Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy

Abstract

Purpose To deliver insoluble natural compounds into colon cancer cells in a controlled fashion. Materials and methods Curcumin (CM)–silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO 2 (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed. Results CM-SF NPs (<100 nm) with controllable particle size were prepared by SEDS. CM-SF NPs had a time-dependent intracellular uptake ability, which led to an improved inhibition effect on colon cancer cells. Interestingly, the anticancer effect of CM-SF NPs was improved, while the side effect on normal human colon mucosal epithelial cells was reduced by a concentration of ~10 μg/mL. The anticancer mechanism involves cell-cycle arrest in the G 0 /G 1 and G 2 /M phases in association with inducing apoptotic cells. Conclusion The natural compound-loaded SF nanoplatform prepared by SEDS indicates promising colon cancer-therapy potential.

Colorectal cancer combination therapy using drug and gene co-delivered, targeted poly(ethylene glycol)-&epsilon;-poly(caprolactone) nanocarriers

“… 40 In vivo pharmacokinetic study showed the biphasic elimination behaviors of the 5-FU-loaded NCs. 41 , 42 The fact that the drug-loaded NCs follow a biphasic elimination pattern is consistent with data suggesting that a large proportion of the encapsulated 5-FU is lost from the NCs following administration. The second phase of elimination may be due to a fraction of the 5-FU that remains more tightly associated with the inner core of the NCs.…”

Nanocurcumin: A Promising Candidate for Therapeutic Applications

“…Also, curcumin silk fibroin (CUR-SF) nanoparticles provided a more stable delivery to colon cancer cells and produced a strong anticancer effect than it's freeform in HCT116 cells. This study concludes controlled release of CUR-SF can able to improve a curcumin cellular uptake into cancer cells and reduce the cytotoxicity to normal cells (Xie et al, 2017).…”

“…Exhibited strong wound healing and long blood circulation, suppression of tumor growth, higher growth inhibition in cancer cells than free curcumin, and increased the cellular uptake and better anticancer activity Li et al, 2012;Anitha et al, 2014;Chaurasia et al, 2016;Xie et al, 2017;Moballegh Nasery et al, 2020…”

<p>Improving Solubility and Bioavailability of Breviscapine with Mesoporous Silica Nanoparticles Prepared Using Ultrasound-Assisted Solution-Enhanced Dispersion by Supercritical Fluids Method</p>

“…However, unlike other main SCF techniques, the nozzle of SEDS presents a coaxial design with a mixing length, in order to produce super-saturated solutions, facilitate precipitation of the solid in the form of small particles with high dispersion. 32 In addition, we added an ultrasonic system to enhance the effect of SEDS technology which can obtain further smaller particles and make the drug more evenly loaded into the carriers.…”