was used to prepare extracts from raw plant materials, usually berry seeds: blackcurrant, raspberry, strawberry, and chokeberry. Gas chromatography analysis was performed on Agilent equipment with single quadrupole mass spectrometer detector and split/splitless injector. Fatty acids (FAs) were determined using indirect method, where they were converted to c o r r e s p o n d i n g m e t h y l e s t e r s ( FA M E s ) d u r i n g trimethylsulfonium hydroxide solution reaction. Comparing the mass spectrum and retention time peak, more than 30 compounds in different oily extracts were qualitatively identified. Quantification of individual fatty acids was based on two different methods. Firstly, it was based on the received peak area, and the results were normalized without correction factor. Secondly, the quantification was based on external calibration curve, for 34 identified fatty acids. The method was validated, and the results, e.g., linearity, precision, limit of detection (LOD), and limit of quantification (LOQ), were presented.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fatty Acids Analysis Using Gas Chromatography-Mass Spectrometer Detector (GC/MSD) - Method Validation Based on Berry Seed Extract Samples

2017

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“…High seed oil content, fast plant growth and low agricultural inputs make hemp a cost-effective crop for biodiesel production. Cold pressing [24][25][26], supercritical carbon dioxide extraction [26][27][28][29] and solvent extraction [3] have been applied so far for extracting the oil from hemp seeds. Kostić et al [3] have studied the impact of the process factors on the hempseed oil (HSO) yield reached by solvent extraction using the RSM coupled with a FFD with replication.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Box-Behnken, Face Central Composite and Full Factorial Designs in Optimization of Hempseed Oil Extraction by n-Hexane: a Case Study

Stamenković¹,

Kostić²,

Radosavljević³

et al. 2018

Period. Polytech. Chem. Eng.

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“…Essas substâncias fazem parte de um grupo chamado canabinóides, que são terpenos fenólicos [3] produzidos pela espécie [4] , sendo mais de 66 já identificados [5] . Atualmente as indústrias vêm utilizando o dióxido de carbono em condições supercríticas (CO2-SC), como solvente, para obter extratos medicinais [6,7,8,9] porém, apesar da eficiência [10,11] , ainda vêm sendo utilizando solventes orgânicos [12,13] nesta aplicação.Em materiais vegetais brutos a maior parte destes canabinóides então na forma de ácido canabidiol (CBDA) e ácido  9 -tetrahidrocanabinol ( 9 -THCA) [14] . Um estudo demonstrou uma porcentagem de  9 -THCA aproximadamente 5,5 vezes maior que de  9 -THC [15] .…”

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Análise de canabinóides neutros em derivados de Cannabis via GC/MS

Grijó¹,

Cardozo²

2017

Blucher Chemical Engineering Proceedings

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ResumoAtualmente a medicina tem grande interesse na espécie Cannabis sativa L., especialmente em dois de seus canabinóides neutros (CN): canabidiol (CBD) e  9 -tetrahidrocanabinol ( 9 -THC). Em parceria com a Polícia Científica de Curitiba -Brasil, foram feitas análises, via cromatografia gasosa e espectrometria de massas (GC/MS), da composição e o comportamento térmico de CN em derivados dessa espécie, com solvente polar e apolar. Testou-se também, em sementes, uma metodologia industrial de limpeza para remover resíduos de CN aderidos por contato com outras partes da planta. A partir de curvas de calibração, utilizando padrões de CBD,  9 -THC e canabinol (CBN), foi possível fazer análises quantitativas da influência térmica e da metodologia de lavagem. Esses e outros canabinóides foram identificados pela razão massa carga (m/z). Foi comprovado que o aquecimento de amostras aumenta o potencial medicinal, mas também transforma o  9 -THC em CBN e/ou  8 -tetraidrocanabinol ( 8 -THC), e o CBD em canabielsoin (CBE) e/ou  9 -THC. O extrato apolar apresentou maior concentração de CN. Demonstrou-se também a diminuição de CN nas sementes pelo método de lavagem industrial, bem como o rendimento em óleo esperado em referências. Com esse trabalho foi possível demonstrar a importância de conhecimento do comportamento dos CN em derivados de Cannabis. AbstractCurrently the medicine has great interest in the species Cannabis sativa L., especially in two of its neutral cannabinoids (CN): cannabidiol (CBD) and  9 -tetrahydrocanabinol ( 9 -THC). In partnership with the Scientific Policy of Curitiba -Brazil, analyses had been made, with gas chromatography and mass spectrometry (GC/MS), of the composition and the thermal behavior of CN in derivatives of this species, with polar and nonpolar solvent. It was also tested in seeds an industrial cleaning method to remove CN residues adhered by contact with other parts of the plant. From the calibration curves using the standards CBD,  9 -THC and canabinol (CBN), it was possible to make quantitative analysis of the thermal influence and the cleaning method. Those and others canabinnoids were identified by mass/charge ratio (m/z). It was proven that the heating samples increases the medicinal potential, but also transforms  9 -THC in CBN and/or  8 -tetraidrocanabinol ( 8 -THC), and CBD in canabielsoin (CBE) and/or  9 -THC. The nonpolar extract has a higher concentration of CN. Cleaning method reduce CN in seeds, as well oil yield expected results. With this work it was possible to demonstrate the importance of knowledge of the CN behavior in Cannabis derivatives.Keywords (Palavras chaves): cannabinoids, gas chromatography, mass spectrometry. IntroduçãoAtualmente no Brasil se tem grande interesse em duas substâncias derivadas da espécie Cannabis sativa L., o canabidiol (CBD) e o  9 -tetrahidrocanabinol ( 9 -THC) [1] , ou seja, (m/z)CBD = (231; 174; 246; 121; 193) e (m/z) 314; 231; 271; 243; 258) [2] . Essas substâncias fazem parte de um grupo chamado canabinóides, que s...

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Supercritical CO2 extraction of hemp (Cannabis sativa L.) seed oil

Cited by 118 publications

References 19 publications

Fatty Acids Analysis Using Gas Chromatography-Mass Spectrometer Detector (GC/MSD) - Method Validation Based on Berry Seed Extract Samples

Fatty Acids Analysis Using Gas Chromatography-Mass Spectrometer Detector (GC/MSD) - Method Validation Based on Berry Seed Extract Samples

Comparison of Box-Behnken, Face Central Composite and Full Factorial Designs in Optimization of Hempseed Oil Extraction by n-Hexane: a Case Study

Análise de canabinóides neutros em derivados de Cannabis via GC/MS

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